Release of Covalently Bound Metabolites of Organophosphate Pesticides from Synthetic Dialkylphosphoseryl Peptides by Supercritical Fluid Extraction

Paquet, Alenka; Khan, Shahamat U.

doi:10.1021/jf00051a054

Cited by 11 publications

(10 citation statements)

References 7 publications

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“…Very early, 31 P NMR has been proposed as an analytical tool to measure OP levels at the 0.5 ppm level in cole crops such as broccoli and cabbage, where traditional gas chromatographic techniques are prone to errors due to interference with non-phosphorus coextractants [195]. However, most applications of 31 P NMR in the field of OP deal with reactivity issues [196], the characterization of OP degradation products [197,198] and metabolites [199], or toxic contaminant analysis of OP formulations [200]. The use of 31 P NMR for OP residue analysis in soil, water and other environmental samples has been reviewed [201].…”

Section: Organophosphorus Pesticidesmentioning

confidence: 99%

NMR Spectroscopy in Food Analysis

Spyros

Dais

2012

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During the last two decades, the use of NMR spectroscopy for the characterization and analysis of food materials has flourished, and this trend continues to increase today. Currently, there exists no book that fulfils specifically the needs of food scientists that are interested in adding or expanding the use of NMR spectroscopy in their arsenal of food analysis techniques. Current books and monographs are rather addressed to experienced researchers in food analysis providing new information in the field. This book, written by acknowledged experts in the field, fills the gap by offering a day to day NMR guide for the food scientist, affording not only the basic theoretical aspects of NMR spectroscopy, but also practical information on sample preparation, experimental conditions and data analysis. Current developments in the field covered in this book are the availability of solid state NMR experiments such as CP/MAS and more importantly HR-MAS NMR for the analysis of semisolid foods, and the increasing use of chemometrics to analyze NMR data in food metabonomics. Moreover, this book contains an up to date discussion of MRI in food analysis including topics such as food processing and natural changes in food such as ripening. The book is a compact and complete source of information for food scientists who wish to apply methodologies based on NMR spectroscopy in food analysis. It contains information so far scattered in the primary literature, in NMR treatises and food analysis books, in a concise format that makes it appealing to food scientists who have no or minimal experience in magnetic resonance techniques. The inclusion of practical information about NMR instrumentation, experiment setup, acquisition and spectral analysis for the study of different food categories make this book a hands-on manual for food scientists wishing to implement novel NMR spectroscopy-based analytical techniques in their field.

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Section: Organophosphorus Pesticidesmentioning

confidence: 99%

NMR Spectroscopy in Food Analysis

Spyros

Dais

2012

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“…Also, organophosphosphates and the metabolites produced are expected to bind to plant proteins covalently (Paquet and Khan 1995), thereby forming inextricable bound residues, which further may be stored in vacuoles and cell walls or metabolized.…”

Section: Phorate Degradation and Metabolite Formationmentioning

confidence: 99%

“…Organophosphates are known to phosphorylate the serine residues in the active centers of esterases (Paquet and Khan 1995), thus destroying the enzyme activity irreversibly and detoxifying the pesticides. Thus, esterases may be involved in phorate metabolism in B. juncea, and increased activities should be useful as defense of the effect of phorate exposure.…”

Section: Measurement Of Hydrolytic and Antioxidative Enzymesmentioning

confidence: 99%

Phytotransformation of Phorate by Brassica juncea (Indian Mustard)

Rani

Padole

Juwarkar

et al. 2011

Water Air Soil Pollut

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Over 5 days, Brassica juncea removed 54% of the highly toxic insecticide phorate from the medium with the formation of phorate sulfoxide in small quantity. The loss of phorate from the medium followed first-order kinetics. The half-life of phorate disappearance from water decreased by~4.5-fold in the presence of B. juncea. Mild phorate phytotoxicity was evident from the elevated activities of the antioxidative enzymes like glutathione-disulfide reductase, glutathione S-transferase, superoxide dismutase, and catalase in the plants. Nevertheless, the ubiquitous antioxidative peroxidase was not significantly increased, nor the total glutathione content, due to phorate exposure. Phosphotriester bond hydrolysis and glutathione S-transferase-mediated conjugation seemed to be the key reactions for phorate metabolism by B. juncea. From the limited information available, for the first time, a tentative mapping of phytotransformation pathways was performed.

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“…7). Paquet and Khan [107] investigated the efficiency of supercritical fluid extraction (SFE) in plant component analysis in order to find a way to extract metabolites of OP pesticides covalently bound to serine residues of proteins in plants or grains. The phosphorylation of proteins with pesticides can reduce the nutritive value of cereal grain proteins [108].…”

Section: Pesticide Residue Analysis In Environment and Foodmentioning

confidence: 99%

Use of NMR techniques for toxic organophosphorus compound profiling

Koskela

2010

Journal of Chromatography B

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Release of Covalently Bound Metabolites of Organophosphate Pesticides from Synthetic Dialkylphosphoseryl Peptides by Supercritical Fluid Extraction

Cited by 11 publications

References 7 publications

NMR Spectroscopy in Food Analysis

NMR Spectroscopy in Food Analysis

Phytotransformation of Phorate by Brassica juncea (Indian Mustard)

Use of NMR techniques for toxic organophosphorus compound profiling

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