Release of leukotriene B4 from human neutrophils after interaction with nontypeable Haemophilus influenzae

Garofalo, Roberto P.; Faden, Howard; Sharma, Sharad; Ogra, Pearay L.

doi:10.1128/iai.59.11.4221-4226.1991

Cited by 10 publications

(4 citation statements)

References 27 publications

(20 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bacterial aggregation or bactericidal activity of the serum could have reduced the number of viable bacteria. Garofalo et al (11), measuring the association of radioactive labeled bacteria with PMNs at a bacterium/PMN ratio of 10:1, did not include controls to assess the viability of the bacteria, which we found to be an important factor. Heat-killed bacteria were opsonophagocytized better than viable bacteria (Fig.…”

Section: T Viable Killedmentioning

confidence: 85%

Quantitative flow cytometric analysis of opsonophagocytosis and killing of nonencapsulated Haemophilus influenzae by human polymorphonuclear leukocytes

Vogel

Alphen

Geluk

et al. 1994

Clin Diagn Lab Immunol

View full text Add to dashboard Cite

Since nonencapsulated Haemophilus influenzae persists in the lower respiratory tracts of patients with chronic bronchitis despite the presence of specific antibodies, complement, and polymorphonuclear leukocytes (PMNs), opsonophagocytosis of H. influenzae was analyzed. Nonencapsulated H. influenzae isolated from the sputa of chronic bronchitis patients was labeled with fluorescein isothiocyanate and incubated with human PMNs in the presence of complement and antibodies for 30 min at 37 degrees C. Candida albicans was added to each sample as an internal standard, and the reduction of the number of bacteria was determined by flow cytometry. Fluorescence quenching with ethidium bromide was used to discriminate between intracellular and extracellular bacteria. Opsonophagocytosis of viable H. influenzae d1 was 17% +/- 29% in the presence of complement and human pooled sera containing high titers of strain-specific antibodies. Opsonophagocytosis of six other H. influenzae strains was also poor. Under the same conditions, opsonophagocytosis of Staphylococcus aureus was 90% +/- 5%, and opsonophagocytosis of C. albicans was 55% +/- 23%. About half of the number of H. influenzae bacteria associated with PMNs was internalized. Opsonophagocytosis of heat-killed H. influenzae d1 (41% +/- 20%) was higher than that of viable bacteria of the same strain (P < 0.05). This result suggests that the accessibility of epitopes on H. influenzae for opsonizing antibodies is better on killed than on viable bacteria. We conclude that viable nonencapsulated H. influenzae is poorly opsonophagocytized in the presence of strain-specific antibodies and complement.

show abstract

Section: T Viable Killedmentioning

confidence: 85%

Quantitative flow cytometric analysis of opsonophagocytosis and killing of nonencapsulated Haemophilus influenzae by human polymorphonuclear leukocytes

Vogel

Alphen

Geluk

et al. 1994

Clin Diagn Lab Immunol

View full text Add to dashboard Cite

show abstract

“…Studies of the generation of eicosanoids in human blood after the phagocytosis of zymosan showed that LTB4 synthesis increased to maximum levels from 30 to 60 min after the stimulus and remained essentially constant thereafter for at least another hour (12). Also, increased LTB4 release by PMN parallels the kinetics of opsonic phagocytosis of H. influenzae in vitro (14). Finally, in an animal model of colonic inflammation, increased LTB4 synthesis correlated with PMN infiltration for a duration of several weeks (50).…”

Section: Discussionmentioning

confidence: 97%

Effector mechanisms of intestinally induced immunity to Pseudomonas aeruginosa in the rat lung: role of neutrophils and leukotriene B4

