Relationships between intracisternal type A and extracellular oncornavirus-like particles produced in murine MOPC-460 myeloma cells

Robertson, Donald; Yau, Peter; Dobbertin, D C; Sweeney, Tina; Thach, Sigrid S.; Brendler, Therese; Thach, Robert E.

doi:10.1128/jvi.18.1.344-355.1976

Cited by 25 publications

(24 citation statements)

References 38 publications

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“…Initial characterizations of the MAV and solid tumor A-type particles produced by MOPC-460 plasmacytomas involved analyses of the reverse transcriptase, RNA, and proteins associated with them (32,33). Although the proteins bore certain similarities to those of standard MuLV (compare panels A, B, and F of Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Gradients were fractionated by puncturing the tubes, and samples were frozen. Intracisternal A particles were prepared from solid tumors as described previously (33).…”

Section: Materials and Metmodsmentioning

confidence: 99%

“…It was previously reported that intracisternal A-type particles isolated from murine MOPC-460 solid tumors contain an RNA-dependent DNA polymerase (reverse transcriptase), as well as RNA which is genetically related to the RNAs of murine leukemia viruses (MuLV) (32,33).…”

mentioning

confidence: 99%

“…The MAV particles contain 35S and 70S RNA, in addition to larger amounts of degraded RNA, which is indistinguishable from the RNA of intracisternal A particles. Moreover, the 76,000dalton proteins contained in both MAV and solid tumor A-type particles have identical cyanogen bromide peptides (33).…”

mentioning

confidence: 99%

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Characterization of the proteins of intracisternal type A and extracellular oncornavirus-like particles produced by MOPC-460 myeloma cells

Robertson¹,

Jhabvala²,

Godefroy-Colburn³

et al. 1979

J Virol

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The mouse plasmacytoma cell line, MOPC-460, produces both intracisternal and intracytoplasmic A-type particles when grown as a solid tumor. When these cells are grown either as an ascites tumor or in tissue culture, a third type of particle is produced extracellularly. This particle, the "myeloma-associated virus," is closely related to, and probably an alternate form of, the intracisternal A-type particle. The proteins present in these two types of particles were compared by tryptic peptide mapping. Both types of particles were found to contain essentially the same major proteins of 76,000 (p76), 68,000 to 70,000 (p68-70), and 45,000 (p45) daltons, in addition to varying amounts of smaller proteins. The relative proportions of all these proteins varied from preparation to preparation in an

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Section: Resultsmentioning

confidence: 99%

“…Gradients were fractionated by puncturing the tubes, and samples were frozen. Intracisternal A particles were prepared from solid tumors as described previously (33).…”

Section: Materials and Metmodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Characterization of the proteins of intracisternal type A and extracellular oncornavirus-like particles produced by MOPC-460 myeloma cells

Robertson¹,

Jhabvala²,

Godefroy-Colburn³

et al. 1979

J Virol

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“…Many of the murine plasmacytomas so far examined have been reported to produce type C particles (6,7,15,(19)(20)(21)23). Since in some murine myeloma variants a correlation was seen between the loss of type C particles and a halt in the production of immunoglobulin (16,17), a direct relationship between these two activities was suggested (4,5,21).…”

mentioning

confidence: 99%

Type C virus and immunoglobulin A production by murine myeloma MOPC-315: two independent activities

Gazit¹,

Yaniv²,

Halperin³

et al. 1979

Infect Immun

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The suspected correlation between cessation of type C virus production and halt in immunoglobulin secretion by murine myeloma cells was studied. Employing two variants of the murine myeloma MOPC-315, immunoglobulin A-producing and nonproducing cells, we demonstrated that the two myelomas release similar levels of type C viruses which share common nucleotide sequences and that the viral genomes are equally expressed within the cells. Thus, the suggested relation between these two activities does not apply for MOPC-315 cells and probably for other murine myelomas also.

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Feline malignant mammary tumors. III. Presence of C‐particles and intracisternal A‐particles and their relationship with feline leukemia virus antigens and RD‐114 virus antigens

Calafat

Weijer

Daams

1977

Intl Journal of Cancer

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Thirty-six feline mammary tumors were examined by the electron microscope, and by the indirect immunofluorescence (IFA) test with anti-FeLV and anti-RD-114 serum. In 11 (30.6%) tumors intracisternal A-particles (IAP) were found. One of these tumors contained a few particles with an electron-dense nucleoid in the cisternae of the endoplasmic reticulum. In 7 (19.4%) other tumors C-particles were found and in the remaining 18 (50.0%) no particles at all could be detected. In 11 (30.6%) tumors FeLV antigens and in 20 (55.5%) tumors RD-114 virus antigens were present. In 9 (25%) tumors we found a high (greater than 1/64) and in 11 (30.6%) tumors a low (greater than 1/16 less than or equal to 1/64) titer. There was a good correlation between the presence of C-particles and the demonstration of FeLV-antigen but none between IAP and FeLV antigens. No correlation was found between RD-114 virus antigens and any type of particle. Morphologically, the IAP found in feline mammary tumors were indistinguishable from the IAP present in mammary tumors of some inbred mice strains. The IAP in feline mammary tumors possibly represent an endogenous virus, different from RD-114 virus. The role of these viruses in the etiology of feline mammary tumors is discussed.

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Relationships between intracisternal type A and extracellular oncornavirus-like particles produced in murine MOPC-460 myeloma cells

Cited by 25 publications

References 38 publications

Characterization of the proteins of intracisternal type A and extracellular oncornavirus-like particles produced by MOPC-460 myeloma cells

Characterization of the proteins of intracisternal type A and extracellular oncornavirus-like particles produced by MOPC-460 myeloma cells

Type C virus and immunoglobulin A production by murine myeloma MOPC-315: two independent activities

Feline malignant mammary tumors. III. Presence of C‐particles and intracisternal A‐particles and their relationship with feline leukemia virus antigens and RD‐114 virus antigens

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