Release of Cu2+ from a copper-filled TiO2 coating in a rabbit model for total knee arthroplasty

Mauerer, Andreas; Lange, Bastian; Welsch, Goetz H.; Heidenau, Frank; Adler, Werner; Forst, Raimund; Richter, R.H.

doi:10.1007/s10856-013-5116-x

Cited by 15 publications

(19 citation statements)

References 33 publications

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“…These coatings (4xCu-TiO 2 ) caused a decrease in the number of adhering bacteria (more than 6 log) and a reduction in bacterial growth in the surrounding medium, suggesting a release of Cu ions (180). The same authors confirmed these results in further studies both in vitro (181) and in animal models (182). Hang et al (183) fabricated Cu-TiO 2 nanotube coatings with different Cu contents.…”

Section: •-mentioning

confidence: 81%

Antifouling and antimicrobial biomaterials: an overview

Francolini

Vuotto

Piozzi

et al. 2017

APMIS

263

220

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The use of implantable medical devices is a common and indispensable part of medical care for both diagnostic and therapeutic purposes. However, as side effect, the implant of medical devices quite often leads to the occurrence of difficult‐to‐treat infections, as a consequence of the colonization of their abiotic surfaces by biofilm‐growing microorganisms increasingly resistant to antimicrobial therapies. A promising strategy to combat device‐related infections is based on anti‐infective biomaterials that either repel microbes, so they cannot attach to the device surfaces, or kill them in the surrounding areas. In general, such biomaterials are characterized by antifouling coatings, exhibiting low adhesion or even repellent properties towards microorganisms, or antimicrobial coatings, able to kill microbes approaching the surface. In this light, the present overview will address the development in the last two decades of antifouling and antimicrobial biomaterials designed to potentially limit the initial stages of microbial adhesion, as well as the microbial growth and biofilm formation on medical device surfaces.

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Section: •-mentioning

confidence: 81%

Antifouling and antimicrobial biomaterials: an overview

Francolini

Vuotto

Piozzi

et al. 2017

APMIS

263

220

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“…Such behavior could represent a good model for bacteremia without ongoing sepsis. Several antibacterial coatings (antibiotics, antiseptics, metal ions, non‐metal ions, or organic molecules) of implants in orthopedics have been developed, and the ability of such implants to minimize bacterial adhesion or inhibit biofilm formation has been investigated. However, we believe that to date, the antibacterial activity against hematogenous infection has not been investigated.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Activity of Ag‐Hydroxyapatite Coating Against Hematogenous Infection by Methicillin‐ResistantStaphylococcus aureusin the Rat Femur

Kobatake

Miyamoto

Hashimoto

et al. 2019

Journal Orthopaedic Research

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Several antibacterial materials have been developed to prevent periprosthetic joint infection and thus prevent serious complications for patients and surgeons. However, no study has addressed the activity of antibacterial materials against hematogenous infection. The present study evaluated the antibacterial activity of a silver-containing hydroxyapatite-coated implant against methicillin-resistant Staphylococcus aureus (MRSA) hematogenous infection. Implants coated with hydroxyapatite and silver-hydroxyapatite were inserted into rats' right and left femurs, respectively, after which the animals were infected with S. aureus via a tail vessel. About 10 7 colony-forming units was the optimal bacterial number for the establishment of S. aureus hematogenous infection. Bacterial loads and C-reactive protein in the blood were measured to confirm bacteremia and inflammation. Fourteen days after the infection, bacterial loads were statistically lower in the femurs containing silver-hydroxyapatite-coated implants than in those with hydroxyapatite-coated implants (p = 0.022). Thus, silver-hydroxyapatite-coated implants might provide antibacterial activity against MRSA hematogenous infection in the postoperative period.

