Relative abundance of G protein-coupled receptor 30 and localization in testis and epididymis of sheep at different developmental stages

Lu, Peiyao; Wang, Fuchuan; Song, Xianyi; Liu, Yang; Zhang, Kai; Cao, Ningxian

doi:10.1016/j.anireprosci.2016.10.002

Cited by 4 publications

(5 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The expression of ERs has been widely documented in the epididymis of several species (mice, rats, dogs, cats, and monkeys) [ 32 , 35 , 36 , 46 , 58 ] with a common observation of the presence of ER-alpha in the efferent ductules that not only provide to the reabsorption of more than 90% of the rete testis fluid but concentrate sperm for epididymal storage [ 59 ]. In addition, the very wide GPER expression in the epididymis of pigs [ 28 , 60 ], rats [ 30 ], sheep [ 61 ] and in humans’ ductuli efferents and proximal epididymis [ 29 ] suggests its involvement in sperm maturation, protection, and storage. However, GPER could play other different roles such as in the regulation of contractility, demonstrated by its presence in the muscle cells of the human ductuli efferent and proximal epididymis [ 29 , 62 ].…”

Section: Discussionmentioning

confidence: 99%

G Protein-Coupled Estrogen Receptor (GPER) and ERs Are Modulated in the Testis–Epididymal Complex in the Normal and Cryptorchid Dog

Liguori,

Tafuri,

Pelagalli

et al. 2024

Veterinary Sciences

View full text Add to dashboard Cite

There is growing evidence by the literature that the unbalance between androgens and estrogens is a relevant condition associated with a common canine reproductive disorder known as cryptorchidism. The role of estrogens in regulating testicular cell function and reproductive events is supposedly due to the wide expression of two nuclear estrogen receptors (ERs), ER-alpha and ER-beta and a trans-membrane G protein-coupled estrogen receptor (GPER) in the testis. In this study, immunohistochemistry, Western blotting and qRT-PCR were used to assess the distribution and expression of GPER in the testis–epididymal complex in the normal and cryptorchid dog. ER-alpha and ER-beta were also evaluated to better characterize the relative abundances of all three receptors. In addition, in these tissues, the expression level of two proteins as SOD1 and Nrf2 normally associated with oxidative stress was investigated to evaluate a possible relationship with ERs. Our data revealed changes in the distribution and expression of the GPER between the normal and cryptorchid dog. In particular, dogs affected by cryptorchidism showed an upregulation of GPER at level of the examined reproductive tract. Also considering the obtained result of a modulation of SOD1 and Nrf2 expression, we could hypothesize the involvement of GPER in the cryptorchid condition. Further studies are, however, necessary to characterize the role of GPER and its specific signaling mechanisms.

show abstract

Section: Discussionmentioning

confidence: 99%

G Protein-Coupled Estrogen Receptor (GPER) and ERs Are Modulated in the Testis–Epididymal Complex in the Normal and Cryptorchid Dog

Liguori,

Tafuri,

Pelagalli

et al. 2024

Veterinary Sciences

View full text Add to dashboard Cite

show abstract

“…GPER has been detected in many male reproductive structures, such as the testes (Cassault-Meyer et al , 2014;Gautier et al , 2016;Lucas et al , 2010), spermatozoa (Arkoun et al , 2014;Cassault-Meyer et al , 2014;Gautier et al , 2016), and prostate (Rago et al , 2016). It has also been found in the efferent ductules and epididymis (Cao et al , 2017;Hess et al , 2011;Katleba et al , 2015;Krejcirova et al , 2018;Lu et al , 2016;Malivindi et al , 2018;Martinez-Traverso et al , 2015;Menad et al , 2017;Pereira et al , 2014;Rago et al , 2018), indicating that GPER may play important roles in sperm maturation, protection and storage (Table 1). For instance, in the corpus epididymis of postnatal pigs, GPER participates in sperm maturation by affecting the formation of the blood-epididymal barrier (Katlebaet al , 2015).…”

Section: Endogenous Angiotensin System and Agtr2 In Epididymismentioning

confidence: 98%

“…In addition, the relative abundance of GPER in the efferent ductules and each part of the epididymis, the cellular localization of GPER, and the molecular weight of the protein differ depending on the species, developmental stage, and physiological cycle studied (Krege et al , 1995;Krejcirova et al , 2018;Lu et al , 2016;Pereira et al , 2014). Therefore, the role of GPER in the efferent ductules and epididymis appears to be complex.…”

