Relevance and Applicability of a Simple Earthworm Biomarker of Copper Exposure. II. Validation and Applicability under Field Conditions in a Mesocosm Experiment withLumbricus rubellus

Svendsen, Claus; Weeks, Jason M.

doi:10.1006/eesa.1996.1492

Cited by 46 publications

(30 citation statements)

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“…In a laboratory study employing L. rubellus they observed a significant reduction in the neutral-red retention time (NRR time), a measure of the lysosomal membrane stability of the coelomocytes, with increasing external copper concentrations. A reduced NRR time with increased soil copper concentrations was also observed in a field study with L. rubellus (Svendsen and Weeks 1997b), and for E. veneta and E. fetida exposed to copper and nickel, respectively (Scott-Fordsmand et al 1998). The NRR times showed a sigmoid dose-response curve when related to the external metal concentrations and clear threshold responses when related to the internal metal levels, above which the NRR times were totally depressed (Svendsen and Weeks 1997a,b;Scott-Fordsmand et al in press).…”

Section: Lysosomal Membrane Integritymentioning

confidence: 71%

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Biomarkers in Earthworms

Scott-Fordsmand¹,

Weeks²

2000

Reviews of Environmental Contamination and Toxicology

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Section: Lysosomal Membrane Integritymentioning

confidence: 71%

“…Plastic fire pollution L. castaneus Svendsen et al (1996) Cu E. andrei Svendsen and Weeks (1997a) Heavy metals L. rubellus Svendsen and Weeks (1997b) Plastic fire L. castaneus Svendsen et al (1998) Cu L. rubellus Weeks and Svendsen (1996) '(Car). carbamate.…”

Section: Scott-fordsmand Et Al (In Press)mentioning

confidence: 98%

Biomarkers in Earthworms

Scott-Fordsmand¹,

Weeks²

2000

Reviews of Environmental Contamination and Toxicology

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“…Physiological regulation by the organism has been shown to be a mechanism that can regulate internal concentrations of essential metals, such as copper, nickel, and zinc when the concentrations in the soil are high (e.g., Ireland, 1979;Fleckenstein and Graff, 1982;Svendsen and Weeks, 1997;Peijnenburg et al, 1999a). Total mercury and MMHg tissue concentrations were greatest in the earthworms exposed to soils at the higher mercury concentrations.…”

Section: Bioaccumulation Of T-hg and Mmhgmentioning

confidence: 97%

Bioaccumulation of Total Mercury and Monomethylmercury in the Earthworm Eisenia Fetida

Burton

Turley

Fisher

et al. 2006

Water Air Soil Pollut

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Standard Form 298 (Rev. 8/98) REPORT DOCUMENTATION PAGEPrescribed by ANSI Std. Z39.18 Form Approved OMB No. 0704-0188The public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing the burden, to Department of Defense, Washington Headquarters Services, Directorate for Information Operations and Reports (0704-0188), 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to any penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number.

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“…In addition, earthworms are cost-effective, they represent an animal model that is non-controversial and they are known to accumulate organic pollutants and metals from their surrounding soil environment via passive absorption and intestinal uptake (e.g. Haimi et al, 1992;Belfroid et al, 1993Belfroid et al, , 1995Goven et al, 1994;Svendsen and Weeks, 1997b).…”

Section: Introductionmentioning

confidence: 99%

Qualitative high field 1H-NMR spectroscopy for the characterization of endogenous metabolites in earthworms with biochemical biomarker potential

et al. 2005

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This study was designed to provide a database of the endogenous metabolites in earthworm extracts of the species, Eisenia veneta and Lumbricus terrestris by high resolution 1 H-NMR spectroscopy in view of identifying biomarkers of toxicity or stress in environmental metabolomics studies. 1D and 2D NMR spectroscopic techniques enabled the identification and confirmation of the organic components in the tissue extracts of whole and segmented earthworms, dissected organs, and coelomic fluid. The extracts gave rise to characteristic 1 H-NMR spectral fingerprints of the low MW metabolites contained, specific to the species of earthworm, and to the specific regions or dissected organs of the earthworms under investigation. Distinct changes in the normal biochemistry were observed upon starvation and cooling, such as markedly decreased glucose and maltose, but increased lactate, acetate, succinate, formate and acetone. Additionally, slightly decreased threonine, arginine, lysine, leucine, citrate, asparagine and glycine were observed. Furthermore, lactate could be identified as a biomarker of acute toxic stress in expressed coelomic fluid following exposure to a model ecotoxin (3-trifluoromethylaniline). This work supports the application of 1 H-NMR spectroscopy for the study of changes in the normal invertebrate biochemistry in order to allow for the reliable assessment of biomarker responses following toxicity testing.

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Relevance and Applicability of a Simple Earthworm Biomarker of Copper Exposure. II. Validation and Applicability under Field Conditions in a Mesocosm Experiment withLumbricus rubellus

Cited by 46 publications

References 20 publications

Biomarkers in Earthworms

Biomarkers in Earthworms

Bioaccumulation of Total Mercury and Monomethylmercury in the Earthworm Eisenia Fetida

Qualitative high field 1H-NMR spectroscopy for the characterization of endogenous metabolites in earthworms with biochemical biomarker potential

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