Relationships between endogenous hormonal content and direct somatic embryogenesis in Prunus persica L. Batsch cotyledons

Pérez‐Jiménez, Margarita; Cantero-Navarro, Elena; Acosta, Manuel; Cos-Terrer, José

doi:10.1007/s10725-013-9822-7

Cited by 15 publications

(12 citation statements)

References 30 publications

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“…Salicylic acid, a phenolic derivative, is considered to be a potent plant hormone because of its diverse regulatory roles in plants. It has direct involvement in plant growth, thermogenesis, flower induction and uptake of ions (Pérez-Jiménez et al ., 2013 ). Enhancement of the levels of chlorophyll and carotenoid pigments and photosynthetic rate, as well as modifying the activity of some of the important enzymes, are other roles assigned to SA, all of which enhance plant growth and yield (Hayat et al ., 2007 ).…”

Section: Discussionmentioning

confidence: 99%

Growth‐promoting Sphingomonas paucimobilis ZJSH1 associated with Dendrobium officinale through phytohormone production and nitrogen fixation

Yang

Zhang

Cao

et al. 2014

Microbial Biotechnology

109

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Growth-promoting Sphingomonas paucimobilis ZJSH1, associated with Dendrobium officinale, a traditional Chinese medicinal plant, was characterized. At 90 days post-inoculation, strain ZJSH1 significantly promoted the growth of D. officinale seedlings, with increases of stems by 8.6% and fresh weight by 7.5%. Interestingly, the polysaccharide content extracted from the inoculated seedlings was 0.6% higher than that of the control. Similar growth promotion was observed with the transplants inoculated with strain ZJSH1. The mechanism of growth promotion was attributed to a combination of phytohormones and nitrogen fixation. Strain ZJSH1 was found using the Kjeldahl method to have a nitrogen fixation activity of 1.15 mg l−1, which was confirmed by sequencing of the nifH gene. Using high-performance liquid chromatography-mass spectrometry, strain ZJSH1 was found to produce various phytohormones, including salicylic acid (SA), indole-3-acetic acid (IAA), Zeatin and abscisic acid (ABA). The growth curve showed that strain ZJSH1 grew well in the seedlings, especially in the roots. Accordingly, much higher contents of SA, ABA, IAA and c-ZR were detected in the inoculated seedlings, which may play roles as both phytohormones and ‘Systemic Acquired Resistance’ drivers. Nitrogen fixation and secretion of plant growth regulators (SA, IAA, Zeatin and ABA) endow S. paucimobilis ZJSH1 with growth-promoting properties, which provides a potential for application in the commercial growth of D. officinale.

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Section: Discussionmentioning

confidence: 99%

Growth‐promoting Sphingomonas paucimobilis ZJSH1 associated with Dendrobium officinale through phytohormone production and nitrogen fixation

Yang

Zhang

Cao

et al. 2014

Microbial Biotechnology

109

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“…In turn, Jiménez et al ( 2005 ) suggested that ABA is not directly involved in the very initial stages of the embryogenic development of the carrot callus tissue, since the endogenous level of the hormone did not change in response to the presence/absence of 2,4-D. Thi and Pleschka ( 2005 ) reported a positive relationship between the endogenous contents of ABA in petioles and somatic embryogenesis of some Daucus species. In turn, no significant differences in the ABA content were observed between the embryogenic and non-embryogenic peach cotyledons used as initial explants (Pérez-Jiménez et al 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Profiles of endogenous ABA, bioactive GAs, IAA and their metabolites in Medicago truncatula Gaertn. non-embryogenic and embryogenic tissues during induction phase in relation to somatic embryo formation

