Relevance of Animal Models for Studies of Immune Regulation of Atopic Allergy

Björkstén, Bengt; Ahlstedt, S.

doi:10.1111/j.1398-9995.1984.tb01949.x

Cited by 16 publications

(7 citation statements)

References 60 publications

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“…For the study on allergic diseases, several experimental models showing type I reaction or eosinophilic inflammation have been developed in mice, rats, and guinea pigs by using Ascaris, ovalbumin, and other allergens [20]. In addition, several studies showed that active immunization of monkeys with pollen allergens including recombinant Bet v 1 and Bet v 2 induced antigen-specific IgE production and some allergic symptoms [8,9].…”

Section: Discussionmentioning

confidence: 99%

Experimental Monkey Model Sensitized with Mite Antigen

Yasue

Nakamura

Yokota

et al. 1998

Int Arch Allergy Immunol

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Background: Monkeys are considered to have an immune system very similar to that of humans, as compared with mice, rats, and guinea pigs. Although primate allergic models to several pollen allergens have been developed, no model of house dust mite allergy has been reported. In this study, we attempted to induce type I allergy to mite allergens in rhesus monkeys. Methods: Six rhesus monkeys were immunized subcutaneously with crude mite extract adsorbed on aluminum hydroxide for 4 months. Then 5 monkeys positive for IgE production to mite extract were further immunized subcutaneously and conjunctivally with recombinant Der f 2 (rDer f 2). The status of sensitization to mite extract and rDer f 2 in monkeys was examined before and after the immunization. Plasma antigen-specific IgE and IgG levels, cutaneous reaction, and histamine release from peripheral blood leukocytes were measured. After conjunctival immunization, immediate conjunctivitis and leukocyte influx into conjunctiva after rDer f 2 challenge were examined. Results: After immunization with crude mite extract, 5 of 6 sensitized monkeys showed IgE response to the mite, and 4 out of 5 rDer f 2-sensitized monkeys exhibited IgE production to rDer f 2. Three monkeys sensitized with rDer f 2 showed immediate conjunctivitis and conjunctival eosinophilia after applying rDer f 2 to their eyes. Sensitized animals also showed IgG response to mite antigens. Conclusion: Four rhesus monkeys were positive for IgE production and allergic reactions to both mite extract and rDer f 2. These monkeys could represent a useful model for studying the development and regulation of house dust mite allergy.

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Section: Discussionmentioning

confidence: 99%

Experimental Monkey Model Sensitized with Mite Antigen

Yasue

Nakamura

Yokota

et al. 1998

Int Arch Allergy Immunol

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“…IgE antibodies, mast cells, basophils, eosinophils, neutrophils, macro phages and mucous cells, appear in asthmatic disor ders and their release of mediators seems to result in clinically manifested allergic inflammation and hy perreactivity [1]. The relative contribution of the var ious cells and their mediators in the disease has not been settled, however.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of the Bronchial Reactivity in Immunized Rats by Neonatal Treatment with Capsaicin

Ahlstedt¹,

Alving²,

Hesselmar

et al. 1986

Int Arch Allergy Immunol

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Rats were injected with capsaicin at 1–2 days of age to abolish the content of substance P (SP) in nerve terminals. At 6 weeks of age the capsaicin-treated and control rats were sensitized daily for 1 or 2 subsequent weeks Monday through Friday with ovalbumin (OA). The OA was given without adjuvant as 300 ng subcutaneous (s.c.) injections in the neck region or as 1% aerosol for 30 min. The capsaicin-treated animals which were sensitized s.c. for 2 weeks reacted moderately with increased transpulmonary pressure (TPP) to airway challenge with OA, and strongly to intravenous (i.v.) challenge with OA or serotonin. The capsaicin-untreated animals, which were sensitized with OA, reacted weakly to the challenge. In the animals sensitized with aerosolized OA, slightly lower reactivity was seen compared with those sensitized s.c. Untreated and unsensitized control rats reacted only to serotonin challenge. No animal had any detectable serum or bronchial IgE antibodies. Aerosol-sensitized animals had IgG antibodies in both serum and bronchial lavage. Histologically, the animals treated with capsaicin in contrast to the untreated controls demonstrated a pronounced increase of lymphoid tissue around their bronchi. Their mast cell numbers were increased around vessels and in the pleura and their mucous cell numbers were increased in the epithelium of the bronchi and bronchioli. The sensitization did not add much to this histological picture.

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“…In rodents it is possible to raise a humoral immune response restricted to distinct immunoglobulin classes by means of different adjuvants. Extracts of Ascaris, Nippostrongylus brasiliensis or Bordetella pertussis and lipopolysaccharide, alum or aluminiumhydroxide will potentiate an IgE-response, while Freund's complete adjuvant will principally induce IgG and suppress the IgE-response (5,105). In humans, IgE is similarly potentiated by extracts of bacteria and parasites mentioned above (5), whereas alum-and Al(OH)3-adsorbed allergen in two controlled immunotherapy studies have demonstrated clinical effects comparable to aqueous extracts (66,79).…”

Section: Mixture Emulgation or Adsorptionmentioning

confidence: 99%

“…Extracts of Ascaris, Nippostrongylus brasiliensis or Bordetella pertussis and lipopolysaccharide, alum or aluminiumhydroxide will potentiate an IgE-response, while Freund's complete adjuvant will principally induce IgG and suppress the IgE-response (5,105). In humans, IgE is similarly potentiated by extracts of bacteria and parasites mentioned above (5), whereas alum-and Al(OH)3-adsorbed allergen in two controlled immunotherapy studies have demonstrated clinical effects comparable to aqueous extracts (66,79). Compared with aque-ous allergen extract no higher levels of specific IgE were observed (66), w^hereas the levels of specific IgG were slightly increased (66) or unchanged (79).…”

Section: Mixture Emulgation or Adsorptionmentioning

confidence: 99%