Release of lysosomal enzymes in experimental inflammations; Effects of anti-inflammatory drugs

Effects of Steroid Hormones on Human Polymorphonuclear Leukocyte Lysosomes

“…This association with anti-inflammatory corticosteroids has also been observed with in vivo models of inflammation (27,28) and lysosome rupture (29). But direct membrane interaction by these compounds and resulting stabilization need not be invoked to explain their antiphlogistic actions.…”

The mechanism of action of a single dose of methylprednisolone on acute inflammation in vivo.

“…Ward reported inhibition of chemotaxis by hydrocortisone (8), while Borel reported no effect (9). Inflammatory area perfusates from rat paws inj ected with nystatin have shown increased levels of lysosomal enzymes that are markedly reduced by neutrophil-depleting methotrexate pretreatment or steroids (11). No simultaneous quantitative studies of in vivo chemotaxis were made in this model.…”

“…(11) ENIC VN, volume of fluid for sponge pool N. WCEE, the white cell equivalent of the enzyme content of supernatant fluid (number of cells) is a derived parameter. It is a theoretical estimate of the number of white cells whose death and lysis would be required to provide the excess enzyme found in supernatant fluids of control rats when compared to agranulocytic rats.…”

“…A perfusion technique was then used to collect inflammatory fluid for enzyme assays (11). Dexamethasone (100 Ag/kg, orally) and beta methasone (100 ytg/kg, orally) decreased lysosomal enzyme levels in perfusates.…”

Characterization of Inhibitor(s) of Lymphocyte Activation in Serum from Rats with Adjuvant Arthritis

“…It begins to appear a few days later, before the secondary lesions become apparent. In the serum of animals with nystatin-induced edema, an inflammatory reaction due to a local release of lysosomal enzymes (30), no inhibitory activ ity has been found. In Sprague-Dawley rats, which show large inter-individual variations in the intensity of the delayed systemic response to the adjuvant (31), the inhibitory activity is correlated with the intensity of the secondary reaction.…”