Section: Endogenous Angiotensin System and Agtr2 In Epididymismentioning

confidence: 99%

Function and therapeutic potential of GPCRs in epididymis

Zhang¹,

Wang²,

Lin³

et al. 2020

Preprint

View full text Add to dashboard Cite

Infertility rates for both females and males have increased continuously in recent years. Currently, effective treatments for male infertility with defined mechanisms or targets are still lacking. G protein-coupled receptors (GPCRs) are the largest class of drug targets, but their functions and the implications on therapeutic development for male infertility largely remain elusive. Nevertheless, recent studies have shown that several members of the GPCR superfamily play crucial roles in the maintenance of ion-water homeostasis of the epididymis, development of the efferent ductules, formation of the blood-epididymal barrier, and maturation of sperm. Knowledge of the functions, genetic variations, and working mechanisms of such GPCRs, along with the drugs and ligands relevant to their specific functions, provide future directions and elicit great arsenal for potential therapy development for treating male infertility.

show abstract

“…GPER has been detected in many male reproductive structures, such as the testes (Cassault-Meyer, Gress, Seralini, & Galeraud-Denis, 2014;Gautier et al, 2016;Lucas et al, 2010), spermatozoa (Arkoun et al, 2014;Cassault-Meyer et al, 2014;Gautier et al, 2016), and prostate (Rago, Romeo, Giordano, Ferraro, & Carpino, 2016). It has also been found in the efferent ductules and epididymis (Cao et al, 2017;Hess et al, 2011;Katleba et al, 2015;Krejcirova et al, 2018;Lu et al, 2016;Malivindi, Aquila, & Rago, 2018;Martinez-Traverso & Pearl, 2015;Menad et al, 2017;Pereira et al, 2014;Rago et al, 2018), indicating that GPER may play important roles in sperm maturation, protection and storage (Table 1). For instance, in the corpus epididymis of postnatal pigs, GPER participates in sperm maturation by affecting the formation of the blood-epididymal barrier (Katleba et al, 2015).…”

Section: Complex Functions Of Gper In the Epididymismentioning

confidence: 99%

“…In addition, the relative abundance of GPER in the efferent ductules and each part of the epididymis, the cellular localization of GPER and the molecular weight of the protein differ depending on the species, developmental stage and physiological cycle studied (Krege et al, 1995;Krejcirova et al, 2018;Lu et al, 2016;Pereira et al, 2014). Therefore, the role of GPER in the efferent ductules and epididymis appears to be complex.…”

Section: Complex Functions Of Gper In the Epididymismentioning

confidence: 99%

Function and therapeutic potential of G protein‐coupled receptors in epididymis

Zhang

Wang

Lin

et al. 2020

British J Pharmacology

View full text Add to dashboard Cite

Infertility rates for both females and males have increased continuously in recent years. Currently, effective treatments for male infertility with defined mechanisms or targets are still lacking. G protein-coupled receptors (GPCRs) are the largest class of drug targets, but their functions and the implications for the therapeutic development for male infertility largely remain elusive. Nevertheless, recent studies have shown that several members of the GPCR superfamily play crucial roles in the maintenance of ion-water homeostasis of the epididymis, development of the efferent ductules, formation of the blood-epididymal barrier and maturation of sperm. Knowledge of the functions, genetic variations and working mechanisms of such GPCRs, along with the drugs and ligands relevant to their specific functions, provide future directions and a great arsenal for new developments in the treatment of male infertility.

show abstract

Relative abundance of G protein-coupled receptor 30 and localization in testis and epididymis of sheep at different developmental stages

Cited by 4 publications

References 24 publications

G Protein-Coupled Estrogen Receptor (GPER) and ERs Are Modulated in the Testis–Epididymal Complex in the Normal and Cryptorchid Dog

G Protein-Coupled Estrogen Receptor (GPER) and ERs Are Modulated in the Testis–Epididymal Complex in the Normal and Cryptorchid Dog

Function and therapeutic potential of GPCRs in epididymis

Function and therapeutic potential of G protein‐coupled receptors in epididymis

Contact Info

Product

Resources

About