Relative bioavailability of 13C5-folic acid in pectin-coated folate fortified rice in humans using stable isotope techniques

Ambrosis, A de; Vishnumohan, Shyamala; Paterson, Janet; Haber, Paul; Arcot, Jayashree

doi:10.1038/ejcn.2016.122

Cited by 6 publications

(4 citation statements)

References 25 publications

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“…In this view, the application of stable isotope dilution assay (SIDA) has major advantages including the complete compensation for losses of analytes during extraction, for ion suppression during LC-MS/MS, and the so-called carrier effect (Rychlik and Asam, 2009 ). SIDA has proven its superiority during the last years and many research groups are using this state of the art methodology for folate analysis (Pawlosky et al, 2003 ; Pfeiffer et al, 2004 ; Ringling and Rychlik, 2013 ; de Ambrosis et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Improved Stable Isotope Dilution Assay for Dietary Folates Using LC-MS/MS and Its Application to Strawberries

et al. 2018

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Folates play an important role in the human body and a deficiency of this vitamin can cause several diseases. Therefore, a reliable analytical method is crucial for the determination of folate vitamers in strawberries and other dietary folate sources. A stable isotope dilution LC-MS/MS method for analyzing folates in food was developed and validated. The folate vitamers Pteroylmonoglutamic acid, tetrahydrofolate, 5-methyltetrahydrofolate, and 5-formyltetrahydrofolate were quantified using 13C-labeled internal standards. Validation of the assay was accomplished by determining linearity, precision, recovery, limit of detection, and limit of quantification and revealed to be a precise, sensitive, and accurate method to determine folate vitamers. Strawberries are worldwide consumed and known to be a good dietary source of nutritive compounds. Using this method, folate concentrations in selected commercial strawberry cultivars and experimental breeding lines grown in Germany and Australia were investigated. Total folates varied from 59 to 153 μg/100 g on fresh weight basis. Furthermore, folate content after lyophilizing or freezing did not show any significant differences compared to fresh strawberries. However, significant losses of total folates in pureed strawberries could be observed after 5 days of storage with only 16% of the original concentration retained. In summary, some of the investigated strawberry cultivars/breeding lines can be considered as rich dietary sources of natural folates.

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Section: Introductionmentioning

confidence: 99%

Improved Stable Isotope Dilution Assay for Dietary Folates Using LC-MS/MS and Its Application to Strawberries

et al. 2018

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“…To ensure that the diet plan did not result in an increase of plasma folate concentration, the performance of a folate-free control day was crucial for determination of the relative bioavailability. However, recent studies have often used only the first blood sample drawn prior to consumption of the test food as a control [36].…”

Section: Discussionmentioning

confidence: 99%

Quantitation of 5-methyltetraydrofolic acid in plasma for determination of folate status and clinical studies by stable isotope dilution assays

et al. 2019

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Folates play a key role in the prevention of neural tube defects in newborns. Thus, it is important to reliably determine the bioavailability of folates from various foods. Accurate analytical methods are essential for quantifying blood-folates, especially in human studies. Here, we present the development and validation of a sensitive method using stable isotope dilution liquid chromatography coupled with mass spectrometry for determining various folates in plasma. Moreover, this study reports the applicability of the developed method to a human pilot study using strawberries as a test food. Validation of the assay revealed the precision, sensitivity, and accuracy of the method in determining the predominant 5-methyltetrahydrofolate in plasma. This method was also applicable for the screening of individual folate status using finger prick blood and for monitoring the post-absorptive plasma-concentration curve. Moreover, the human study revealed a high recovery of strawberry folates with a calculated relative bioavailability of 96.2%. Thus, the developed method enables prospective bioavailability studies. This work also confirmed, via human studies, that strawberries are a rich and natural source of folates that are available for human metabolism.

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“…This chemoenzymatic pterin synthesis can be integrated into other enzymatic procedures to generate folate derivatives , and other high-value natural products that are not easily accessible by conventional synthesis . It can be applied to nutritional, medical, and cell-biological research to address questions of in vivo bioavailability and to explore the kinetics of folate metabolism in intact cells and organisms. ,− …”

Section: Discussionmentioning

confidence: 99%

Chemoenzymatic Assembly of Isotopically Labeled Folates

et al. 2017

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Pterin-containing natural products have diverse functions in life, but an efficient and easy scheme for their in vitro synthesis is not available. Here we report a chemoenzymatic 14-step, one-pot synthesis that can be used to generate C- andN-labeled dihydrofolates (HF) from glucose, guanine, and p-aminobenzoyl-l-glutamic acid. This synthesis stands out from previous approaches to produce HF in that the average yield of each step is >91% and it requires only a single purification step. The use of a one-pot reaction allowed us to overcome potential problems with individual steps during the synthesis. The availability of labeled dihydrofolates allowed the measurement of heavy-atom isotope effects for the reaction catalyzed by the drug target dihydrofolate reductase and established that protonation at N5 of HF and hydride transfer to C6 occur in a stepwise mechanism. This chemoenzymatic pterin synthesis can be applied to the efficient production of other folates and a range of other natural compounds with applications in nutritional, medical, and cell-biological research.

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Relative bioavailability of 13C5-folic acid in pectin-coated folate fortified rice in humans using stable isotope techniques

Abstract: This study is the first demonstration of the bioavailability of folate in pectin-coated fortified rice in humans.

Cited by 6 publications

References 25 publications

Improved Stable Isotope Dilution Assay for Dietary Folates Using LC-MS/MS and Its Application to Strawberries

Improved Stable Isotope Dilution Assay for Dietary Folates Using LC-MS/MS and Its Application to Strawberries

Quantitation of 5-methyltetraydrofolic acid in plasma for determination of folate status and clinical studies by stable isotope dilution assays

Chemoenzymatic Assembly of Isotopically Labeled Folates

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