Relative Frequency of Cytomegalovirus (CMV) in Tissue Samples of Women with Breast Cancer in Sanandaj, Iran.

Karimi, Maryam; Hosseini, Seyedeh Zeinab; Nikkhoo, Bahram; Mohammadi, Farid Soleimani

doi:10.21746/ijbio.2016.03.0014

Cited by 3 publications

(3 citation statements)

References 25 publications

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“…On the other hand, among the 35 control group samples (fibroadenoma), 10 (28.57%) cases were positive for HCMV DNA (P >0.028.). In accordance to our findings, (Karimi et al, 2016) detected HCMV DNA in 26/50 (58%) samples of invasive breast carcinoma by using the nested-PCR method in Sanandaj city, Iran. Taher et al, (2013) from Sweden demonstrated HCMV IE protein expression in 100% of 73 breast cancer samples using the IHC method and real-time PCR.…”

Section: Resultssupporting

confidence: 90%

Human Cytomegalovirus DNA among Women with Breast Cancer

Sepahvand

Makvandi

Samarbafzadeh

et al. 2019

Asian Pac J Cancer Prev

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Section TitleBreast cancer is the most common cause of death among women worldwide. Although there are many known risk factors in breast cancer development, infectious diseases have appeared as one of the important key to contribute to carcinogenesis formation. The effects of Human Cytomegalovirus (HCMV) on women with breast cancer has been recently studied and reported. To contribute to this research trend, this study was conducted to evaluate the association between HCMV and the women with breast cancer.Objective:This experiment aimed to evaluate HCMV DNA in women with breast cancer in Ahvaz city, Iran. Materials and Methods:A total of 37 formalin fixed paraffin embedded tissues of the patients with ductal breast carcinoma and 35 paraffin embedded tissues of the patients with fibro adenoma as control group were collected. The deparaffinization of all the samples were carried out and the DNA was extracted. Initially, the PCR test was carried out to detect beta –globulin DNA as an internal control. For those samples positive for beta –globulin DNA, Polymerase Chain reaction (PCR) was used to detect HCMV for the tests and control samples. Results:Among 37 ductal breast carcinoma, 20 (54.04%) cases were proved positive for HCMV DNA by PCR. While among the 35 control group (fibroadenoma), 10 (28.57%) cases were positive for HCMV DNA (P >0.028). The prevalences of HCMV DNA among the age groups 30-39, 40-49 and >50 years were 7 (72.22%), 9 (69.23%), 4 (57.14%), respectively (P=0.066). A high frequency of HCMV DNA was detected in tumor grade III, 13/18 (58.33%) compared with tumor grade II, 7/19 (36.84%) (p=0.044). A high frequency of 16/24 (66.66%) of HCMV DNA was found in invasive ductal breast cancer compared with 4/13 (30.76%) HCMV DNA in situ (P<0.028). Conclusion:A high prevalence of 54.05% HCMV was found among the patients with ductal carcinoma. The percentages of the high prevalence of HCMV among age group (40-49) years, tumors grades, and invasive stage were (69.23%), (58.33%), (66.66%), respectively. Further study of HCMV in the latency phase in patients with ductal carcinoma would be necessary to extend our knowledge.

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Section: Resultssupporting

confidence: 90%

Human Cytomegalovirus DNA among Women with Breast Cancer

Sepahvand

Makvandi

Samarbafzadeh

et al. 2019

Asian Pac J Cancer Prev

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“…[ 21 ] also reported a significant association between HCMV and breast cancer, with higher serum levels of HCMV IgG in 82% of 28 patients with inflammatory breast carcinoma compared to 65% of 49 patients with non-inflammatory breast carcinoma. Karimi et al [ 22 ] from Iran also detected HCMV DNA in 26 of the 50 samples (58%) of invasive breast carcinoma by using the nested-PCR method in Sanandaj city, Kurdistan Province. Perhaps the different prevalence of HCMV in various geographical areas in Iran may justify the different findings of the Karimi et al study and ours.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of human cytomegalovirus antigen expression in invasive breast carcinoma in a population of Iranian patients

Mohammadizadeh

Mahmudi

2017

Infect Agents Cancer

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BackgroundThe role of human cytomegalovirus (HCMV) in the development of breast carcinoma is questionable. The aim of this study was to evaluate the expression of the immediate early antigen (IE) of HCMV in breast carcinoma and its association with some clinicopathologic factors in a population of Iranian patients.MethodsFormalin-fixed and paraffin-embedded tissue blocks from the pathology laboratories of the Azahra and Shahid Beheshti hospitals, Isfahan, Iran, from 2013 to 2016, were used in the study. We used immunohistochemistry and real-time PCR to detect the IE-antigen of HCMV in breast carcinoma, normal tissue adjacent to carcinoma, and normal tissue from mammoplasty specimens.ResultsA total of 96 samples were evaluated: 70 invasive breast carcinoma of different histologic subtypes and 26 mammoplasty normal breast tissues. All the samples were negative for IE-antigen expression. No relationship was seen between breast cancer and HCMV in this study.ConclusionsThe results of this study failed to show any relationship between HCMV and the development of breast carcinoma.

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“…On the other hand, several studies succeeded to show relationship between HCMV and breast cancer. For example, Harkins et al [ 24] in the United States demonstrated a higher expression of HCMV antigens in breast cancer compared to normal breast epithelium (97% versus 63%), and Karimi et al [ 25] also detected HCMV DNA in 26 of the 50 samples (58%) of invasive breast carcinoma by using the nested-PCR method in Iran. The differed prevalence of EBV and HCMV in various geographical areas may justify the different findings of studies Moreover, the different fixation times of tissue samples in various centers,, and time of exposure to the virus in life (early or late) may also justify some differences in the research findings.…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection of Epstein-Barr Virus and Human Cytomegalovirus Antigen Expression in Breast Cancer in Khartoum State, Sudan 2018

Aidrous

Hussein²,

Dahawi

et al. 2019

NACS

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Background: Breast cancer is a leading cause of death among women worldwide. The association between Epstein-Barr Virus (EBV) and Human Cytomegalovirus (HCMV) and breast cancer risk still remains controversial making it difficult to determine whether either, both or neither virus is causally associated with breast cancer. The aim of this study was to detect EBV and the expression of the Immediate Early Antigen (IE) of HCMV in breast cancer in Sudanese women.Method: 60 Formalin-fixed and paraffin-embedded tissue blocks from 42 ductal breast carcinoma and18 benign breast tumors (control group) were obtained from the pathology laboratories of several hospitals in Khartoum State, Sudan, and used in this study. We used PCR to detect EBV and the IE-antigen of HCMV in ductal breast carcinoma and benign breast tumor.Result: Only 3 (7%) out of 42 ductal breast carcinoma were positive for EBV DNA and none of 18 benign breast tumor (control group) were positive for EBV DNA and all 60 specimens were negative for IE-antigen of HCMV expression. Conclusion:The results of this study failed to show any relationship between EBV and HCMV and the development of breast carcinoma in the tested specimens.

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Relative Frequency of Cytomegalovirus (CMV) in Tissue Samples of Women with Breast Cancer in Sanandaj, Iran.

Cited by 3 publications

References 25 publications

Human Cytomegalovirus DNA among Women with Breast Cancer

Human Cytomegalovirus DNA among Women with Breast Cancer

Evaluation of human cytomegalovirus antigen expression in invasive breast carcinoma in a population of Iranian patients

Molecular Detection of Epstein-Barr Virus and Human Cytomegalovirus Antigen Expression in Breast Cancer in Khartoum State, Sudan 2018

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