Relevance of Hepatocyte Growth Factor and Fibroblast Growth Factor on Mouse Preimplantation Embryo Development

Bahadori, Mohammad Hadi; Azarnia, Mahnaz; Ghasemian, Fatemeh; Ghasemi, Fahimeh; Ghadarjani, Shervin; Khojasteh, Narges; Moghaddam, M Ahmadi Jalali

doi:10.1016/s1001-7844(10)60001-6

Cited by 2 publications

(2 citation statements)

References 34 publications

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“…According to a previous study, a variety of growth factors were found in human ASC-CM including fibroblast, transforming, vascular endothelial, epidermal, insulin-like, granulocyte-macrophage colony-stimulating, and platelet-derived growth factors [19,33]. These are representative factors with supportive effects on preimplantation embryo development, which have been demonstrated for decades, in addition to an increase in blastocyst formation rate, cell number, expansion, hatching, protein synthesis, and decrease in apoptosis [7,[34][35][36][37][38]. Although the effectiveness of these factors present in ASC-CM has been proven, the treatment concentration should be determined in order to develop an optimal IVC medium.…”

Section: Discussionmentioning

confidence: 96%

Anti-Oxidative Effects of Human Adipose Stem Cell Conditioned Medium with Different Basal Medium during Mouse Embryo In Vitro Culture

Kim

et al. 2020

Animals

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The quality of embryos produced by assisted reproductive techniques should be advanced by the improvement of in vitro culture conditions for successful implantation and pregnancy maintenance. We investigated the anti-oxidative effect of human adipose stem cell (ASC) conditioned medium with its optimal basal medium, Dulbecco′s modified Eagle′s medium (DMEM-CM), or keratinocyte serum-free medium (KSFM-CM) as supplements during in vitro culture (IVC) of in vitro fertilized mouse embryo. At first, preimplantation embryo development was evaluated in KSFM-CM and DMEM-CM supplemented cultures at various concentrations. The blastocyst (BL) and hatched BL formation rates were significantly increased in 5% DMEM-CM, while no difference was observed from KSFM-CM. Next, comparing the efficacy of KSFM-CM and DMEM-CM at the same concentration, DMEM-CM enhanced the developmental rate of 16 cells, morula, BL, and hatched BL. The expression level of reactive oxygen species decreased and that of glutathione increased in BL cultured with DMEM-CM, which confirms its anti-oxidative effect. Furthermore, apoptosis in BL cultured with DMEM-CM was reduced compared with that in KSFM-CM. This study demonstrated that the comparative effect of human ASC-CM made of two different basal media during mouse embryo IVC and anti-oxidative effect of 5% DMEM-CM was optimal to improve preimplantation embryo development.

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Section: Discussionmentioning

confidence: 96%

Anti-Oxidative Effects of Human Adipose Stem Cell Conditioned Medium with Different Basal Medium during Mouse Embryo In Vitro Culture

Kim

et al. 2020

Animals

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“…Unfortunately, the suitable conditions for IVM of oocytes are still minimal due to the entire essential factors affecting IVM efficacy (7,8). In this regard, α-Linolenic acid (ALA) belongs to the group of omega-3, which can affect oocyte's growth and quality, and play an important role in the regulation of the meiotic stoppage at the stage of germinal vesicle (GV) (7).…”

Section: Introductionmentioning

confidence: 99%

In Vitro Oocyte Maturation Rate of Mice with Polycystic Ovarian Syndrome in the Presence of α-Linolenic Acid Antioxidant

Moradi

Ghasemian

Mashayekhi

2020

Zahedan J Res Med Sci

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Background: One of the most common endocrine and metabolic disorders is Polycystic ovary syndrome (PCOS), which has been reported in about 10% of women during the reproductive age. Objectives: This study was designed to investigate the efficiency of α-Linolenic acid (ALA) on in vitro maturation (IVM) and the quality of mouse oocytes with PCOS. Methods: Female NMRI mice (30 - 35 day-old) were developed by the injection of 4 mg estradiol valerate dissolved in 0.2 mg sesame oil for 60 consecutive days. In the following, the PCOS ovaries were dissected and oocytes were cultured in the maturation medium supplemented with different dosages of α-linolenic acid (0, 50, 100 µM). The presence of the first polar body was considered the sign of the nuclear maturation of the oocyte. The expression of mitochondrial transcription factor A (TFAM) gene in mature oocytes was investigated by Quantitative Real-time PCR. Results: The in vitro maturation and TFAM gene expression rates of PCOS oocytes in the medium treated with 50 µM of ALA (84 ± 7.9 and 0.46 ± 0.09, respectively) were significantly higher than the control group (P < 0.05). Conclusions: The ALA could improve the IVM rate and quality of PCOS oocytes by higher expression of TFAM gene.

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Relevance of Hepatocyte Growth Factor and Fibroblast Growth Factor on Mouse Preimplantation Embryo Development

Cited by 2 publications

References 34 publications

Anti-Oxidative Effects of Human Adipose Stem Cell Conditioned Medium with Different Basal Medium during Mouse Embryo In Vitro Culture

Anti-Oxidative Effects of Human Adipose Stem Cell Conditioned Medium with Different Basal Medium during Mouse Embryo In Vitro Culture

In Vitro Oocyte Maturation Rate of Mice with Polycystic Ovarian Syndrome in the Presence of α-Linolenic Acid Antioxidant

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