Release of potentially fermentable sugars during dilute acid treatments of Bermuda grass NK37 (<i>Cynodon dactylon)</i>for second-generation ethanol production

Canizo, Jésica; Cortés-Callejas, M. Luisa; Davila-Gomez, Francisco J.; Heredia‐Olea, Erick; Pérez‐Carrillo, Esther; Serna‐Saldívar, Sergio O.

doi:10.1002/jctb.4280

Cited by 11 publications

(9 citation statements)

References 29 publications

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“…It was reported that Bermuda grass contains between 178 and 226 g/kg of xylans (Lee et al, 2009;Canizo et al, 2014), 304 g/kg of cellulose, 312 g/kg of glucans (Canizo et al, 2014), and 104 g/kg of CP (NRC, 2001). It has been shown that the xylan and cellulose contents in sorghum cultivars were between 142 and 177 g/kg (Dien et al, 2009;Stefaniak et al, 2012) and between 197 and 277 g/kg (Dien et al, 2009;Zhao et al, 2009) on a DM basis, respectively.…”

Section: Discussionmentioning

confidence: 99%

Nutrient value of spray field forages fed to pigs and the use of feed enzymes to enhance nutrient digestibility

Passos

Andrade

Phillips

et al. 2015

Journal of Animal Science

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This study determined the DE, ME, apparent total tract digestibility (ATTD) of N, and N retention of spray field forages (Bermuda grass, forage sorghum, and sweet sorghum) fed to pigs and the effects of the supplemental feed enzymes on energy and N utilization. A basal diet was formulated with 96% corn and 4% amino acids, minerals, and vitamins. Test diets contained 85% basal diet + 15% Bermuda grass, forage sorghum, or sweet sorghum. Allzyme SSF (Alltech, Nicholasville, KY) was used as a feed enzyme, which was composed of cellulase, glucanase, xylanase, phytase, and protease. The basal diet and test diets were evaluated by using 4 sets of 2 × 2 Latin square designs consisting of 2 pigs and 2 periods with a total of 32 barrows (38.7 ± 7.9 kg). Each period (10-d adjustment and 4-d collection) had 2 Latin squares. The 2 treatments were levels of enzyme supplementation (0 or 200 mg/kg). Pigs received experimental diets twice daily (0700 and 1700 h) at a fixed amount based on BW of pigs (0.09 × BW0.75 kg). On d 10, chromic oxide (0.5%) was added to the diets at 1700 h as an external marker. Fecal and urine samples were collected during 4 consecutive days. The basal diet contained 3,850 kcal DE/kg, 3,769 kcal ME/kg, 86.06% ATTD of N, and 71.10% N retention and was not affected by enzyme supplementation. Bermuda grass contained 893 kcal DE/kg, 845 kcal ME/kg, -16.50% ATTD of N, and -37.49% N retention and tended to be improved by enzyme supplementation to 1,211 kcal DE/kg (P = 0.098), 1,185 kcal ME/kg (P = 0.081), and -10.54% N retention (P = 0.076). The ATTD of N of Bermuda grass increased (P < 0.05) to 0.08% by enzyme supplementation. The forage sorghum contained 1,520 kcal DE/kg, 1,511 kcal ME/kg, -0.72% ATTD of N, and -16.99% N retention. The sweet sorghum contained 1,086 kcal DE/kg, 1,061 kcal ME/kg, -75.47% ATTD of N, and -49.22% N retention. Enzyme supplementation did not improve energy digestibility of forage sorghum and sweet sorghum. Nitrogen in these forages was poorly utilized. In conclusion, spray field forages including Bermuda grass, forage sorghum, and sweet sorghum can partly be utilized in pig feed to provide energy, although N is rather poorly digested. Feed enzymes could enhance both energy and N utilization in Bermuda grass but not sorghum.

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Section: Discussionmentioning

confidence: 99%

Nutrient value of spray field forages fed to pigs and the use of feed enzymes to enhance nutrient digestibility

Passos

Andrade

Phillips

et al. 2015

Journal of Animal Science

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“…According to Lee et al [21], the Bermuda grass contains 30.4, 22.6, 4.9 and 23.2% of glucans, xylans, arabinans and lignin, respectively. On the other hand, Canizo [22] determined a similar composition of the potentially fermentable sugars but a slightly lower lignin content ( Table 1).…”

Section: Fiber Compositionmentioning

confidence: 91%

“…Production of ethanol from physically, chemically and/or enzymatically treated hydrolysates is feasible with the utilization of osmotolerant and C5 (pentose) yeast or bacterial strains. The recent advances in biotechnology has generated numerous genetically modified or engineered yeast and bacteria proficient of fermenting hydrolyzates containing significant amounts of With the estimated ethanol yield obtained by Canizo et al [22] is feasible to attain a theoretical amount of 180 mg of ethanol g −1 of dried Bermuda grass. This means a theoretical ethanol yield between 1.08 and 4.86 t ha −1 year −1 (Figure 1).…”

