Relaxation Patterns of Human Gastric Corporal Smooth Muscle by Cyclic Nucleotides Producing Agents

Kim, Young Chul; Choi, Woong; Sung, Rohyun; Kim, Heon; You, Ra Young; Park, Seon Mee; Youn, Sei Jin; Kim, Mi Jung; Song, Young Jin; Xu, Wen; Lee, Sang Jin; Yun, Hyo Yung

doi:10.4196/kjpp.2009.13.6.503

Cited by 5 publications

(6 citation statements)

References 35 publications

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“…An increase in cyclic nucleotides, cGMP and cAMP, reduces the contractile tone of several types of smooth muscles, including vascular [11], gastric [12] and bladder smooth muscle [13,14]. The levels of these cyclic nucleotides are tightly controlled by their synthesis through adenylyl cyclases (ACs) and guanylyl cyclases (GCs), and their degradation through phosphodiesterases (PDEs).…”

Section: Introductionmentioning

confidence: 99%

Phosphodiesterase types 3 and 4 regulate the phasic contraction of neonatal rat bladder smooth myocytes via distinct mechanisms

Zhai

Chang

Wei

et al. 2014

Cellular Signalling

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Activation of the cyclic AMP (cAMP) pathway reduces bladder contractility. However, the role of phosphodiesterase (PDE) families in regulating this function is poorly understood. Here, we compared the contractile function of the cAMP hydrolyzing PDEs in neonatal rat bladder smooth myocytes. RT-PCR and Western blotting analysis revealed that several isoforms of PDE1-4 were expressed in neonatal rat bladder. While 8-methoxymethyl-3-isobutyl-1-methylxanthine (a PDE1 inhibitor) and BAY-60-7550 (a PDE2 inhibitor) had no effect on the carbachol-enhanced phasic contractions of bladder strips, cilostamide (Cil, a PDE3 inhibitor) and Ro-20-1724 (Ro, a PDE4 inhibitor) significantly reduced these contractions. This inhibitory effect of Ro was blunted by the PKA inhibitor H-89, while the inhibitory effect of Cil was strongly attenuated by the PKG inhibitor KT 5823. Application of Ro in single bladder smooth myocytes resulted in an increase in Ca(2+) spark frequency but a decrease both in Ca(2+) transients and in sarcoplasmic reticulum (SR) Ca(2+) content. In contrast, Cil had no effect on these events. Furthermore, Ro-induced inhibition of the phasic contractions was significantly blocked by ryanodine and iberiotoxin. Taken together, PDE3 and PDE4 are the main PDE isoforms in maintaining the phasic contractions of bladder smooth myocytes, with PDE4 being functionally more active than PDE3. However, their roles are mediated through different mechanisms.

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Section: Introductionmentioning

confidence: 99%

Phosphodiesterase types 3 and 4 regulate the phasic contraction of neonatal rat bladder smooth myocytes via distinct mechanisms

Zhai

Chang

Wei

et al. 2014

Cellular Signalling

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“…2 ). In fact, we found high concentration of sodium nitroprusside (SNP; NO-releasing compound, 5 µM) produced inhibition of spontaneous contraction (47% of the control) and basal tone (-1.1 g) in our previous study [ 12 ]. However, histamine did not inhibit spontaneous gastric contraction significantly as shown in Fig.…”

Section: Discussionmentioning

confidence: 99%

“…These findings indicate that NO-mediated pathways may be involved in histamine-induced tonic relaxation in the human stomach. Since histamine also produced tonic relaxation via activation of NO pathway, histamine might play an important role for adaptive relaxation in human stomach too [ 10 , 11 , 12 ]. As shown in the Results, histamine produced both contraction and relaxation in the same tissue, as shown in the circular smooth muscle of corpus ( Fig.…”

Section: Discussionmentioning

confidence: 99%

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H₂Receptor-Mediated Relaxation of Circular Smooth Muscle in Human Gastric Corpus: the Role of Nitric Oxide (NO)

Lee

Kim

et al. 2014

Korean J Physiol Pharmacol

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This study was designed to examine the effects of histamine on gastric motility and its specific receptor in the circular smooth muscle of the human gastric corpus. Histamine mainly produced tonic relaxation in a concentration-dependent and reversible manner, although histamine enhanced contractility in a minor portion of tissues tested. Histamine-induced tonic relaxation was nerve-insensitive because pretreatment with nerve blockers cocktail (NBC) did not inhibit relaxation. Additionally, K+ channel blockers, such as tetraethylammonium (TEA), apamin (APA), and glibenclamide (Glib), had no effect. However, NG-nitro-L-arginine methyl ester (L-NAME) and 1H-(1,2,4)oxadiazolo (4,3-A) quinoxalin-1-one (ODQ), an inhibitor of soluble guanylate cyclase (sGC), did inhibit histamine-induced tonic relaxation. In particular, histamine-induced tonic relaxation was converted to tonic contraction by pretreatment with L-NAME. Ranitidine, the H2 receptor blocker, inhibited histamine-induced tonic relaxation. These findings suggest that histamine produced relaxation in circular smooth muscle of human gastric smooth muscle through H2 receptor and NO/sGC pathways.

