Release of Leukotriene C&lt;sub&gt;4&lt;/sub&gt; from Human Eosinophils and its Relation to the Cell Density

Kajita, Toshiyuki; Yui, Yasuo; Mita, Haruhisa; Taniguchi, Noboru; Saitô, Hiroshi; Mishima, Taketoshi; Shida, Takao

doi:10.1159/000233922

Cited by 86 publications

(43 citation statements)

References 11 publications

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“…Low density eosinophils obtained in vitro by incubation with GM-CSF are cytotoxic to IgGcoated schistosomula [65]. Furthermore, light density eosinophils from patients with HES generate and release greater amounts of leukotriene C 4 in response to IgG-or complement-coated targets and the monocyte-derived eosinophil-activating factor (EAF) than do normal density eosinophils [155,159,160]. Low density eosinophils from patients with HES show an increased deoxyglucose uptake and oxygen consumption and incorporation of PAF into the phospholipid pool than normodense cells [155,161,162] indicating a higher metabolic activity in light density eosinophils.…”

Section: Functional Heterogeneitymentioning

confidence: 99%

Pulmonary immune cells in health and disease: the eosinophil leucocyte (Part I)

Kroegel¹,

Virchow²,

Luttmann³

et al. 1994

Eur Respir J

129

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P Pu ul lm mo on na ar ry y i im mm mu un ne e c ce el ll ls s i in n h he ea al lt th h a an nd d d di is se ea as se e: : t th he e e eo os si in no op ph hi il l l le eu uc co oc cy yt te e ( (P Pa ar rt t I I) ) ABSTRACT: Increasing evidence has accumulated to suggest that eosinophils play a key role in the pathogenesis of asthma and other pulmonary diseases by damaging infiltrated bronchial tissue and lung parenchyma. The first part of this review on eosinophils describes the cellular characteristics and properties of the cell, which help in understanding its role in disease. The article focuses on origin, maturation and differentiation of the eosinophil, its morphological and phenotypical properties, as well as its preformed and newly generated mediators of inflammation. The cause and putative significance of eosinophil heterogeneity in respect to function and density will also be discussed. In addition, the naturally occurring mediators through which eosinophils are activated and communicate with other inflammatory cells are outlined.The first part closes with new aspects of eosinophil recruitment from the circulation into perivascular tissue, including nonselective and putative selective adhesion mechanisms and chemotaxis.

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Section: Functional Heterogeneitymentioning

confidence: 99%

Pulmonary immune cells in health and disease: the eosinophil leucocyte (Part I)

Kroegel¹,

Virchow²,

Luttmann³

et al. 1994

Eur Respir J

129

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“…Hypodense eosinophils, defined by their lower than normal sedimentation density in discontinuous metrizamide or Percoll gradients, have been found in the peripheral blood of some patients with eosinophilia-associated diseases such as the idiopathic hypereosinophilic syndrome and chronic infection with S. mansoni (3)(4)(5)(6). These eosinophils generate more LTC4 in response to activation with calcium ionophore (7) and kill more antibody-coated S. mansoni larvae (5) than normodense eosinophils.…”

Section: Introductionmentioning

confidence: 99%

Human eosinophils have prolonged survival, enhanced functional properties, and become hypodense when exposed to human interleukin 3.

Rothenberg

Owen²,

Silberstein³

et al. 1988

J. Clin. Invest.

450

169

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“…Whereas normodense eosinophils are the predominant physical phenotype found in the peripheral blood of healthy individuals, variable numbers of hypodense eosinophils are found in the blood of patients with eosinophil-associated diseases, such as the idiopathic hypereosinophilic syndrome (IHES) (2)(3)(4). This physical diversity is associated with functional heterogeneity (4)(5)(6)(7)(8). In comparison to normodense eosinophils, hypodense eosinophils exhibit transiently enhanced ex vivo survival attributable to exposure to eosinophilopoietic cytokines in vivo (4).…”

mentioning

confidence: 99%

Constitutive production of granulocyte/macrophage colony-stimulating factor by hypodense mononuclear eosinophils developed in vitro from hybrid eosinophil/basophil granulocytes.

Boyce¹,

Friend²,

Gurish³

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

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We recently described the development in vitro of cells with granules characteristic of eosinophils and basophils (hybrid granulocytes) from normal human cord blood mononuclear cells cultured for 14 days with recombinant human (rh) interleukin (IL)-3, rhIL-5, and a soluble basement membrane, Matrigel. Hybrid granulocytes constitutively produced granulocyte/macrophage colony-stimulating factor (GM-CSF) and rapidly developed into eosinophils after the exogenous cytokines and Matrigel were removed. To characterize the developmental progression of hybrid granulocytes, cells were maintained for an additional 14 days in medium containing rhIL-3, rhIL-5, and Matrigel. After 28 days, 73% ± 1% (mean + SEM; n = 6) of the nonadherent cells were mononuclear eosinophils, 13% + 3% were eosinophils with two or more nuclear lobes, 13% + 4% were hybrid granulocytes, and 0.2% ± 0.1% were basophils. More than 90%Yo of the mononuclear eosinophils were hypodense as determined by centrifugation through metrizamide gradients. After an additional 5 days of culture in medium without exogenous cytokines, 65% + 3% (n = 5) of the 28-day cells excluded trypan blue. In contrast, 2% ± 1% of freshly isolated peripheral blood eosinophils survived 5 days of culture without exogenous cytokines (n = 5). Fifty percent conditioned medium from in vitro derived 28-day mononuclear eosinophils and 14-day hybrid granulocytes maintained the survival of 60% + 7% and 77% + 7%, respectively, of freshly isolated peripheral blood eosinophils for 72 h, compared with 20%o + 8% survival in medium alone (n = 3). The eosinophil viabilitysustaining activity of 50%o mononuclear eosinophil-conditioned medium was neutralized with a GM-CSF antibody. A total of 88% of the 28-day cells exhibited immunochemical staining for GM-CSF. Thus, during eosinophilopoiesis, both hybrid eosinophil/basophil intermediates and immature mononuclear eosinophils exhibit autocrine regulation of viability due to constitutive production of GM-CSF.When centrifuged through discontinuous gradients of Percoll or metrizamide, peripheral blood eosinophils segregate into populations of normal (normodense) or relatively less (hypodense) centrifugation density (1). Whereas normodense eosinophils are the predominant physical phenotype found in the peripheral blood of healthy individuals, variable numbers of hypodense eosinophils are found in the blood of patients with eosinophil-associated diseases, such as the idiopathic hypereosinophilic syndrome (IHES) (2-4). This physical diversity is associated with functional heterogeneity (4-8). In comparison to normodense eosinophils, hypodense eosinophils exhibit transiently enhanced ex vivo survival attributable to exposure to eosinophilopoietic cytokines in vivo (4). Both normodenseThe publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact. and hypodense peripheral blood eosinophils maintain their viabi...

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Release of Leukotriene C<sub>4</sub> from Human Eosinophils and its Relation to the Cell Density

Cited by 86 publications

References 11 publications

Pulmonary immune cells in health and disease: the eosinophil leucocyte (Part I)

Pulmonary immune cells in health and disease: the eosinophil leucocyte (Part I)

Human eosinophils have prolonged survival, enhanced functional properties, and become hypodense when exposed to human interleukin 3.

Constitutive production of granulocyte/macrophage colony-stimulating factor by hypodense mononuclear eosinophils developed in vitro from hybrid eosinophil/basophil granulocytes.

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