The effects of moisture and temperature on seed longevity were studied over periods of up to 426 days in three species of Orobanche. Saturated salt solutions were used to achieve equilibrium relative humidities (e.r.h.) between 11% and 85% at temperatures of 20–60 °C. The viability equation describing loss of viability in these conditions applied to the three species such that the lower the temperature and lower the equilibrium relative humidity the greater the longevity. The initial viability differed between species with the seed lot of Orobanche crenata having the lowest viability. However, the relative effect of temperature on longevity and the relative sensitivity of seed longevity to changes in equilibrium relative humidity were the same in all species. Longevity, estimated by the standard deviation of the seed survival curves was greater in Orobanche aegyptiaca and O. crenata than in Orobanche minor, being for example about 100, 103 and 49 days, respectively, at 40 °C, 50% e.r.h. Accurate prediction of longevity is necessary to understand and quantify the population dynamics of weeds and will help the farmer to optimize control measures such as solarization. Taken in conjunction with published data on loss of viability of imbibed seeds, the annual loss of viability on a typical Eritrean farm is predicted to be about 38%.