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“…Initiatives to minimize bacterial colonization at the time of insertion have been studied in the past with slow permeation in the clinical setting [1,39,48]. Several preclinical and clinical studies support the use of copper alloy touch surfaces to reduce the burden of bacteria in healthcare settings [10,11,33], but few studies support the use of copper implants to reduce periprosthetic infections [6,18,27,41]. The ideal antibacterial coating should be biocompatible, thin, dense, and firm with precise decay of the active material.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial and Biocompatible Titanium-Copper Oxide Coating May Be a Potential Strategy to Reduce Periprosthetic Infection: An In Vitro Study

Norambuena

Patel

Karau

et al. 2017

Clinical Orthopaedics &Amp; Related Research

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Background Periprosthetic infections are devastating for patients and more efficacious preventive strategies are needed. Surface-modified implants using antibacterial coatings represent an option to cope with this problem; however, manufacturing limitations and cytotoxicity have curbed clinical translation. Among metals with antibacterial properties, copper has shown superior in vitro antibacterial performance while maintaining an acceptable cytotoxicity profile. A thin film containing copper could prevent early biofilm formation to limit periprosthetic infections. This pilot study presents the in vitro antibacterial effect, cytotoxicity, and copper ion elution pattern of a thin film of titanium-copper oxide (TiCuO). Questions/purposes (1) Do titanium alloy (Ti6Al4V) discs coated with a thin film of TiCuO reduce Staphylococcus epidermidis biofilm and planktonic cell density compared with uncoated discs? (2) Do Ti6Al4V discs coated with a thin film of TiCuO affect normal human osteoblast viability compared with untreated cells? (3) Is copper ion concentration generated by coated discs lower than previously published copper ion concentrations that cause 50% toxicity in similar human cell lines in vitro (TC50)? Methods Ninety Ti6Al4V discs (12.5 mm diameter; 1.25 mm thick) were used in this study. Seventy-two Ti6Al4V discs were coated with a thin film of either titanium oxide (TiO) or TiCuO containing 20%, 40%, or 80% copper using high-power impulse magnetron sputtering (HiPIMS). Eighteen Ti6Al4V discs remained uncoated for control purposes. We tested antibacterial properties of S epidermidis grown on The institution of one or more of the authors (GAN, RP, MK, CCW, PJJ, KEB, ADH, RJS) has received funding in excess of USD 100,000 from Codelco (Codelco Lab, Santiago, Chile; FP00076314-01-S01) and in excess of USD 10,000 from the Mayo Clinic. With the exception of one of the authors (RJS), the investigators have not personally received any money related to this study. One of the authors certifies that he (RJS), or a member of his immediate family, has received or may receive payments or benefits, during the study period, in an amount of USD 10,000 to USD 100,000 from Biomet Inc (Warsaw, IN, USA Clinical Orthopaedics and Related Research ®A Publication of The Association of Bone and Joint Surgeons® discs in wells containing growth medium. After 24 hours, planktonic bacteria as well as biofilms removed by sonication were quantitatively cultured. Annexin/Pi staining was used to quantify in vitro normal human osteoblast cell viability at 24 hours and Day 7, respectively. Copper elution was measured at Days 1, 2, 3, 7, 14, and 28 using an inductively coupled plasma mass spectrometer to analyze aliquots of culture medium. Copper ion concentration achieved at 24 hours was compared with previously published TC50 for gingival fibroblast, a phenotypically similar cell line with available data regarding copper ion exposure. Results Discs coated with TiCuO 80% copper showed greater biofilm and planktonic cell density re...

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Release of Cu2+ from a copper-filled TiO2 coating in a rabbit model for total knee arthroplasty

Cited by 15 publications

References 33 publications

Antifouling and antimicrobial biomaterials: an overview

Antifouling and antimicrobial biomaterials: an overview

Antibacterial Activity of Ag‐Hydroxyapatite Coating Against Hematogenous Infection by Methicillin‐ResistantStaphylococcus aureusin the Rat Femur

Antibacterial and Biocompatible Titanium-Copper Oxide Coating May Be a Potential Strategy to Reduce Periprosthetic Infection: An In Vitro Study

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