Relative retention and column selectivity for the common polar bonded-phase columns

Normal‐Phase Chromatography

“…Figure 8.12 compares the separation of a mixture of aromatic compounds with hexane as mobile phase and (1) a cyano column, (2) a diol column, and (3) an amino column. Separation selectivity or relative retention varies moderately from column to column in Figure 8.12, as expected; proton-donor solutes are retained more strongly on amino columns relative to other solutes, and less strongly on cyano columns [26,27]. 8.12d) can be estimated (very approximately) from other published data [1].…”

“…Several studies have been reported [23][24][25][26][27] of retention for different test solutes, using each of the three types of polar-bonded-phase NPC columns (cyano, diol, amino). A more common reason for using a polar-bonded-phase column in preference to unbonded silica (primarily for assay methods) is to avoid the problems described in Section 8.5: poor separation reproducibility from run to run, slow column equilibration, or difficulty in using gradient elution for samples with a wide retention range.…”

High Performance Liquid Chromatographic Characterization of Diol Bonded Phases Synthesized via a Hydride Intermediate

“…While some differences in retention may be due to the effects of heptane instead of isooctane as the primary mobile phase constituent, the major trends observed are probably too great to be accounted for by this change alone. An illustration of the variability between diol columns is shown in Figure 4 where the two materials in this study are compared to another commercial source (DuPont) using the same solutes in the same mobile phase as in a previous report (1). In the case of AGE (white), retention is consistently longer for all compounds at all modifier concentrations than for the DuPont -diol column.…”

ΔR M as the Parameter Characterizing Chromatographic Properties of Polar Bonded Stationary Phases in Isoeluotropic Systems

“…Salotto et al [7] have compared relative retention on polar bonded stationary phases with dichloromethanen-heptane eluents for monofunctional solutes in comparison to suitable aromatic hydrocarbons as DR M vs. DR M correlations. They performed linear regressions for all monofunctional analytes on polar bonded columns, separately, for electronodonor and dipolar compounds.…”