Release of endothelin-1 from human endocardium after radiofrequency catheter ablation and coronary angioplasty: comparative results

Davlouros, Periklis; Simeonidou, Eftihia; Tsakas, Sotiris; Vlachojannis, Ioannis; Alexopoulos, Dimitrios; Manolis, Antonis S.

doi:10.1016/j.ijcard.2004.04.010

Cited by 7 publications

(5 citation statements)

References 42 publications

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“…The impact on endothelial damage caused by AF catheter ablation is well known [2], [4]. Bulava et al [8] showed changes after catheter ablation on pro-thrombotic and endothelial damage markers, and we confirm their finding showing significantly increased vWF directly after procedure.…”

Section: Discussionsupporting

confidence: 83%

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Effects of Radiofrequency Catheter Ablation of Atrial Fibrillation on Soluble P-Selectin, Von Willebrand Factor and IL-6 in the Peripheral and Cardiac Circulation

et al. 2014

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BackgroundCatheter ablation (CA) of atrial fibrillation (AF) is associated with inflammatory response, endothelial damage and with increased risk of thrombosis. However, whether these processes differ in peripheral and cardiac circulation is unknown.MethodsPlasma markers (von Willebrand factor (vWf), soluble P-selectin (sPsel) and interleukin-6 (IL-6)) were measured by ELISA at three time points in 80 patients (62±10 years, 63% males, 41% paroxysmal AF) undergoing CA. These were at baseline – from femoral vein (FV) and left atrium (LA) before ablation; directly after ablation – from the pulmonary vein (PV), LA and FV; and 24 hours after procedure – from a cubital vein (CV).ResultsThe levels of vWF and IL6 – but not sP-sel – increased significantly 24h after procedure (p<0.001). Baseline vWF was significantly associated with persistent AF (Beta = .303, p = 0.006 and Beta = .300, p = 0.006 for peripheral and cardiac levels, respectively), while persistent AF (Beta = .250, p = 0.031) and LAA flow pattern (Beta = .386, p<0.001) remained associated with vWF in cardiac blood after ablation. Advanced age was significantly associated with IL6 levels at baseline and after ablation in peripheral and cardiac blood. There were no clinical, procedural or anti-coagulation characteristics associated with sP-sel levels in cardiac blood, while peripheral sP-sel levels were associated with hypertension before (Beta = −.307, p = 0.007) and with persistent AF after ablation (Beta = −.262, p = 0.020).ConclusionsvWF levels are higher in persistent AF and are associated with LAA rheological pattern after AF ablation. Increase of peripheral vWF and IL6 levels after procedure supports current AF ablation management with careful control of post-procedural anticoagulation to avoid ablation-related thromboembolism.

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Section: Discussionsupporting

confidence: 83%

“…An association between rhythm outcomes after catheter ablation and markers of inflammation is known [2], [3], [4]. Recently, we demonstrated that AF ablation leads to changes in inflammatory biomarkers that had an impact on AF recurrences during 6 months follow-up after procedure [3], [5].…”

Section: Introductionmentioning

confidence: 96%

Effects of Radiofrequency Catheter Ablation of Atrial Fibrillation on Soluble P-Selectin, Von Willebrand Factor and IL-6 in the Peripheral and Cardiac Circulation

et al. 2014

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“…An increase in endothelin-1 concentrations in coronary sinus and systemic circulation following coronary angioplasty has been demonstrated in numerous studies. [24][25][26] Potential mechanisms leading to increased levels of endothelin-1 in this situation include mechanical stretch, hypoxia, and elevated angiotensin II concentrations, all involving increased oxidative stress. 24 Accordingly, in our control patients, systemic concentrations of endothelin-1 increased following angioplasty.…”

Section: Discussionmentioning

confidence: 99%

Endothelin-1 in Humans is Increased by Oxygen-Derived Radicals Ex Vivo and in Vivo