Kępczyńska

Orłowska

2021

Planta

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Main conclusion During the 3-week-long induction phase, when M. truncatula cells leaf explants from non-embryogenic genotype (M9) and embryogenic variant (M9-10a) were forming the callus, biosynthesis and degradation of ABA, Gas and IAA proceeded at different levels. Induction of embryo formation is related to a lower ABA content, compared to the content of IAA and that of total bioactive GAs. Abstract Endogenous phytohormones are involved in the regulation of zygotic embryogenesis, but their role, especially of ABA, a plant growth inhibitor, in inducing somatic embryogenesis (SE) in angiosperms is still incompletely known. To arrive a better understanding of the ABA role in the process, we analyzed simultaneously and in detail changes in the contents of both ABA and five bioactive GAs (GA4, GA7, GA1, GA3, GA6) and IAA in M. truncatula non-embryogenic M9 (NE) and embryogenic M9-10a (E) genotypes. The initial leaf explants of both genotypes, and particularly NE, contained many times more ABA compared to the total bioactive GAs or IAA. In tissues during the entire 21-day induction all the hormones mentioned and their metabolites or conjugates were present; however, their contents were found to differ between the lines tested. The ABA level in primary explants of NE genotype was more than two times higher than that in E genotype. An even larger difference in the ABA content was found on the last day (day 21) of the induction phase (IP); the ABA content in E callus was over six times lower than in NE callus. In contrast, the IAA and GAs contents in primary explants of both genotypes in relation to ABA were low, but the contents of IAA and GAs exceeded that of ABA in the M9-10a tissues on the last day of IP. It is shown for the first time that endogenous ABA together with endogenous bioactive GAs and IAA is involved in acquisition of embryogenic competence in Medicago truncatula leaf somatic cells. These findings have a strong functional implication as they allow to improve the SE induction protocol.

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“…The results showed that auxin response factor ( ARF ) [11,13,14], leafy cotyledon ( LEC ) [10,15], Wuschel ( WUS ) [16,17,18], somatic embryogenesis receptor kinase ( SERK ) [8], shoot meristemless ( STM ), and baby boom ( BBM ) [10,19,20] were involved in SE. In addition, the salicylic acid (SA) and jasmonic acid (JA) signaling pathways were also predicted to regulate SE [12,21,22,23,24,25,26,27].…”

Section: Introductionmentioning

confidence: 99%

Metabolome and Transcriptome Association Analysis Reveals Dynamic Regulation of Purine Metabolism and Flavonoid Synthesis in Transdifferentiation during Somatic Embryogenesis in Cotton

Guo

Zhang

et al. 2019

IJMS

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Plant regeneration via somatic embryogenesis (SE) is a key step during genetic engineering. In the current study, integrated widely targeted metabolomics and RNA sequencing were performed to investigate the dynamic metabolic and transcriptional profiling of cotton SE. Our data revealed that a total of 581 metabolites were present in nonembryogenic staged calli (NEC), primary embryogenic calli (PEC), and initiation staged globular embryos (GE). Of the differentially accumulated metabolites (DAMs), nucleotides, and lipids were specifically accumulated during embryogenic differentiation, whereas flavones and hydroxycinnamoyl derivatives were accumulated during somatic embryo development. Additionally, metabolites related to purine metabolism were significantly enriched in PEC vs. NEC, whereas in GE vs. PEC, DAMs were remarkably associated with flavonoid biosynthesis. An association analysis of the metabolome and transcriptome data indicated that purine metabolism and flavonoid biosynthesis were co-mapped based on the Kyoto encyclopedia of genes and genomes (KEGG) database. Moreover, purine metabolism-related genes associated with signal recognition, transcription, stress, and lipid binding were significantly upregulated. Moreover, several classic somatic embryogenesis (SE) genes were highly correlated with their corresponding metabolites that were involved in purine metabolism and flavonoid biosynthesis. The current study identified a series of potential metabolites and corresponding genes responsible for SE transdifferentiation, which provides a valuable foundation for a deeper understanding of the regulatory mechanisms underlying cell totipotency at the molecular and biochemical levels.

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Relationships between endogenous hormonal content and direct somatic embryogenesis in Prunus persica L. Batsch cotyledons

Cited by 15 publications

References 30 publications

Growth‐promoting Sphingomonas paucimobilis ZJSH1 associated with Dendrobium officinale through phytohormone production and nitrogen fixation

Growth‐promoting Sphingomonas paucimobilis ZJSH1 associated with Dendrobium officinale through phytohormone production and nitrogen fixation

Profiles of endogenous ABA, bioactive GAs, IAA and their metabolites in Medicago truncatula Gaertn. non-embryogenic and embryogenic tissues during induction phase in relation to somatic embryo formation

Metabolome and Transcriptome Association Analysis Reveals Dynamic Regulation of Purine Metabolism and Flavonoid Synthesis in Transdifferentiation during Somatic Embryogenesis in Cotton

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