Releasing and structural/mechanical properties of nano-particle/Punica granatum (Pomegranate) in poly(lactic-co-glycolic) acid/fibrin as nano-composite scaffold

Gorji, Mona; Zargar, A.; Setayeshmehr, Mohsen; Ghasemi, Negin; Soleimani, Mitra; Kazemi, Mohammad Hossein; Hashemibeni, Batool

doi:10.4149/bll_2021_007

Cited by 4 publications

(3 citation statements)

References 41 publications

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“…To solve the issue, PLGA with a proper degradation period (3-6 months) can be a promising substance. PLGA is most widely used as a biomaterial for biomedical applications thanks to its superior biocompatibility and biodegradation properties, supporting cell attachment, anchorage, and proliferation [31] [32] [33]. The degradability of this copolymer results from its chemical aspects making its hydrolytic degradation possible [34].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of 3D-printed quaternary scaffolds containing polymeric matrix plus alginate nanoparticles and MWCNTs for cartilage tissue engineering

Pourmollaabbassi

Mahdavi

Shojaei

et al. 2023

Preprint

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Over the years, articular cartilage damage has impacted living standards world wide. Since each of the traditional therapeutic approaches has limitations, tissue engineering-based approaches have been recruited to provide a feasible solution. This study aimed to develop a novel nanocomposite 3D printed scaffold via a polymeric matrix accompanied with alginate nanoparticles and functionalized multi-walled carbon nanotubes (MWCNTs) to investigate its potential appropriateness for cartilage tissue engineering application. In this way, 3D printed constructs was developed by an extrusion-based printing method using the innovative nanocomposite inks consisting of PCL (polycaprolactone, P 35% w/v) and PLGA (poly (lactic-co-glycolic acid, P 15% w/v) incorporated with alginate nanoparticles (40 and 45% w/v), as a filler, and modified with or without MWCNTs (0.05 and 0.1% w/v), as a composite reinforcement. Next, the characterization of scaffold features was investigated. Results revealed that 3D printed scaffold containing PP/alginate45% with MWCNT0.05 (PPA45M0.05) had significant improvements in porosity (74.29%±7.33), water uptake, absorbance, cell attachment, hydrophilicity (64.15 ± 1.87), the compression modulus(0.2174MPa), and the degradation rate. In addition, the interaction within the whole constituents was validated by the spectra of ATR-FTIR. Due to the proper biodegradability, biocompatibility, and mechanical aspects, the PPA45M0.05 scaffolds would be a potential construct for cartilage tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Fabrication of 3D-printed quaternary scaffolds containing polymeric matrix plus alginate nanoparticles and MWCNTs for cartilage tissue engineering

Pourmollaabbassi

Mahdavi

Shojaei

et al. 2023

Preprint

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“…34,35 Recent studies have also investigated fibrin-loaded stem cells, such as bone marrow mononuclear cells and mesenchymal stem cells. 36–39…”

Section: Introductionmentioning

confidence: 99%

“…34,35 Recent studies have also investigated fibrin-loaded stem cells, such as bone marrow mononuclear cells and mesenchymal stem cells. [36][37][38][39] In this study, we aimed to design a cornea-like scaffold to facilitate transplantation for the treatment of corneal injury. Our scaffold is composed of readily accessible PFMs that are loaded with BMSCs and corneal epithelial cells (Fig.…”

Section: Introductionmentioning

confidence: 99%

Plasma fibrin membranes loaded with bone marrow mesenchymal stem cells and corneal epithelial cells promote corneal injury healing via attenuating inflammation and fibrosis after corneal burns

2023

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A modular approach to 3D-printed bilayer composite scaffolds for osteochondral tissue engineering

Maherani,

Eslami,

Poursamar

et al. 2024

J Mater Sci: Mater Med

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Prolonged osteochondral tissue engineering damage can result in osteoarthritis and decreased quality of life. Multiphasic scaffolds, where different layers model different microenvironments, are a promising treatment approach, yet stable joining between layers during fabrication remains challenging. To overcome this problem, in this study, a bilayer scaffold for osteochondral tissue regeneration was fabricated using 3D printing technology which containing a layer of PCL/hydroxyapatite (HA) nanoparticles and another layer of PCL/gelatin with various concentrations of fibrin (10, 20 and 30 wt.%). These printed scaffolds were evaluated with SEM (Scanning Electron Microscopy), FTIR (Fourier Transform Infrared Spectroscopy) and mechanical properties. The results showed that the porous scaffolds fabricated with pore size of 210–255 µm. Following, the ductility increased with the further addition of fibrin in bilayer composites which showed these composites scaffolds are suitable for the cartilage part of osteochondral. Also, the contact angle results demonstrated the incorporation of fibrin in bilayer scaffolds based on PCL matrix, can lead to a decrease in contact angle and result in the improvement of hydrophilicity that confirmed by increasing the degradation rate of scaffolds containing further fibrin percentage. The bioactivity study of bilayer scaffolds indicated that both fibrin and hydroxyapatite can significantly improve the cell attachment on fabricated scaffolds. The MTT assay, DAPI and Alizarin red tests of bilayer composite scaffolds showed that samples containing 30% fibrin have the more biocompatibility than that of samples with 10 and 20% fibrin which indicated the potential of this bilayer scaffold for osteochondral tissue regeneration. Graphical Abstract

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Releasing and structural/mechanical properties of nano-particle/Punica granatum (Pomegranate) in poly(lactic-co-glycolic) acid/fibrin as nano-composite scaffold

Cited by 4 publications

References 41 publications

Fabrication of 3D-printed quaternary scaffolds containing polymeric matrix plus alginate nanoparticles and MWCNTs for cartilage tissue engineering

Fabrication of 3D-printed quaternary scaffolds containing polymeric matrix plus alginate nanoparticles and MWCNTs for cartilage tissue engineering

Plasma fibrin membranes loaded with bone marrow mesenchymal stem cells and corneal epithelial cells promote corneal injury healing via attenuating inflammation and fibrosis after corneal burns

A modular approach to 3D-printed bilayer composite scaffolds for osteochondral tissue engineering

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