Knappe¹,

Sill²,

Tharun³

et al. 2007

Journal of Investigative Medicine

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Endothelin-1, angiotensin II, and oxygen-derived radicals are pivotal factors in the development and progression of atherosclerosis. In vitro studies suggest that generation of oxygen-derived radicals by angiotensin II is an important mechanism increasing endothelin-1 synthesis, which consecutively may trigger effects such as cell proliferation and hypertrophy. The aim of this study was to confirm our previous data in an ex vivo and an in vivo setting. Explanted segments of internal mammary arteries were analyzed for big endothelin-1 expression following incubation with xanthine oxidase, angiotensin II, superoxide dismutase, and catalase to stimulate or to specifically inactivate oxygen-derived radicals. Endothelin-1 concentrations were determined by immunostaining and enzyme-linked immunosorbent assay. Further, oxypurinol was given to patients undergoing coronary angioplasty, a procedure known to increase plasma endothelin-1 concentrations. Angiotensin II and xanthine oxidase dose-dependently increased big endothelin-1 concentrations (p < .01 and p < .0001); the effects could be inhibited by coincubation with superoxide dismutase and catalase as determined by both semiquantitative immunofluorescence and enzyme-linked immunosorbent assay (p < .01). Patients undergoing coronary angioplasty exhibited significantly elevated big endothelin-1 concentrations 60 minutes after angioplasty (p = .03); in patients also receiving oxypurinol immediately after angioplasty, big endothelin-1 concentrations decreased (p = .001). Our results may explain the association between elevated angiotensin II levels, increased oxidative stress, and increased endothelin-1 concentrations in atherosclerosis. The data therefore support the concept that oxygen-derived free radicals stimulate the release of endothelin-1, which subsequently induces effects such as proliferation and enhanced agonist-induced vasoconstriction, previously attributed directly to angiotensin II.

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“…The lack of thromboembolic events in these patients indicates that if patients with a high risk of symptomatic events are monitored closely, the application of oral anti-coagulants may be safely discontinued. In fact, it is widely known that a correlation is present between the outcome of catheter ablation and the expression of inflammation markers in the circulation [26][27][28]. Recently, it was shown that a successful procedure of AF ablation can change the profiles of inflammatory markers and affect the chance of AF recurrence following the procedure of AF ablation [27,29].…”

Section: Discussionmentioning

confidence: 99%

Radiofrequency ablation reduces expression of SELF by upregulating the expression of microRNA-26a/b in the treatment of atrial fibrillation

Dai

Jiang

Luo

et al. 2022

J Interv Card Electrophysiol

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Background In this study, we aimed to investigate the role of miR-26a and miR-26b in the management of AF. Methods Real-time PCR was carried out to determine plasma microRNA expression in AF patients pre- and post-radiofrequency ablation. The correlation between the expression of SELP and miR-26a/miR-26b was also studied using luciferase assays to establish a miR-26a/miR-26b/SELP signaling pathway. Results The relative expression of SELP reached its peak in pre-ablation AF ( +) patients, while ablation treatment reduced the expression of SELP in AF ( +) patients. Similarly, AF pigs showed dysregulation of miR-26a/b and SELP, thus verifying the involvement of miR-26a/b and SELP in AF. Meanwhile, the regulatory association between SELP and miR-26a/b was also investigated, and the results showed that the presence of pre-miR-26a/b increased the levels of miR-26a/b and inhibited the mRNA/protein expression of SELP. Finally, using bioinformatic tools and luciferase assays, SELP mRNA was confirmed as the target of miR-26a/b, which affected the effect of AF ablation treatment. Conclusions RFA helped to restore circulating levels of miR-26, which were reduced in atrial fibrillation. Meanwhile, miR-26 is a potential cause for the elevated plasma levels of pro-thrombogenic SELP in that disease.

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Release of endothelin-1 from human endocardium after radiofrequency catheter ablation and coronary angioplasty: comparative results

Cited by 7 publications

References 42 publications

Effects of Radiofrequency Catheter Ablation of Atrial Fibrillation on Soluble P-Selectin, Von Willebrand Factor and IL-6 in the Peripheral and Cardiac Circulation

Effects of Radiofrequency Catheter Ablation of Atrial Fibrillation on Soluble P-Selectin, Von Willebrand Factor and IL-6 in the Peripheral and Cardiac Circulation

Endothelin-1 in Humans is Increased by Oxygen-Derived Radicals Ex Vivo and in Vivo

Radiofrequency ablation reduces expression of SELF by upregulating the expression of microRNA-26a/b in the treatment of atrial fibrillation

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