Relationships among the founding ancestral genomes involved in the origin of sugarcane (Saccharum spp.) based on FISH and GISH comparative analysis

Soares, Nina Reis; Oliveira, Gleicy K.; Xavier, Mauro Alexandre; Creste, Silvana; Vieira, Maria Lúcia Carneiro

doi:10.1007/s40415-023-00914-8

Cited by 1 publication

(1 citation statement)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Saccharum complex consists of two wild species, S. spontaneum (2n = 8x = 40-128, x = 8, 9 or 10) and S. robustum (2n = 60, x = 10, 80-200 chromosomes), and four cultivated species, S. officinarum (2n = 8x = 80, x = 10), S. sinense, S. barberi, and S. edule, all polyploids (Cursi et al, 2022;D'Hont et al, 1998). S. robustum is the most likely precursor of sugarcane and anthropic selection of plants for sweetness and low fiber content produced the first S. officinarum clones (Pompidor et al, 2021;Soares et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

First investigation into the genetic control of meiosis in sugarcane

Reis Soares,

Costa,

Marques

et al. 2024

The Plant Journal

Self Cite

View full text Add to dashboard Cite

SUMMARYThe sugarcane (Saccharum spp.) genome is one of the most complex of all. Modern varieties are highly polyploid and aneuploid as a result of hybridization between Saccharum officinarum and S. spontaneum. Little research has been done on meiotic control in polyploid species, with the exception of the wheat Ph1 locus harboring the ZIP4 gene (TaZIP4‐B2) which promotes pairing between homologous chromosomes while suppressing crossover between homeologs. In sugarcane, despite its interspecific origin, bivalent association is favored, and multivalents, if any, are resolved at the end of prophase I. Thus, our aim herein was to investigate the purported genetic control of meiosis in the parental species and in sugarcane itself. We investigated the ZIP4 gene and immunolocalized meiotic proteins, namely synaptonemal complex proteins Zyp1 and Asy1. The sugarcane ZIP4 gene is located on chromosome 2 and expressed more abundantly in flowers, a similar profile to that found for TaZIP4‐B2. ZIP4 expression is higher in S. spontaneum a neoautopolyploid, with lower expression in S. officinarum, a stable octoploid species. The sugarcane Zip4 protein contains a TPR domain, essential for scaffolding. Its 3D structure was also predicted, and it was found to be very similar to that of TaZIP4‐B2, reflecting their functional relatedness. Immunolocalization of the Asy1 and Zyp1 proteins revealed that S. officinarum completes synapsis. However, in S. spontaneum and SP80‐3280 (a modern variety), no nuclei with complete synapsis were observed. Importantly, our results have implications for sugarcane cytogenetics, genetic mapping, and genomics.

show abstract