Release and retention of calcium and phosphorus from various dairy products in the oral fluid

Patocka, G.; Ja, Hargreaves

doi:10.1016/0958-6946(91)90002-p

Cited by 3 publications

(2 citation statements)

References 5 publications

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“…It has been suggested that the CPP may prevent the precipitation of insoluble calcium phosphate in the distal intestine, and thereby increase the amount of soluble intralumenal calcium available for paracellular absorption between mucosal cells [4]. Furthermore, CPP have been shown to inhibit caries lesions by recalcification of the dental enamel [5]. Thus, CPP can be considered as 'physiologically functional ingredients' of dairy products that are available as natural components to prevent teeth and bone demineralization.…”

Section: Introductionmentioning

confidence: 99%

Semi‐preparative isolation of phosphopeptides derived from bovine casein and dephosphorylation of casein phosphopeptides

Goepfert¹,

Meisel²

1996

Nahrung

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Semi-preparative isolation of casein phosphopeptides (CPP) from a tryptic hydrolysate of bovine casein was performed applying a three-step procedure consisting of solid phase extraction, reversed phase HPLC and ion exchange chromatography. Dephosphorylation of CPP was achieved using immobilized alkaline phosphatase. The purified phosphopeptides and their dephosphorylated forms obtained by these methods are suitable for comparative studies on biological activities, especially mineral binding and immunmodulation.

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Section: Introductionmentioning

confidence: 99%

Semi‐preparative isolation of phosphopeptides derived from bovine casein and dephosphorylation of casein phosphopeptides

Goepfert¹,

Meisel²

1996

Nahrung

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“…These phosphopeptides have been shown to have chelating properties, corresponding to their possible interaction of the negatively charged phosphoseryl residues with nutritional inorganic cations, especially calcium, iron and zinc [4]. There is evidence, that CPPs inhibit formation of caries lesions by recalcification of the dental enamel [5,6]. Moreover, CPPs are suggested to enhance calcium availability in intestinal absorption by solubilisation, thus inhibiting the characteristic loss of calcium as a result of crystallisation or precipitation of insoluble calcium phosphate in the small intestine [7,8].…”

Section: Introductionmentioning

confidence: 99%

Cytochemical assessment of phosphopeptides derived from casein as potential ingredients for functional food

Hartmann¹,

Meisel

2002

Nahrung

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Phosphopeptides derived from casein hydrolysis are suggested to have beneficial effects beyond their mere nutritive value by enhancing availability of dietary minerals, especially calcium, iron and zinc. Apart from a possible positive action the potential may exist for adverse effects that could impose restrictions to their widespread application in functional foods for human nutrition. In the present work, various case-inophosphopeptide (CPP) preparations were assessed using different human cell culture model systems in order to estimate their cytotoxic potential and their influence on epithelial properties and differentiation of human intestinal cells (Caco-2). The general cytotoxic potential of CPPs was tested using the AlphaTox NR assay. Structural and functional differentiation of intestinal Caco-2 cells was determined by measuring transepithelial electrical resistance and activity of brush border associated alkaline phosphatase over an extended cultivation period. No loss in cell viability with respect to membrane integrity could be detected, as uptake and retention of neutral red dye (AlphaTox assay) into HeLa cells was not affected by the presence of CPPs. Cytochemical assays conducted on epithelial cells (Caco-2) showed no disturbance of normal cell development and differentiation with respect to structural as well as functional differentiation markers. As all CPPs tested did not provoke any adverse effects in terms of cytotoxicity and showed no impairment of cell differentiation and monolayer integrity of human intestinal cells, it may be supposed that CPPs do not provoke a cytotoxic response in vivo in the cells assayed in the present study.

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Short-term consumption of probiotic-containing cheese and its effect on dental caries risk factors

Ahola

Yli-Knuuttila

Suomalainen

et al. 2002

Archives of Oral Biology

309

192

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Release and retention of calcium and phosphorus from various dairy products in the oral fluid

Cited by 3 publications

References 5 publications

Semi‐preparative isolation of phosphopeptides derived from bovine casein and dephosphorylation of casein phosphopeptides

Semi‐preparative isolation of phosphopeptides derived from bovine casein and dephosphorylation of casein phosphopeptides

Cytochemical assessment of phosphopeptides derived from casein as potential ingredients for functional food

Short-term consumption of probiotic-containing cheese and its effect on dental caries risk factors

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