Currently, various Nickel-Titanium rotary files are used in endodontic treatment, but there is no one perfect system that can be applied to any clinical situation. Therefore, the combined uses of various file systems which can emphasize the advantages of each system are introduced as hybrid instrumentation.The ProTaper system is efficient in body shaping and apical pre-enlargement but is reported to have more possibility of transportation and produce more aberrations and deformation in more or less severe curved canals. Recently, new ProTaper system (ProTaper Universal) with different configuration and cross-sectional design to overcome the week points of ProTaper have been marketed.The purpose of this study was to compare and evaluate the shaping abilities of ProTaper, ProTaper Universal system, and two hybrid methods using S-series of ProTaper Universal and Hero Shaper or ProFile.The time lapses for instrumentation were measured and the used files were inspected for distortion. The pre-and post-instrumented root canals were scanned and superimposed to evaluate the aberrations and reduction of root canal curvature and change of radius of canal curvature. The increased canal width and apical centering ratio were calculated at 1, 2, 3, 4 and 5 ㎜ levels from apical foramen.Under the conditions of this study, the ProTaper Universal seems to have better shaping ability than ProTaper in terms of instrumented width and instrumentation time. It may be suggested that the ProTaper Universal system is efficient as much as hybrid instrumentation using ProTaper and other constant-tapered NiTi file systems in highly experienced operators. . And the use of NiTi rotary instrumentation can effectively produce a well-tapered root canal form sufficient for obturation with minimal risk of transporting the original canal, and are helpful in the preparation of curved root canals [2][3][4][5][6] . It is estimated that there are more than least 18 different rotary file brands in the endodontic marketplace 7) . Most of these NiTi file systemse.g. ProFile (Dentsply Maillefer, Ballaigues, Switzerland), K3 (SybronEndo, Glendora, France), Mtwo (VDW, Munich, Germany), Hero642 (Micromega, Besancon, France) and Hero Shaper (Micromega, Besancon, France), -have a constant tapered shaft design, while these have their own rake angles and radial lands 6,[8][9][10] . Distinctively, the ProTaper system (Dentsply Maillefer, Ballaigues, Switzerland) is an unique instrument which has progressively changing tapered shaft design along a single instrument in the shaft design 11,12) . And the design of cross-section of ProTaper -convex triangular -is claimed to reduce the contact area between the file and dentin, and to have excellent cutting efficiency 12) . It was claimed that the ProTaper system provides a continuous tapered preparation of the root canal, without significant transportation of the original curvature of the root canal 12) . And also it was suggested that the ProTaper instruments might be better suited for curved and constricted canals tha...