Section: Enzymatic Hydrolysis and Fermentationmentioning

confidence: 99%

“…Likewise, Ballesteros et al[35] obtained 16.2 g ethanol L −1 (10% solids) from sweet sorghum bagasse hydrolyzates fermented with K. marxianus, whereas Kurian et al[29] working with P. stipitis produced 38.7 g ethanol L −1 with a theoretical conversion of 82.5%.Although similar pretreatments have been also applied to Bermuda grass (alkaline, hot water, ozonolysis, steam explosion), the diluted acid hydrolysis had better effectiveness in terms of releasing monomeric sugars, specially xylose[23]. Canizo et al[22] concluded that H 2 SO 4 hydrolysis (121°C, 75 min, 1.25% acid concentration, and 12.5 solid to liquid fraction) released 20.9% glucose, 66.6% xylose and 90% arabinose. Likewise, Ballesteros et al[35] used a hydrolysis scheme with more acid concentration (1.5% w/w) and time (90 min) achieving 66 and 52% xylose and glucose yield, respectively.…”

mentioning

confidence: 99%

“…A significant improvement in fermentable carbohydrate yield from Bermuda grass is documented after using a fiber-degrading enzyme complex[23]. Likewise, Canizo et al[22] hydrolyzed Bermuda grass solids after separating the acid pretreated hydrolysate using 27 FPU celullase g −1 cellulose, 0.6% β-glycosidase and 0.5% xylanase for 168 h at 50°C, pH 4.8 and 150 rpm and concluded that the dual technology released 90.1% of glucose from cellulose and 18% xylose from hemicellulose. Thus, the dual treatment yielded 89.2% of the total sugars from the Bermuda grass cell walls (Table 2).…”

mentioning

confidence: 99%

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Conversion of High Biomass/Bagasse from Sorghum and Bermuda Grass into Second-Generation Bioethanol

Heredia‐Olea¹,

Serna‐Saldívar²,

Pérez‐Carrillo³

et al. 2018

Biofuels - State of Development

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Sorghum (Sorghum bicolor) and Bermuda (Cynodon dactylon) grass are examples of annual and perennial forage crops produced throughout the globe. These crops should be harvested at the peak of biomass production when the levels of lignin are relatively low. The high biomass sorghum, sweet sorghum bagasse (2 cuts or crops year −1 ) or Bermuda grass capable of yielding up to 50, 60 and 27 tons of dry forage ha −1 year −1 rich in cellulose and hemicellulose can be efficiently transformed into bioethanol using second-generation technologies consisting of milling, pretreatment (chemical and/or enzymatic) and fermentation with microorganisms capable of transforming C5/C6 sugars to obtain ethanol. An alternative process contemplates the extrusion aimed toward the physical disruption of cell walls minimizing the use of considerable amounts of water and chemicals commonly used during pretreatment. Extruded feedstocks treated with fiber-degrading enzyme cocktails had conversion efficiencies between 60 and 78% of the hemicellulose and cellulose similar to the ones achieved after acid/enzyme hydrolyses. The chief advantages of this continuous process are that hydrolysates are practically free of enzymes and yeast inhibitors. These feedstocks can produce up to 310 L anhydrous bioethanol dry t −1 and have a great potential for widespread use.

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Enhanced production of xylose from corncob hydrolysis with oxalic acid as catalyst

Jin

Zhao

Liu

et al. 2017

Bioprocess Biosyst Eng

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The acid-catalyzed treatment was a conventional process for xylose production from corncob. To increase the release of xylose and to reduce the by-products formation and water usage, the oxalic acid was used as catalyst to hydrolyze the corncob and the hydrolytic conditions were investigated. The highest xylose yield of 32.7 g L, representing 96.1% of total theoretical xylose yield, was obtained using 1.2% oxalic acid after hydrolysis for 120 min at 130 °C, which was more than 10% higher than that of sulfuric acid-catalyzed hydrolysis. Mixed acids-catalyzed hydrolysis performed a synergistic effect for xylose production and 31.7 g L of xylose was reached after reacting for 90 min with oxalic acid and sulfuric acid at a ratio of 1:4 (w/w). A kinetic model was developed to elucidate the competitive reaction between xylose formation and its degradation in the hydrolysis process, and the experimental data obtained in this study were perfectly in agreement with that of predicted from the model. Furthermore, the final xylose yield of 85% was achieved after purification and crystallization. It was demonstrated that xylose production from the corncob hydrolysis with oxalic acid as the catalyst was an effective alternative to the traditional sulfuric acid-based hydrolysis.

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Release of potentially fermentable sugars during dilute acid treatments of Bermuda grass NK37 (Cynodon dactylon)for second-generation ethanol production

Cited by 11 publications

References 29 publications

Nutrient value of spray field forages fed to pigs and the use of feed enzymes to enhance nutrient digestibility

Nutrient value of spray field forages fed to pigs and the use of feed enzymes to enhance nutrient digestibility

Conversion of High Biomass/Bagasse from Sorghum and Bermuda Grass into Second-Generation Bioethanol

Enhanced production of xylose from corncob hydrolysis with oxalic acid as catalyst

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