Relaxin causes proliferation of human amniotic epithelium by stimulation of insulin-like growth factor-II

Millar, Lynnae K.; Reiny, Roxanne; Yamamoto, Sandra Y.; Okazaki, Kristie; Webster, Lisa; Bryant‐Greenwood, Gillian D.

doi:10.1067/mob.2003.80

Cited by 26 publications

(31 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In general, proteins of the IGF family have a profound impact on embryo and placenta development [5,19,26]. It was observed in a mouse model that placental weight correlated with foetal weight, and these parameters were correlated with cord blood levels of IGF-1 and -2 [5]. Moreover, IGF-1 and IGF-2 levels were also significantly lower in umbilical cord blood sera of foetuses with growth retardation compared to normal foetuses [5].…”

Section: Discussionmentioning

confidence: 80%

“…Also, IGFBP-3 has high affinity for IGF-2 [22][23][24][25] which may explain increase of its levels in culture medium during the course of the study. In general, proteins of the IGF family have a profound impact on embryo and placenta development [5,19,26]. It was observed in a mouse model that placental weight correlated with foetal weight, and these parameters were correlated with cord blood levels of IGF-1 and -2 [5].…”

Section: Discussionmentioning

confidence: 90%

“…It was observed in a mouse model that placental weight correlated with foetal weight, and these parameters were correlated with cord blood levels of IGF-1 and -2 [5]. Moreover, IGF-1 and IGF-2 levels were also significantly lower in umbilical cord blood sera of foetuses with growth retardation compared to normal foetuses [5]. In the mouse model, disruption of one of the IGF-2 alleles caused death of about 60% of mice [5].…”

Section: Discussionmentioning

confidence: 93%

“…Some factors stimulate cell differentiation and proliferation, like macrophage colony-stimulating factor (M-CSF), which was detected in cultures of human amniocytes [4]. Other factors, like IGF-2, were found on mRNA level in human amniotic epithelial cells, where they may promote proliferation [5].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Growth factors and their receptors derived from human amniotic cells in vitro

Grzywocz

Pius-Sadowska

Kłos

et al. 2014

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

Abstract:In vitro studies have shown that amnion-produced growth factors participated in angiogenesis, re-epithelialization, and immunomodulation. The aim of our study was to investigate the growth factors and receptors produced by human amnion tissue and amniotic cells. Human amnions (hAM) were isolated, and amnion circles were dissected for in vitro analysis. Some amnion fragments were digested by the use of different methods to obtain two cell fractions, which were analysed for mesenchymal and epithelial cell markers. Amniotic circles and human amniotic cell fractions were cultured in a protein-free medium. Proteins secreted into the culture medium were analysed with a human growth factor antibody array. Conditioned culture media were added to human umbilical vein epithelial cells (HUVECs) to test for stimulation of migration (scratch test) and proliferation (Ki67 expression). Fraction 1 cells expressed both cytokeratin and mesenchymal cell markers which indicated that it was composed of a mixture of human amnion epithelial cells (hAECs) and mesenchymal stromal cells (hAMSCs). Fraction 2 cells mainly expressed cytokeratin and, therefore, were designed as hAECs. Secretion of proteins by the cultured cells increased with time. The hAM cultures secreted EGF-R, IGF, and IGFBP-2, -3 and -6; Cell Fraction 1 secreted NT-4, whereas Cell Fraction 2 secreted G-CSF, M-CSF, and PDGF. Conditioned media of hAM cultures stimulated HUVECs migration. We have showed for the first time that human amnions and amniotic cells secreted IGFBP-6, MCSF-R, PDGF-AB, FGF-6, IGFBP-4, NT-4, and VEGF-R3. We found that Cell Fraction 1, Cell Fraction 2, and the whole amnion secreted different proteins, possibly due to different proportions of amnion-derived cells and different cell-cell interactions. The hAM cell factors remained functional in vitro and induced intensified migration of HUVECs. The growth factors and receptors found in amnion or amniotic cell media might be used for regenerative medicine.

show abstract

Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Growth factors and their receptors derived from human amniotic cells in vitro

Grzywocz

Pius-Sadowska

Kłos

et al. 2014

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

show abstract

“…The tissue surrounding a stab wound in FM has been shown to be viable for up to 12 days but the wound remained unhealed (Devlieger et al, 2000a). Millar et al (2003) demonstrated increased growth of amnion epithelium with IGF-II. Using a rabbit model, Devlieger et al (2000bDevlieger et al ( & 2003 have shown some success in incorporating plugs into iatrogenic ruptured sites.…”

Section: Investigations Targeting the Potential Prevention Of Fm Weakmentioning

confidence: 99%