Relaxant Action ofPlumula NelumbinisExtract on Mouse Airway Smooth Muscle

Abstract

The traditional herb Plumula Nelumbinis is widely used in the world because it has many biological activities, such as anti-inflammation, antioxidant, antihypertension, and butyrylcholinesterase inhibition. However, the action of Plumula Nelumbinis on airway smooth muscle (ASM) relaxation has not been investigated. A chloroform extract of Plumula Nelumbinis (CEPN) was prepared, which completely inhibited precontraction induced by high K+ in a concentration-dependent manner in mouse tracheal rings, but it had no effect on resting tension. CEPN also blocked voltage-dependent L-type Ca2+ channel- (VDCC-) mediated currents. In addition, ACh-induced precontraction was also completely blocked by CEPN and partially inhibited by nifedipine or pyrazole 3. Besides, CEPN partially reduced ACh-activated nonselective cation channel (NSCC) currents. Taken together, our data demonstrate that CEPN blocked VDCC and NSCC to inhibit Ca2+ influx, resulting in relaxation of precontracted ASM. This finding indicates that CEPN would be a candidate of new potent bronchodilators.

Relaxing Effect of TSU-68, an Antiangiogenic Agent, on Mouse Airway Smooth Muscle

“…According to the dose-contraction curve (see supplementary material, Fig. S1B) and the results of our previous studies [39, 44], the effective concentration 100% (EC 100 ) was 80 mM, which could maximally activate Ca 2+ permeable channels; thus, this concentration was applied. As shown in Fig.…”

“…Mouse ASM cells were isolated as described previously [39-41]. Briefly, tracheas were isolated as described above in ASM dissociation buffer (in mM: NaCl 136, KCl 5.36, KH 2 PO 4 0.44, NaHCO 3 4.16, Na 2 HPO 4 ·12H 2 O 0.34, HEPES 20, glucose 10, pH 7.1).…”

Taste Receptors Type 2 Would Not Mediate Bitter Tastant-Induced Relaxation of Airway Smooth Muscle

“…We recently further extracted compounds from bitter herbs Plumula Nelumbinis [17] and Cortex phellodendri [18] and investigated their effects on ASM contraction. The extracts, which tasted bitter, inhibited ACH-induced pre contraction of mouse ASM by inhibiting LVDCCs and NSCCs, similar to that of the known bitter tastants used in the studies described above.…”

The risk of higenamine adverse analytical findings following oral administration of plumula nelumbinis capsules

“…Plumula nelumbinis is considered to exhibit many biological effects such as anti‐inflammation, anti‐oxidation, and anti‐hypertension. A growing number of medicinal products and nutritional supplements are observed to contain plumula nelumbinis extracts and can be purchased easily . Therefore, an evaluation of the risk of an adverse analytical finding due to higenamine in doping control caused by plumula nelumbinis products is necessary.…”

“…A growing number of medicinal products and nutritional supplements are observed to contain plumula nelumbinis extracts and can be purchased easily. [5][6][7][8][9][10][11][12] Therefore, an evaluation of the risk of an adverse analytical finding due to higenamine in doping control caused by plumula nelumbinis products is necessary.…”