Relevance of Inflammatory Cytokine mRNA Expression of Tumour Necrosis Factor- Alpha (TNF α), Interleukin 17A (IL 17A) and Interleukin 6 (IL 6) in Indian Patients with Psoriasis

Abstract

Background: Psoriasis, a chronic, immune-mediated skin disorder, has systemic manifestations as well as an ample negative impact on the quality of life (QOL) of the patient. An abnormal proliferation of keratinocyte and dermal infiltration by immune cells is a characteristic feature. It involves components of both innate and adaptive immunity, and the interaction of T cells with macrophages. Keratinocytes and dendritic cells are mediated by the secreted cytokines. This study was taken up to look into changes at the molecular level that occur during the expression of three cytokines namely tumour necrosis factor–alpha (TNFα), interleukin 17A (IL-17A) and interleukin 6 (IL-6) in Indian patients with psoriasis. Methods: A case-control study was conducted with samples from 15 psoriasis vulgaris patients and 10 healthy control subjects. Clinical parameters were recorded. Blood samples were analysed for peripheral blood messenger ribonucleic acid (mRNA) expression of TNFα, IL-17A and IL-6 using real-time polymerase chain reaction (RT-PCR). Results: The mRNA expression of TNFα, IL-17A and IL-6 in psoriasis patients were increased as compared to that in normal subjects. Conclusions: The elevated levels of Interleukins indicates a systemic inflammatory process that is akin to the cutaneous inflammation. This study indicates that the targeted therapies against these cytokines are likely to be beneficial in Indian psoriasis patients.

Assessment of the Potential Role of Selected Single Nucleotide Polymorphisms (SNPs) of Genes Related to the Functioning of Regulatory T Cells in the Pathogenesis of Psoriasis

“…Our research also indicated a potential role of rs3093662 ( TNF ) in PsO etiology as we noticed that G allele carriers had a 2.175 times higher risk of developing PsO ( p = 2.40 × 10 −6 ). Similarly to other studies, we observed a significant increase in mRNA TNF in PsO [ 26 , 27 ]. Therefore, it seems likely that the involvement of genetic factors in TNF function may indirectly contribute to the Treg-mediated response in PsO, but this requires further research.…”

Serum concentrations of 25-OH vitamin D and the pro-inflammatory interleukins IL-17, IL-23, and IL-18 in patients with plaque psoriasis

“…The variations of cytokine levels across studies may be due to different study designs, different genetic backgrounds, or may reflect the influence of concomitant diseases. A recent study of psoriasis patients without any associated comorbidities using highly sensitive methods of detecting cytokine mRNA provides evidence of increased mRNA expression of IL-17A in blood samples of patients compared to that in normal subjects 57 thus emphasizing the major role of IL-17 in psoriasis.…”