Relevance of miR-223 as Potential Diagnostic and Prognostic Markers in Cancer

Aziz, Faisal; Chakraborty, Abhijit; Khan, Imran; Monts, Josh

doi:10.3390/biology11020249

Cited by 18 publications

(18 citation statements)

References 104 publications

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“…In addition to functioning in the hematopoietic system, miR-223 is in close relationship with multiple malignancies, given that miR-223 targets (e.g., IGF-1 receptor and stathmin 1) have been disclosed to impact the hallmarks of cancer, involving proliferation, invasion, metastasis, etc. ( 185 ). In vitro and in vivo studies have substantiated that the upregulated expression of miR-223 dampens therapeutic sensitivity in several cancer types, such as T-ALL, GC, NSCLC, HCC, and CRC (seen in Table 1 ).…”

Section: Perspectives: Potential Therapeutic Strategiesmentioning

confidence: 99%

FBXW7 and the Hallmarks of Cancer: Underlying Mechanisms and Prospective Strategies

et al. 2022

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FBXW7, a member of the F-box protein family within the ubiquitin–proteasome system, performs an indispensable role in orchestrating cellular processes through ubiquitination and degradation of its substrates, such as c-MYC, mTOR, MCL-1, Notch, and cyclin E. Mainly functioning as a tumor suppressor, inactivation of FBXW7 induces the aberrations of its downstream pathway, resulting in the occurrence of diseases especially tumorigenesis. Here, we decipher the relationship between FBXW7 and the hallmarks of cancer and discuss the underlying mechanisms. Considering the interplay of cancer hallmarks, we propose several prospective strategies for circumventing the deficits of therapeutic resistance and complete cure of cancer patients.

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Section: Perspectives: Potential Therapeutic Strategiesmentioning

confidence: 99%

FBXW7 and the Hallmarks of Cancer: Underlying Mechanisms and Prospective Strategies

et al. 2022

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“…The first microRNA (miRNA) was the heterogeneous gene lin-4 discovered from Caenorhabditis elegans in 1993 [ 62 ]. MiRNAs originate from DNA introns or exons which are transcribed by RNA polymerase II and III into a 1 kb long primary-miRNA with a hairpin-like structure.…”

Section: Microrna and Ibdmentioning

confidence: 99%

Updated immunomodulatory roles of gut flora and microRNAs in inflammatory bowel diseases

2022

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Inflammatory bowel disease is a heterogeneous intestinal inflammatory disorder, including ulcerative colitis (UC) and Crohn's disease (CD). Existing studies have shown that the pathogenesis of IBD is closely related to the host's genetic susceptibility, intestinal flora disturbance and mucosal immune abnormalities, etc. It is generally believed that there are complicated interactions between host immunity and intestinal microflora/microRNAs during the occurrence and progression of IBD. Intestinal flora is mainly composed of bacteria, fungi, viruses and helminths. These commensals are highly implicated in the maintenance of intestinal microenvironment homeostasis alone or in combination. MiRNA is an endogenous non-coding small RNA with a length of 20 to 22 nucleotides, which can perform a variety of biological functions by silencing or activating target genes through complementary pairing bonds. A large quantity of miRNAs are involved in intestinal inflammation, mucosal barrier integrity, autophagy, vesicle transportation and other small RNA alterations in IBD circumstance. In this review, the immunomodulatory roles of gut flora and microRNAs are updated in the occurrence and progression of IBD. Meanwhile, the gut flora and microRNA targeted therapeutic strategies as well as other immunomodulatory approaches including TNF-α monoclonal antibodies are also emphasized in the treatment of IBD.

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“…miR-223 has an evolutionary conserved binding site at the 3′UTR of the NLRP3 transcript [ 22 ]. Although miR-223 has been implicated in reducing NLRP3 expression and IL-1β secretion in a variety of inflammatory diseases [ 23 ], data on its effects in CRC are inconsistent [ 24 , 25 , 26 ]. Therefore, in this study, we investigated the effect of the P2X7 inhibitor, gli, as a blocking agent for assembling NLRP3 and the effect of miR-223 as a posttranslational inhibitor of NLRP3 in HCT-116 and HCT-15 CRC cell lines under NLRP3 activation.…”

Section: Introductionmentioning

confidence: 99%

Implications of NLRP3 Suppression Using Glibenclamide and miR-223 against Colorectal Cancer

Hamza,

Garanina,

Shkair

et al. 2024

Pharmaceuticals

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The NLR family pyrin domain containing 3 (NLRP3) promotes the growth of colorectal cancer (CRC). However, the therapeutic effect of NLRP3 inhibition on CRC cell progression is controversial. This study comparatively investigated the therapeutic effect of a pharmacological NLRP3 inhibitor, glibenclamide (gli), and the post-translational suppression of NLRP3 by miR-223 on CRC cell progression in HCT-116 and HCT-15 cells. LPS and ATP were used to activate Gli-treated and LSB-hsa-miR-223-3p (WTmiR-223)-expressing HCT-116 cells. NLRP3.AB.pCCL.sin.cPPT.U6.miR-223-Decoy.hPGK.GFP.WPRE plasmid (DmiR-223) was the negative control for miR-223 expression. NLRP3, gasdermin D, and BAX expressions were analyzed using western blotting. Real-time PCR detected the RNA expression of autophagy-related genes ATG5, BECN1, and miR-223 in non-transfected cells. ELISA analyzed IL-1β and IL-18 in the medium. MTS-1, annexin V, wound-healing, and sphere-invasion assays were used to assess cell viability and progression. A multiplex cytokine assay detected proinflammatory cytokine secretion. LPS–ATP-activated NLRP3 produced gasdermin D cleavage, released IL-1b and IL-18, and activated cell migration and sphere invasion. In contrast, reduced cell growth, miR-223 expression, IFN-γ, CXCL10, and LIF secretion were found in cells after inflammasome activation. Both gli and WTmiR-223 induced autophagy genes ATG5 and BECN1 and reduced the NLRP3 activation and its downstream proteins. However, while gli had a limited effect on the production of IFN-γ, CXCL10, and LIF, WTmiR-223 increased the release of those cytokines. In addition, gli did not suppress cell growth, while WTmiR-223 promoted apoptosis. Notably, neither gli nor WTmiR-223 effectively prevented sphere invasion. These data suggest that, while WTmiR-223 could have a better anticancer effect in CRC compared to gli, the sole usage of miR-223-mediated NLRP3 suppression may not be sufficient to prevent CRC metastasis.

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Relevance of miR-223 as Potential Diagnostic and Prognostic Markers in Cancer

Cited by 18 publications

References 104 publications

FBXW7 and the Hallmarks of Cancer: Underlying Mechanisms and Prospective Strategies

FBXW7 and the Hallmarks of Cancer: Underlying Mechanisms and Prospective Strategies

Updated immunomodulatory roles of gut flora and microRNAs in inflammatory bowel diseases

Implications of NLRP3 Suppression Using Glibenclamide and miR-223 against Colorectal Cancer

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