Release of Mitochondrial and Nuclear DNA During On-Pump Heart Surgery: Kinetics and Relation to Extracellular Vesicles

Abstract

(UiO) in 2015. There I have met many fantastic people whose openness, enthusiasm, and disposition toward risky investments made this work possible. I will first greatly acknowledge Professor Jarle Vaage for inviting me to UiO and being my permanent co-supervisor. Thank you for invaluable help and for sharing your realistic optimism during all these exciting and challenging years. I am especially thankful for introducing me to Professor Guro Valen. Professor Guro Valen was a bright and inspiring supervisor under whose guidance we charted the blueprint of this work. She passed away in September 2014. I see no better way to express gratitude to her than being an active researcher. Professor Kåre-Olav Stensløkken has navigated me through the most challenging part of Ph.D., the final one. Thank you for the wise combination of understanding, proactive motivation, and especially for your patience! The triumvirate of Guro, Jarle, and Kåre-Olav fostered a unique working environmentour research group was always international, open, inclusive, and creative. It is my pleasure to name my colleagues from whom I have learned a lot: Arkady Rutkovsky, Lars Henrik Mariero, Marte Bliksøen, Apple Lei, and Fred Haugen were those with whom I have worked most with. Many thanks to doctor Rutkovsky for informal and all-purpose guidance during my first years in Norway. My special thanks to Torun Flatebø for numerous Westerns and PCRs you run for me. I would also like to give a tribute to all my previous colleagues:

Minimized Extracorporeal Circulation Is Associated with Reduced Plasma Levels of Free-Circulating Mitochondrial DNA Compared to Conventional Cardiopulmonary Bypass: A Secondary Analysis of an Exploratory, Prospective, Interventional Study

“…While the absolute plasma levels of mtDNA during cCPB were comparable with other studies investigating CABG patients, the kinetics differed from some studies [ 17 , 18 ]. Nevertheless, they were also aggregable with another investigation [ 28 ]. The studies by Qin et al, also showed an early mtDNA release, which, however, did not peak until 12 h after surgery [ 17 , 18 ].…”

Cell-Free Circulating Mitochondrial DNA: A Potential Blood-Based Marker for Atrial Fibrillation

“…In addition, (mildly) damaged mitochondria may be incorporated in exosomes, which translocate to the extracellular environment. Here, the mtDNA is released and end up in the circulation, forming cfc-mtDNA [42,43]. The presence of mitochondrial DNA in the media of rapid electrical-stimulated cardiomyocytes confirmed that mitochondrial DNA can be released out of the cardiomyocytes into the circulation.…”

Next generation sequencing‑based analysis of mitochondrial DNA characteristics in plasma extracellular vesicles of patients with hepatocellular carcinoma

“…Consistent with the protective role of EVs, EV DNA had a slightly higher copy number compared with cfDNA. A recent study also showed a higher concentration of mtDNA in EVs compared with plasma ( 37 ). The present study also confirmed that EV DNA covered the whole mitochondrial genome, suggesting that EV mtDNA can carry all the information from tumor cells and may be used as a potential cancer biomarker.…”