2012
DOI: 10.1016/j.neuron.2012.03.027
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Relevance of Exocytotic Glutamate Release from Retinal Glia

Abstract: Glial cells release molecules that influence brain development, function, and disease. Calcium-dependent exocytosis has been proposed as potential release mechanism in astroglia, but the physiological relevance of "gliotransmission" in vivo remains controversial. We focused on the impact of glial exocytosis on sensory transduction in the retina. To this end, we generated transgenic mice to block exocytosis by Cre recombinase-dependent expression of the clostridial botulinum neurotoxin serotype B light chain, w… Show more

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Cited by 72 publications
(83 citation statements)
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“…Immunohistochemical staining of retinal sections and whole‐mounts from transgenic mice revealed expression of EGFP in Müller cells and astrocytes, but not in neurons or microglial cells (Figure 1a,b). We investigated whether dnSNARE expression impacted the calcium‐induced release of the gliotransmitters glutamate and ATP in single acutely isolated Müller cells using enzymatic assays (Slezak et al, 2012). UV photolysis‐induced calcium transients evoked the release of glutamate from Müller cells isolated from wildtype mice after loading with the photolabile chelator NP‐EGTA.…”
Section: Resultsmentioning
confidence: 99%
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“…Immunohistochemical staining of retinal sections and whole‐mounts from transgenic mice revealed expression of EGFP in Müller cells and astrocytes, but not in neurons or microglial cells (Figure 1a,b). We investigated whether dnSNARE expression impacted the calcium‐induced release of the gliotransmitters glutamate and ATP in single acutely isolated Müller cells using enzymatic assays (Slezak et al, 2012). UV photolysis‐induced calcium transients evoked the release of glutamate from Müller cells isolated from wildtype mice after loading with the photolabile chelator NP‐EGTA.…”
Section: Resultsmentioning
confidence: 99%
“…Transmitter release from acutely isolated Müller cells was measured using a fluorimetric enzyme assay as described (Slezak et al, 2012). Briefly, retinae were treated with papain (0.2 mg/ml; Roche Molecular Biochemicals) for 30 min at 37°C in the dark in Ca 2+ ‐ and Mg 2+ ‐free extracellular solution (140 mM NaCl, 3 mM KCl, 10 mM HEPES, 11 mM glucose, pH 7.4).…”
Section: Methodsmentioning
confidence: 99%
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