Relationships between thyroid status, tissue oxidative metabolism, and muscle differentiation in bovine fetuses

Cassar-Malek, Isabelle; Picard, Brigitte; Kahl, Stanislaw; Hocquette, Jean-François

doi:10.1016/j.domaniend.2006.04.011

Cited by 24 publications

(14 citation statements)

References 43 publications

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“…The second principal and subsequent components describe the maximum amount of remaining variability and must be independent of (orthogonal to) the first principal component. The main steps in PCA have been described for previous applications in cattle [35,36]. Results are presented in a 2D projection graph where variables near each other at the periphery of the circle are positively correlated, orthogonal variables are independent and variables separated by 180° are negatively correlated.…”

Section: Methodsmentioning

confidence: 99%

Specific fibre composition and metabolism of the rectus abdominis muscle of bovine Charolais cattle

et al. 2010

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BackgroundAn important variability of contractile and metabolic properties between muscles has been highlighted. In the literature, the majority of studies on beef sensorial quality concerns M. longissimus thoracis. M. rectus abdominis (RA) is easy to sample without huge carcass depreciation and may appear as an alternative to M. longissimus thoracis for fast and routine physicochemical analysis. It was considered interesting to assess the muscle fibres of M. rectus abdominis in comparison with M. longissimus thoracis (LT) and M. triceps brachii (TB) on the basis of metabolic and contractile properties, area and myosin heavy chain isoforms (MyHC) proportions. Immuno-histochemical, histochemical, histological and enzymological techniques were used. This research concerned two populations of Charolais cattle: RA was compared to TB in a population of 19 steers while RA was compared to LT in a population of 153 heifers.ResultsRA muscle had higher mean fibre areas (3350 μm2 vs 2142 to 2639 μm2) than the two other muscles. In RA muscle, the slow-oxidative fibres were the largest (3957 μm2) and the fast-glycolytic the smallest (2868 μm2). The reverse was observed in TB muscle (1725 and 2436 μm2 respectively). In RA muscle, the distinction between fast-oxidative-glycolytic and fast-glycolytic fibres appeared difficult or impossible to establish, unlike in the other muscles. Consequently the classification based on ATPase and SDH activities seemed inappropriate, since the FOG fibres presented rather low SDH activity in this muscle in comparison to the other muscles of the carcass. RA muscle had a higher proportion of I fibres than TB and LT muscles, balanced by a lower proportion either of IIX fibres (in comparison to TB muscle) or of IIA fibres (in comparison to LT muscle). However, both oxidative and glycolytic enzyme activities were lower in RA than in TB muscle, although the LDH/ICDH ratio was higher in RA muscle (522 vs 340). Oxidative enzyme activities were higher in RA than in LT muscle, whereas glycolytic enzyme activity was lower. In RA muscle, contractile and metabolic properties appeared to be less well-correlated than in the two other muscles.ConclusionsRA muscle has some particularities in comparison to the LT and TB muscles, especially concerning the unusual large cross-section surface of SO fibres and the very low oxidative activity of intermediate IIA fibres.

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Section: Methodsmentioning

confidence: 99%

Specific fibre composition and metabolism of the rectus abdominis muscle of bovine Charolais cattle

et al. 2010

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“…Maximum activity levels of the enzymes reflecting the glycolytic metabolic pathway [lactate dehydrogenase (LDH), EC 1.1.1.27; phosphofructokinase, (PFK), EC 2.7.1.11], mitochondrial density [isocitrate dehydrogenase (ICDH), EC 1.1.1.42; citrate synthase (CS), EC 4.1.3.7], oxidative phosphorylation cytochrome-c oxidase (COX), EC 1.9.3.1] were measured spectrophotometrically as previously described (Cassar-Malek et al, 2007;Jurie et al, 2006). Briefly, muscle samples were homogenized with a polytron in a 5% (wt=vol) solution with 10 mM Tris (pH 8.0), sucrose 0.25 M and 2 mM EDTA.…”