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“…In general, Ca 2+ is essential for contraction of visceral smooth muscles and it can be assumed that the amplitude of the contraction depends on [Ca 2+ ] i [ 17 ]. To date, voltage-dependent 'L-type' Ca 2+ channels (VDCC L ) have been reported in most excitable tissues, including human stomach [ 18 , 19 ], and VDCC L are known to play a essential role in the regulation of [Ca 2+ ] i in smooth muscles [ 20 ]. And the spontaneous contractile activity of GI muscles such as peristaltic contraction is closely related to the slow wave, and the activation of plateau Ca 2+ current on it is necessary for the excitation-contraction coupling [ 21 , 22 ].…”

Section: Discussionmentioning

confidence: 99%

Nitric Oxide-mediated Relaxation by High K⁺in Human Gastric Longitudinal Smooth Muscle

Kim

Choi

Yun

et al. 2011

Korean J Physiol Pharmacol

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This study was designed to elucidate high-K+induced response of circular and longitudinal smooth muscle from human gastric corpus using isometric contraction. Contraction from circular and longitudinal muscle stripes of gastric corpus greater curvature and lesser curvature were compared. Circular smooth muscle from corpus greater curvature showed high K+ (50 mM)-induced tonic contraction. On the contrary, however, longitudinal smooth muscle strips showed high K+ (50 mM)-induced sustained relaxation. To find out the reason for the discrepancy we tested several relaxation mechanisms. Protein kinase blockers like KT5720, PKA inhibitor, and KT5823, PKG inhibitor, did not affect high K+-induced relaxation. K+ channel blockers like tetraethylammonium (TEA), apamin (APA), glibenclamide (Glib) and barium (Ba2+) also had no effect. However, N(G)-nitro-L-arginine (L-NNA) and 1H-(1,2,4) oxadiazolo (4,3-A) quinoxalin-1-one (ODQ), an inhibitor of soluble guanylate cyclase (sGC) and 4-AP (4-aminopyridine), voltage-dependent K+ channel (KV) blocker, inhibited high K+-induced relaxation, hence reversing to tonic contraction. High K+-induced relaxation was observed in gastric corpus of human stomach, but only in the longitudinal muscles from greater curvature not lesser curvature. L-NNA, ODQ and KV channel blocker sensitive high K+-induced relaxation in longitudinal muscle of higher portion of corpus was also observed. These results suggest that longitudinal smooth muscle from greater curvature of gastric corpus produced high K+-induced relaxation which was activated by NO/sGC pathway and by KV channel dependent mechanism.

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Relaxation Patterns of Human Gastric Corporal Smooth Muscle by Cyclic Nucleotides Producing Agents

Cited by 5 publications

References 35 publications

Phosphodiesterase types 3 and 4 regulate the phasic contraction of neonatal rat bladder smooth myocytes via distinct mechanisms

Phosphodiesterase types 3 and 4 regulate the phasic contraction of neonatal rat bladder smooth myocytes via distinct mechanisms

H₂Receptor-Mediated Relaxation of Circular Smooth Muscle in Human Gastric Corpus: the Role of Nitric Oxide (NO)

Nitric Oxide-mediated Relaxation by High K⁺in Human Gastric Longitudinal Smooth Muscle

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Relaxation Patterns of Human Gastric Corporal Smooth Muscle by Cyclic Nucleotides Producing Agents

Cited by 5 publications

References 35 publications

Phosphodiesterase types 3 and 4 regulate the phasic contraction of neonatal rat bladder smooth myocytes via distinct mechanisms

Phosphodiesterase types 3 and 4 regulate the phasic contraction of neonatal rat bladder smooth myocytes via distinct mechanisms

H2Receptor-Mediated Relaxation of Circular Smooth Muscle in Human Gastric Corpus: the Role of Nitric Oxide (NO)

Nitric Oxide-mediated Relaxation by High K+in Human Gastric Longitudinal Smooth Muscle

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H₂Receptor-Mediated Relaxation of Circular Smooth Muscle in Human Gastric Corpus: the Role of Nitric Oxide (NO)

Nitric Oxide-mediated Relaxation by High K⁺in Human Gastric Longitudinal Smooth Muscle