et al. 1993

View full text Add to dashboard Cite

This paper investigates the effector mechanisms of immune clearance in the lungs of rats immunized against mucoid Pseudomonas aeruginosa. After the gut-associated lymphoid tissue was primed and after a subsequent pulmonary challenge with live bacteria, significantly accelerated bacterial clearances from the lung and raised levels of anti-P. aeruginosa antibodies in sera (immunoglobulin G [IgG], IgA, and IgM) and bronchoalveolar lavages (IgG and IgA) were observed for all immune animals. These changes were associated with enhanced recruitment, chemotaxis, chemokinesis, phagocytic indices, and chemiluminescence of pulmonary polymorphonuclear neutrophils (PMN). In the alveolar spaces of immune animals, an increase in the level of PMN recruitment was not associated with higher levels of leukotriene B4 (LTB4). In contrast, in nonimmune animals that were intratracheally infected with P. aeruginosa, the levels of recruitment and activity of alveolar PMN were lower than those in immune rats but PMN infiltration correlated with a significant increase in the synthesis of LTBI4 in the alveolar space. In pulmonary tissue, LTB4 synthesis for both groups was elevated. These findings suggest that accelerated clearance of mucoid P. aeruginosa from the lungs of intestinally immunized rats is due at least in part to factors that induce the enhancement of PMN recruitment and activity in the alveolar space. The mediators that regulate this enhanced response remain unknown but do not seem to include LTB4. The high levels of LTB4 measured in the bronchoalveolar lavages and pulmonary tissues from nonimmune animals infected with live bacteria implicate LTB4 as an important amplifier of the inflammatory response during acute pulmonary infections with mucoid P. aeruginosa in unimmunized hosts.

show abstract

“…The necessity for complement in the phagocytic process is somewhat controversial. In our experience, phagocytosis can occur in the presence of antibody alone; however, complement augments the process 47 . In contrast, studies conducted by Troestra et al 46 and Musher et al 48 attribute greater importance to the role of complement.…”

Section: Igg‐specific Antibody Response (μG/ml) Of Children With Acutmentioning

confidence: 56%

“…In contrast, studies conducted by Troestra et al 46 and Musher et al 48 attribute greater importance to the role of complement. From our own studies, it is clear that phagocytosis is associated with the release of leukotriene B4, a potent chemoattractant for neutrophils 47 . Thus, the inflammatory response is heightened.…”

Section: Igg‐specific Antibody Response (μG/ml) Of Children With Acutmentioning

confidence: 94%

Immunology of the Middle Ear: Role of Local and Systemic Antibodies in Clearance of Viruses and Bacteria

Faden

1997

Annals of the New York Academy of Sciences

View full text Add to dashboard Cite

Acute otitis media may be defined as the sudden onset of inflammation in the middle-ear space characterized by ear pain and a bulging, thickened, and immobile tympanic membrane. The middle-ear fluid is typically purulent. A bacterial pathogen is recovered in 75% of the episodes. Streptococcus pneumoniae predominates at 35%, followed by nontypeable Hemophilus influenzae at 25%, and Moraxella catarrhalis at 15%. The remaining 25% of middle-ear fluids are sterile and presumably represent viral infections. Increasing evidence suggests that viruses may participate in more than 50% of all acute otitis media (AOM) episodes; however, pure viral AOM is relatively uncommon. IThe natural course of AOM has not been studied in the past decade. A review of articles concerning treatment of AOM demonstrates a spontaneous self-resolution rate between 30 and 80%.24 This rate varies with the specific pathogen from a high rate of 79% with M. catarrhalis to a low rate of 11% with S. pne~moniae.'.~ The mechanisms of eradication appear to involve various host defenses, including phagocytosis and destruction within neutrophils, lysis by host products outside of the cell, immunologic destruction by antibody and complement, and drainage of pus from the middle-ear space through the eustachian tube.Ample evidence has been accumulated that supports the role of serum antibody in protecting the middle-ear space from infection. For example, the incidence of AOM is relatively low during the first six months of life when maternally acquired antibody levels are relatively high in infants. More specifically, antibody concentrations to S. pneumoniae in cord blood correlate to susceptibility to the development of pneumococcal otitis media, as does the level of antibody to nontypeable H. influenzae in older Low levels of antibody can be corrected by passive immunization with serum containing high titers of antibody. By passively raising levels of pneumococcal antibody, the frequency of otitis media is reduced. I* Active immunization with S. pneumoniae has also been associated with a reduced frequency of

show abstract

Release of leukotriene B4 from human neutrophils after interaction with nontypeable Haemophilus influenzae

Cited by 10 publications

References 27 publications

Quantitative flow cytometric analysis of opsonophagocytosis and killing of nonencapsulated Haemophilus influenzae by human polymorphonuclear leukocytes

Quantitative flow cytometric analysis of opsonophagocytosis and killing of nonencapsulated Haemophilus influenzae by human polymorphonuclear leukocytes

Effector mechanisms of intestinally induced immunity to Pseudomonas aeruginosa in the rat lung: role of neutrophils and leukotriene B4

Immunology of the Middle Ear: Role of Local and Systemic Antibodies in Clearance of Viruses and Bacteria

Contact Info

Product

Resources

About