Release of 5‐Hydroxytryptamine and Histamine from Rat Mast Cells¹

Abstract

The release of 5‐hydroxytryptamine (5‐HT) and histamine from rat peritoneal mast cells was studied. Major variations of amine content per cell were noted. Compound 48/80, n‐decylamine, polymyxin B, phospholipase A and distilled water released 5‐HT and histamine. The concentration‐response curve for 5‐HT to 48/80 was parallel to that of histamine. The number of histamine molecules released, considered as a ratio of the number of 48/80 molecules in the medium, ranged from 4 to over 40, whereas the corresponding ratio for 5‐HT ranged from less than 1 to approximately 2. The amine release by 48/80 was dependent upon pH and temperature and took place within 20 sec at 22° C. Certain enzyme inhibitors such as allicin (SH group inhibitor) and ninhydrin (NH2 group inhibitor) blocked the amine release. Dinitrophenol inhibited the release only in glucose‐free medium. Sodium cyanide, 10‐4M, and anoxia inhibited the release of neither amine. The results suggest that 5‐HT is released from mast cells by the same mechanism as is histamine. The data are in agreement with previous evidence from this laboratory favouring an enzymatic mechanism of release of histamine by polymer amine releasing agents.

Antipruritic Effect of Cnidii Monnieri Fructus (Fruits of Cnidium monnieri CUSSON).

“…from mast cell. [13][14][15][16] Although histamine is the major chemical mediator inducing itching, histamine did not induce scratching behavior in ddY mice as described in the previous paper. 1) CM-ext significantly inhibited compound 48/80-induced scratching behaviors of ddY mice without influence on spontaneous locomotion.…”

The effect of lipopolysaccharide, interleukin‐1 and tumour necrosis factor on the hepatic accumulation of 5‐hydroxytryptamine and platelets in the mouse

“…Mast cells in rodents contain and release 5-HT in addition to histamine (Moran et al, 1962;Prouvost-Danon et al, 1966). Thus, we used mice known to be deficient in mast cells (Kitamura et al, 1978;Suda et al, 1985) to ascertain the role played by these cells in the mobilization of 5-HT into the liver.…”

Characteristics of Histamine Release from Rat Mast Cells Induced by a Bracken Toxin, Braxin A1

“…These facts suggest that braxin Al, as well as X537A or chlorpromazine (33), has a cytotoxic effect on plasma and perigranular membranes to increase permeability and to release histamine. In addition, the action of braxin Al could be distinguished from the simple lytic effect of decylamine, because braxin Al did not release histamine at 0°C; decylamine causes hista mine release at low temperatures (30,34). Some similarities were found in the hista mine-releasing action of braxin Al and X537A, as mentioned above, but the actions were quite different in their dependency on extracellular inorganic ions.…”