Relationships between Hha1 Calpastatin Gene Polymorphism, Growth Performance, and Meat Characteristics of Awassi Sheep

Jawasreh, Khaleel; Al-Amareen, Ahmad H.; Aad, Pauline Y.

doi:10.3390/ani9090667

Cited by 16 publications

(21 citation statements)

References 16 publications

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“…In this study, two CAST genotypes were detected with frequencies of 0.65 and 0.35 for MN and NN genotype, respectively. These results are in agreement with previously reported data in different breeds of sheep [11]. In this study, lambs with heterozygous CAST gene were found to have significantly higher percentages of neutrophils and lower percentages of lymphocytes.…”

Section: Discussionsupporting

confidence: 94%

“…Whether this difference has a significant effect on disease resistance in MN lambs requires further investigation. Previous research in Awassi sheep has reported superior performance of MN CAST genotype [11].…”

Section: Discussionmentioning

confidence: 99%

“…In this study, MN CAST lambs were found to have significantly higher levels of T3 than that in NN CAST lambs. Degradation of protein as a result of high levels of T3 may explain the major effect of CAST gene on meat tenderness claimed by many authors [11].…”

Section: Discussionmentioning

confidence: 99%

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Effects of calpastatin gene polymorphism on hematology and selected serum biochemical parameters in Awassi lambs

Jawasreh

Ismail

2019

J Adv Vet Anim Res

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Objective: This study was conducted to investigate the effect of MspI polymorphism in the calpastatin (CAST) gene on hematology and selected serum biochemical parameters in Awassi lambs. Materials and Methods: CAST genotypes of 31 Awassi lambs were determined using polymerase chain reaction—restricted fragment length polymorphism method. Hematology, serum biochemical analyses, serum levels of triiodothyronine, thyroxine, and cortisol were determined using routine laboratory procedures. Results: Two CAST genotypes were detected with frequencies of 0.65 and 0.35 for MN (three major bands of 622, 336, and 268 bp) and NN (two major bands of 336 and 268 bp), respectively. Allele frequencies were 0.49 and 0.51 for M and N alleles, respectively. Animals with MN MspI CAST genotype had significantly ( p < 0.05) higher neutrophil percentage and neutrophil to lymphocyte ratio but, significantly ( p < 0.05) lower lymphocyte percentage and neutrophil to lymphocyte ratio than NN MspI CAST genotype. Serum T3 and cortisol concentrations were significantly ( p < 0.05) higher in MN MspI CAST genotype than the NN MspI CAST genotype. Conclusion: Results of this study indicate that CAST gene heterozygous individuals are healthier than homozygous individual, which may explain the superiority of the CAST gene heterozygous animals in growth performance.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

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Effects of calpastatin gene polymorphism on hematology and selected serum biochemical parameters in Awassi lambs

Jawasreh

Ismail

2019

J Adv Vet Anim Res

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“…Awassi (AW) is a multipurpose fat-tailed sheep breed, well adapted to harsh environmental conditions, and the predominant sheep breed in Jordan, Lebanon, and the Middle East. The AW sheep breed suffers from inferior carcass merit and poor leg muscling [1]. With consumers requiring meat with more lean and less fat without compromising juiciness and flavor [2], heavier carcasses could be advantageous for producers by providing higher profit from meat sales and for consumers by supplying more mature meat with better quality [3].…”

Section: Introductionmentioning

confidence: 99%

Growth performance and meat characteristics of the first filial Awassi Rambouillet callipyge ram lambs

2019

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Aim: This study was designed to introduce the callipyge (CLPG) and 50% of Rambouillet sheep genes to improve meat quality and quantity of Awassi (AW) sheep. Materials and Methods: The CLPG mutation was introduced into the AW sheep through frozen semen of homozygous Rambouillet rams for the CLPG mutation. Four ram lambs from the first-generation Rambouillet callipyge Awassi (F1-RCA) and five from pure AW were recruited for a fattening trial conducted in individual pens using standard ration, following which ram lambs were slaughtered for carcass and meat evaluation. Results: Final body weight, dry matter intake, average daily gain, and feed conversion ratio were significantly higher in F1-RCA than AW. Hot and cold carcass weights and the other carcass cuts' weights, except for the fat tail, were heavier in F1-RCA than AW. There was no difference in dressing percentage between the two genetic groups (p>0.05). All non-carcass components' weights, except spleen, kidney, and testis, were higher in F1-RCA. Total lean, total bone, and intermuscular fat weight were greater in F1-RCA, but bone-to-lean ratio was lower in F1-RCA when compared with AW (p<0.01). No differences (p>0.05) were observed in all meat quality parameters for muscle longissimus with the exception of pH, redness color, and tenderness that were lower (p<0.05) in F1-RCA than AW. F1-RCA lambs had larger longissimus muscle area (30.9 vs. 16.9 cm2) and less leg fat depth (11.1 vs. 17.4 mm). Conclusion: The implications of this research show the potential of 50% of Rambouillet genes and the CLPG mutation to improve growth and meat characteristics in AW-Rambouillet crosses and can be used further to develop a meat-type AW with improved productivity and muscle mass.

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“…The allelic frequencies of alleles M and N were 0.81 and 0.19, Determination of the Polymorphism of the Booroola Fecundity B, Callipyge and Calpastatin Genes in Assaf Sheep Breed... respectively (Table 4). These results show that the desired MN genotype which is associated with satisfactory weight gain in lambs and meat tenderness after slaughter Palmer et al, 1998) (Jawasreh et al, 2017).…”

Section: Resultsmentioning

confidence: 65%

Determination of the Polymorphism of the Booroola Fecundity B, Callipyge and Calpastatin Genes in Assaf Sheep Breed by PCR-RFLP Method

Darissa

Irekat

2020

ASD

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Background: The development of local sheep breeds that have high fecundity levels, high meat content and high growth rates would help satisfy the Palestinian demand of meat and lower its price. It has been reported that mutant alleles of the genes Booroola fecundity (FecB), Callipyge (CLPG) and calpastatin (CAST) are associated with high litter size, larger muscular rumps and higher tenderness of meat after sheep slaughter, respectively. PCR-RFLPs methods have been established to facilitate the survey of sheep breeds for such mutants.Methods: In this study, 117 blood samples of the most common sheep breed in Palestine, Assaf, were collected from Bethlehem and Jenin districts. Genomic DNA was extracted and purified from the blood samples using the salting out method. The genotypic ratios and allelic frequencies of the FecB, CLPG and CAST genes were determined by PCR-RFLP method. Result: The results show that the mutant allele B of the FecB gene has a very low frequency among the screened sheep with 0.01 in Bethlehem and 0.07 in Jenin. Also, 94.4% of the tested sheep have the wildtype genotype AA for the CLPG gene with a frequency of 0.95 for allel A. Moreover, the desired heterzygous genotype MN for the CAST gene is 38.4% with an allelic frequency of 0.81 for the mutant allel M. These results would assist establishing sheep breeding programs with the appropriate parental genotypes to improve the livestock sector in the country.

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Relationships between Hha1 Calpastatin Gene Polymorphism, Growth Performance, and Meat Characteristics of Awassi Sheep

Cited by 16 publications

References 16 publications

Effects of calpastatin gene polymorphism on hematology and selected serum biochemical parameters in Awassi lambs

Effects of calpastatin gene polymorphism on hematology and selected serum biochemical parameters in Awassi lambs

Growth performance and meat characteristics of the first filial Awassi Rambouillet callipyge ram lambs

Determination of the Polymorphism of the Booroola Fecundity B, Callipyge and Calpastatin Genes in Assaf Sheep Breed by PCR-RFLP Method

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