Release of vancomycin from multilayer coated absorbent gelatin sponges

Shukla, Anita; Fang, Jean C.; Puranam, Sravanthi; Hammond, Paula T.

doi:10.1016/j.jconrel.2011.09.062

Cited by 69 publications

(34 citation statements)

References 42 publications

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“…A few studies used amphiphiles to disperse SWNT within Langmuir-Blodgett films [64][65][66], but ours appears to be the first application to LbL films. A few reports of LbL strategies toward antimicrobial films have appeared, with the antimicrobial effect based on cationic polymers [17], silver nanoparticles [67], and various antibiotics [68][69][70]. However, approaches involving SWNT as the antimicrobial agent within an LbL film are rather rare.…”

Section: Discussionmentioning

confidence: 99%

Carbon nanotube-based antimicrobial biomaterials formed via layer-by-layer assembly with polypeptides

Aslan

Deneufchatel

Hashmi

et al. 2012

Journal of Colloid and Interface Science

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Section: Discussionmentioning

confidence: 99%

Carbon nanotube-based antimicrobial biomaterials formed via layer-by-layer assembly with polypeptides

Aslan

Deneufchatel

Hashmi

et al. 2012

Journal of Colloid and Interface Science

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“…102 Gelatin sponge is a surgical haemostatic sponge synthesized from highly purified gelatin and is used in cases where traditional hemostasis is impractical or difficult and use of other nonabsorbable material is undesirable. 103 Gelatin can absorb 45 times its weight of its initial value and hence exhibit excellent hemostatic ability. Its disadvantage is that it undergoes excessive swelling, which can lead to the risk of nerve compression.…”

Section: Proteinsmentioning

confidence: 99%

Natural Polymer/Inorganic Material Based Hybrid Scaffolds for Skin Wound Healing

et al. 2015

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Dermal tissue engineering focuses on the restoration of diseased and damaged tissues by using a combination of cells, biomaterials, and bioactive molecules. Inorganic substances like zeolites, clay, mesoporous silica, metals, and metal oxides are advanced materials used in wound healing research. They can improve the structural stability and bioactivity of bio polymeric scaffolds. Zeolites, clays, and mesoporous silica act as suitable carriers for drug delivery and when incorporated within scaffolds, serve as ideal matrices for promoting skin regeneration. This review focuses on various natural polymers/inorganic materials based composite scaffolds used for skin tissue engineering, highlighting their synthesis routes and mode of action by which wound healing is enhanced. Among the different inorganic materials used, the role of zeolites incorporated biocomposites for promoting blood coagulation, antibacterial effect; oxygen delivery to cells and wound healing are discussed in detail. The article thus includes recent attempts to explore the hidden potential of inorganic materials in dermal tissue engineering.

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“…31 The electrostatically anchored multilayer structure provided a controlled release of the loading cargo, which is pivotal for local drug delivery in order to obtain a sustained effect. [32][33][34] H1299 originates from epithelial lung cancer and has a prominent ability of endocytosis. Therefore, it is an ideal cell line for validating CS parameters for siRNA delivery.…”

Section: Discussionmentioning

confidence: 99%

Chitosan/siRNA functionalized titanium surface via a layer-by-layer approach for in vitro sustained gene silencing and osteogenic promotion

Song¹,

Song²,

Yang³

et al. 2015

IJN

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Titanium surface modification is crucial to improving its bioactivity, mainly its bone binding ability in bone implant materials. In order to functionalize titanium with small interfering RNA (siRNA) for sustained gene silencing in nearby cells, the layer-by-layer (LbL) approach was applied using sodium hyaluronate and chitosan/siRNA (CS/siRNA) nanoparticles as polyanion and polycation, respectively, to build up the multilayered film on smooth titanium surfaces. The CS/siRNA nanoparticle characterization was analyzed first. Dynamic contact angle, atomic force microscopy, and scanning electron microscopy were used to monitor the layer accumulation. siRNA loaded in the film was quantitated and the release profile of film in phosphate-buffered saline was studied. In vitro knockdown effect and cytotoxicity evaluation of the film were investigated using H1299 human lung carcinoma cells expressing green fluorescent protein (GFP). The transfection of human osteoblast-like cell MG63 and H1299 were performed and the osteogenic differentiation of MG63 on LbL film was analyzed. The CS/siRNA nanoparticles exhibited nice size distribution. During formation of the film, the surface wettability, topography, and roughness were alternately changed, indicating successful adsorption of the individual layers. The scanning electron microscope images clearly demonstrated the hybrid structure between CS/siRNA nanoparticles and sodium hyaluronate polymer. The cumulated load of siRNA increased linearly with the bilayer number and, more importantly, a gradual release of the film allowed the siRNA to be maintained on the titanium surface over approximately 1 week. In vitro transfection revealed that the LbL film-associated siRNA could consistently suppress GFP expression in H1299 without showing significant cytotoxicity. The LbL film loading with osteogenic siRNA could dramatically increase the osteogenic differentiation in MG63. In conclusion, LbL technology can potentially modify titanium surfaces with specific gene-regulatory siRNAs to enhance biofunction.

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Release of vancomycin from multilayer coated absorbent gelatin sponges

Cited by 69 publications

References 42 publications

Carbon nanotube-based antimicrobial biomaterials formed via layer-by-layer assembly with polypeptides

Carbon nanotube-based antimicrobial biomaterials formed via layer-by-layer assembly with polypeptides

Natural Polymer/Inorganic Material Based Hybrid Scaffolds for Skin Wound Healing

Chitosan/siRNA functionalized titanium surface via a layer-by-layer approach for in vitro sustained gene silencing and osteogenic promotion

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