Relationships of bovine ephemeral fever epizootics to population immunity and virus variation

Ting, Lu‐Jen; Lee, Ming-Shiuh; Lee, Shu-Hwae; Tsai, Hsiang‐Jung; Lee, Fan

doi:10.1016/j.vetmic.2014.07.021

Cited by 29 publications

(25 citation statements)

References 19 publications

(16 reference statements)

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“…In addition, the average number of seroconversions had a strong geographical component that reflects patterns of vector biology responsible for BEF virus transmission [23]. Furthermore, the lower neutralizing antibody titers in 2007 suggesting reduced immunity reflects the importance of population immunity for the control of BEFV [24]. On the other hand, there was no effect of region on the seroprevalence and mortality rate of the disease which disagrees with the findings of Zaghawa et al, 2015 in the same regions.…”

Section: Discussionmentioning

confidence: 75%

Bovine ephemeral fever epidemics in Kingdom Saudi Arabia: clinical, epidemiological and molecular investigation

Zaghawa

Housawi

Alnaeem

et al. 2017

J Infect Dev Ctries

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Introduction: Bovine ephemeral fever virus (BEFV) is an arthropod borne Rhabdovirus affects cattle and water buffalo causes acute febrile disease. Methodology: The clinical picture and epidemiological pattern of BEF were described among cattle in epidemics of 2007, 2009 and 2011 in four geographical regions of Kingdom Saudi Arabia (Eastern, Jizan, Qasim, and Riyadh). Serum samples were tested using VNT. Virus isolation and molecular characterization were carried out for the first time in KSA. Results: The main clinical symptoms were fever, stiffness, lameness, salivation and subcutaneous emphysema. The prevalence and the mortality rate of BEF have decreased from 70% and 4.6% in 2007 to 30% and 0.6% in 2011, respectively in the 4 studied areas. There was no region association with higher prevalence of BEF. The intracluster correlation (ICC) was estimated for the first time in KSA as 0.0034. BEFV had been isolated from 11 out of 20 samples (55%) and isolation was confirmed by VNT. The molecular detection of BEFV by RT-PCR and realtime RT-qPCR were found more sensitive for diagnosis of the disease than virus isolation; 80% and 90% for the former tests and 55% for the latter. Three isolates were sequenced, they showed 84.7% -100% identities in between and shared 90.4%-96.5% sequence identity with a previously published sequence from Australia (KF679404). The generated sequences belonged to 3 rd cluster of BEFV glycoprotein. Conclusions: BEF occurrence has cyclic nature and the efficacy of vaccines prepared from local strains has to be evaluated and considered in diseases control.

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Section: Discussionmentioning

confidence: 75%

Bovine ephemeral fever epidemics in Kingdom Saudi Arabia: clinical, epidemiological and molecular investigation

Zaghawa

Housawi

Alnaeem

et al. 2017

J Infect Dev Ctries

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“…cluster as Japanese and Chinese isolates and were genetically distant from Australian and Israel isolates (11,31). Antigenic variations among these strains or even between field and vaccine strains occurred as amino acid substitutions were found on the epitopes of G proteins (11,32).…”

Section: Discussionmentioning

confidence: 97%

Immunogenicity of transmembrane deleted-G protein of bovine ephemeral fever virus (BEFV) expressed in insect cells

2018

Turk J Vet Anim Sci

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Bovine ephemeral fever (BEF) is a common febrile disease in cattle in tropical countries causing loss of milk production and reproductive performance. Recently, severe outbreaks have occurred in many countries in East Asia, the Middle East, and Southeast Asia, especially in the rainy season, while limited vaccines and diagnostic tools are available. Thus, the aim of this study was to produce a secreted, soluble form of BEFV G glycoprotein in insect cells for further applications. BEFV G gene expressing transmembrane-deleted G glycoprotein (G∆TM) was constructed and cloned into the baculovirus vector. The recombinant G∆TM protein was expressed in High5 insect cells and then purified by affinity chromatography. The purified G∆TM reacted specifically with a convalescent bovine serum. Four 8-week-old Wistar rats were injected subcutaneously with the purified G∆TM protein. Postimmunized rat serum samples also strongly reacted with the G protein by western blot and immunoperoxidase mono player assay (IPMA). These results indicated the potential use of the transmembrane-deleted G protein to develop protein based-diagnostic tools for BEFV control programs.

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“…recently, it has been reported that 2 of 3 major epizootics and 87% of regional outbreaks in taiwan, where inactivated BeF vaccines have been applied, were associated with lower neutralizing antibody titers and immunity coverage (tING et al, 2014). Otherwise, the data on the efficacy of commercial vaccines in various formulations for the prevention of infection are not exactly clear (WaLker and kLeMeNt, 2015).…”

Section: Discussionmentioning

confidence: 99%

Assessment of the molecular epidemiology of bovine ephemeral fever in Turkey

et al. 2017

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Relationships of bovine ephemeral fever epizootics to population immunity and virus variation

Cited by 29 publications

References 19 publications

Bovine ephemeral fever epidemics in Kingdom Saudi Arabia: clinical, epidemiological and molecular investigation

Bovine ephemeral fever epidemics in Kingdom Saudi Arabia: clinical, epidemiological and molecular investigation

Immunogenicity of transmembrane deleted-G protein of bovine ephemeral fever virus (BEFV) expressed in insect cells

Assessment of the molecular epidemiology of bovine ephemeral fever in Turkey

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