Relationships between peroxidases and budding in date palm tissues cultured in vitro

Abstract

In natural conditions, a cyclic growth of the apical meristem of the date palm is observed. This type of growth is also encountered in the behavior of vegetative shoots in in vitro culture, a growth stage succeeding a budding stage. Peroxidase activity is related to a cyclic budding phenomenon, where maximum enzyme activity and also a modification of isoperoxidase content are noted at the same time as the formation of buds.

Relationships between peroxidases and in vitro bulbification in garlic (Allium sativum L.)

“…The maximum peak of activity that was observed at 10 d ( Fig. 1) would reflect the morphogenic changes of bud formation that always occurred prior to bulbification initiation, since de novo vegetative bud formation always occurs after previous increase of the peroxidase activity in the explant, as was reported in tobacco (Thorpe and Gaspar, 1978), sugar beet (Kevers et al, 1981), and date palm (Booij et al, 1993). Together with this process there would be a possible increase in the IAA levels, that would lead to bud formation and growth.…”

Identification de la variabilité intraclonale des vitroplants de palmier dattier issus de culture in vitro par organogenèse : étude morphologique

“…Les variations morphologiques révélées chez les vitroplants de palmier dattier suggèrent l'influence de la technique de culture in vitro utilisée. En effet, plusieurs facteurs, sources de variations somaclonales, sont liés à la technique de culture des tissus de palmier dattier, à savoir, entre autres : la durée de la culture in vitro et, plus précisément, la longueur de la phase non morphogène [27,28], les désordres physiologiques dus aux substances de croissance mutagènes [29,30] et l'origine de l'explant mis en culture [31]. Ces aspects rentrent dans ce qu'on peut appeler la maîtrise de la technique de culture in vitro du palmier dattier.…”