Release of Fluconazole from Contact Lenses Using a Novel In Vitro Eye Model

Phan, Chau-Minh; Bajgrowicz, Magdalena; Gao, Huayi; Subbaraman, Lakshman N.; Jones, Lyndon

doi:10.1097/opx.0000000000000760

Cited by 23 publications

(23 citation statements)

References 49 publications

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“…The results for HPMC and PEG in this study are in agreement with previous claims for DACP [11], in which HPMC is released from the lens surface mainly during lens insertion, and PEG is released within the first few hours to provide moisture in the early hours of wear [11]. The OcuFlow device [29] appears to be an ideal platform for investigating the release of both drugs [22,23,28] and wetting agents/polymers from contact lens materials and should help to provide valuable data on the concept of developing future materials that deliver such agents to the ocular surface.…”

Section: Discussionsupporting

confidence: 90%

“…Based on the LCMS studies, the MW of PEG in the DACP blister pack is between 200 and 600 Da, which is low enough to be incorporated in high amounts within the lens material, following its placement into the blister pack [11]. However, the small molecular size also results in a faster release of the agent from the CL and this typical observation of burst release kinetics has been extensively reported for release of drugs from CLs [22,23,28].…”

Section: Discussionmentioning

confidence: 99%

“…Under these circumstances, a flow system, such as the OcuFlow system utilised in this study, may be a better model to predict actual conditions found in the eye during normal wear. The release of components from CLs is much slower under a flow system, and is affected by tear volume and flow rate [22,28,32,38]. However, for very hydrophilic compounds such as PEG and HPMC, the release may still be rapid.…”

Section: Discussionmentioning

confidence: 99%

“…However, this model does not simulate appropriate tear volume and natural tear flow [20,21], air exposure, and mechanical rubbing, which are factors which can significantly impact release kinetics. We have recently developed an in vitro model (OcuFlow) that allows lenses to be examined on a corneal-shaped profile that exposes the lens to a blinkactuator that mimics the action of the lid blinking, mimics the inter-blink period, and also flows an artificial tear fluid over the lens at physiologically relevant rates of replenishment and quantity, and permits any elute from the lens to be captured and subsequently analysed [22,[28][29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

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Determination of the release of PEG and HPMC from nelfilcon A daily disposable contact lenses using a novel in vitro eye model

Phan¹,

Walther²,

Smith

et al. 2018

Journal of Biomaterials Science, Polymer Edition

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The traditional method to measure release of components from CLs is a vial containing a static volume of PBS (phosphate buffered saline). However, this model does not simulate physiologically relevant tear volume and natural tear flow, air exposure, and mechanical rubbing. These factors can significantly impact release kinetics. We have developed an in vitro eye model (OcuFlow) that simulates these parameters. The aim of the study was to measure the release of PEG (polyethylene glycol), and HPMC (hydroxypropyl methylcellulose) from a daily disposable hydrogel contact lens material (nelfilcon A; Dailies AquaComfort PLUS; DACP;) over 24 hrs using the OcuFlow platform. The elution of PEG and HPMC from DACP lenses was analyzed using LCMS (liquid chromatography mass spectrometry). The release of all wetting agents from the lenses followed a burst release pattern, which occurred within the first 1.5 hrs (P < 0.05). The release of PEG was greater than that of HPMC (P < 0.05). The amount of PEG and HPMC released at any given time was less than 1% of the amount in the blister pack solution. Our results suggest that HPMC and PEG are rapidly released from the CL.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Determination of the release of PEG and HPMC from nelfilcon A daily disposable contact lenses using a novel in vitro eye model

Phan¹,

Walther²,

Smith

et al. 2018

Journal of Biomaterials Science, Polymer Edition

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“…22,27–29 The setup of the eye model with attachment to a microfluidic system is shown in Figure 1. The humidity was maintained using a humidifier and hygrometer to a minimum of 50% air moisture.…”

Section: Methodsmentioning

confidence: 99%

Release of Moxifloxacin from Contact Lenses Using an In Vitro Eye Model: Impact of Artificial Tear Fluid Composition and Mechanical Rubbing

Phan

Bajgrowicz-Cieslak

Subbaraman

et al. 2016

Trans. Vis. Sci. Tech.

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PurposeThe aim of this study was to evaluate and compare the release of moxifloxacin from a variety of daily disposable (DD) contact lenses (CLs) under various conditions using a novel in vitro eye model.MethodsFour commercially available DD conventional hydrogel (CH) CLs (nelfilcon A, omafilcon A, etafilcon A, and ocufilcon B) and three silicone hydrogel (SH) CLs (somofilcon A, narafilcon A, and delefilcon A) were evaluated. These lenses were incubated in moxifloxacin for 24 hours. The release of the drug was measured using a novel in vitro model in three experimental conditions: (1) phosphate buffered saline (PBS); (2) artificial tear solution (ATS) containing a variety of proteins and lipids; and (3) ATS with mechanical rubbing produced by the device.ResultsOverall, CH CLs had a higher drug release than SH CLs (P < 0.05) under all conditions. Typically, a higher drug release was observed in PBS than ATS (P < 0.05). For CH, drug release was found to be higher in ATS with rubbing than PBS or ATS (P < 0.05). For most lens types, ATS with rubbing produced higher drug release than ATS alone (P < 0.05). Generally, the release kinetics for all conditions were sustained over the 24-hour testing period, and no burst release was observed (P < 0.05).ConclusionsMoxifloxacin release from a CL into ATS is lower when compared to release into PBS. When mechanical rubbing is introduced, the amount of drugs released is increased.Translational RelevanceResults suggest that sophisticated in vitro models are necessary to adequately model on-eye drug release from CL materials.

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Analysis of polyvinyl alcohol release from commercially available daily disposable contact lenses using an in vitro eye model

et al. 2018

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The purpose of this work was to determine the release of polyvinyl alcohol (PVA) from etafilcon A, omafilcon A, and nelfilcon A daily disposable hydrogel contact lenses using a novel in vitro model. PVA is an ocular lubricant that can be found in multiple formulations of artificial tears. Nelfilcon A innately contains PVA, so only the release of PVA from this lens was evaluated. Etafilcon A and omafilcon A lenses were incubated in a PBS solution containing PVA. The release of PVA was evaluated using a novel in vitro blink platform with Milli‐Q water and PBS under various blink conditions and flow rates. Nelfilcon A lenses significantly released more PVA than other lenses at 0.5 and 1.5 h in both PBS and Milli‐Q water ( p < 0.001). For nelfilcon A, there was no statistical significance between the release profiles of PVA between the blink and no‐blink conditions, or for the various flow rates ( p > 0.05). All tested groups and lenses showed a burst release within the first 4.5 h and rapidly plateaued thereafter. The current study demonstrates that releasable PVA (whether through uptake or through being inherently available from the material) is loosely bound on hydrogel lenses, and the majority is released within 4.5 h. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1662–1668, 2019.

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Release of Fluconazole from Contact Lenses Using a Novel In Vitro Eye Model

Cited by 23 publications

References 49 publications

Determination of the release of PEG and HPMC from nelfilcon A daily disposable contact lenses using a novel in vitro eye model

Determination of the release of PEG and HPMC from nelfilcon A daily disposable contact lenses using a novel in vitro eye model

Release of Moxifloxacin from Contact Lenses Using an In Vitro Eye Model: Impact of Artificial Tear Fluid Composition and Mechanical Rubbing

Analysis of polyvinyl alcohol release from commercially available daily disposable contact lenses using an in vitro eye model

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