Section: Metabolic Enzyme Activitiesmentioning

confidence: 99%

Myogenesis Is Delayed in Bovine Fetal Clones

Cassar-Malek

Picard

Jurie

et al. 2010

Cellular Reprogramming

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We have recently reported that maturation of the skeletal muscle is delayed in cloned calves during their first year postnatally. This delay could originate from perturbations in fetal myogenesis. The aim of this study was to evaluate the developmental characteristics of muscle in clones versus animals derived from conventional reproduction. We have characterized the anatomical and biochemical properties of the Semitendinosus muscle of clones versus controls at day 60 and day 260. We have analyzed the contractile and metabolic properties of muscle fibers by measuring the abundance of myosin heavy chain (MyHC) isoforms and activities of metabolic enzymes (LDH, PFK, COX, CS, ICDH), respectively. The spatial repartition of some components of the extracellular matrix (collagen types I, IV, VI, chondroitin-6-sulfate, decorin, and tenascin-X) was also studied. At day 60 we found lower numbers and structural organization of fibers, and a delay in the setup of the extracellular matrix. IGF-2 transcript abundance was lower in clones than in their controls. There was no difference in the expression of VEGF (a growth factor regulating vascularization and myogenesis) and its receptor. At day 260 the muscles of fetal clones have not reached the same degree of differentiation than controls as shown by their lower energy metabolisms and their MyHC pattern. These results show for the first time that disturbances in myogenesis occur early in fetal life in cloned cattle.

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“…Thyroid hormones have many target tissues; they affect the metabolism of various components and play a crucial role in regulating primary and basal metabolism (Thrall et al 2004;Chatterjea and Shinde 2005;Cassar-Malek et al 2007;Kale et al 2007;Todini 2007). Overall, these hormones stimulate protein synthesis and increase blood sugar and lipid turnover (Chatterjea and Shinde 2005;Todini 2007).…”

Section: Introductionmentioning

confidence: 95%

Relationship between plasma thyroid hormones and some biochemical parameters in Iranian Sarabi calves

et al. 2010

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The thyroid gland has some important endocrine hormones that regulate basal metabolism in various tissues of domestic animals. Thyroid hormones have a central role in animals' development and their tissue functions. In this study, the relationship between the plasma thyroxine (T 4 ), triiodothyronine (T 3 ), free thyroxine (fT 4 ), free triiodothyronine (fT 3 ), triglyceride, cholesterol, glucose, total protein, and albumin concentrations as well as albumin/globulin ratio in different ages of Iranian Sarabi calves was investigated. Blood samples were collected from the jugular vein of 47 clinically healthy calves free from internal and external parasites (grouped according to their age-1-14 days, 1-2, and 3-6 months) in early of winter. The level of thyroid hormones was determined by chemiluminescence, and other parameters were measured by spectrophotometry using commercial kits. Our data from this study indicates that there was no significant difference and correlation in all the studied parameters between age groups and sexes. But we found a significant correlation between plasma T 4 and total protein (P<0.05, r=0.600), T 4 and albumin (P<0.05, r=0.575), T 3 and fT 3 (P<0.05, r= 0.610), T 3 and total protein (P<0.01, r=0.725), T 3 and glucose (P<0.01, r=0.685), and fT 4 and fT 3 (P<0.05, r= 0.609) concentrations as well as between total protein and albumin/globulin ratio (P<0.01, r=−0.783).

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Relationships between thyroid status, tissue oxidative metabolism, and muscle differentiation in bovine fetuses

Cited by 24 publications

References 43 publications

Specific fibre composition and metabolism of the rectus abdominis muscle of bovine Charolais cattle

Specific fibre composition and metabolism of the rectus abdominis muscle of bovine Charolais cattle

Myogenesis Is Delayed in Bovine Fetal Clones

Relationship between plasma thyroid hormones and some biochemical parameters in Iranian Sarabi calves

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