Relevant factors affecting microbial surface decontamination by pulsed light

Levy, Caroline; Aubert, X; Lacour, Bernard; Carlin, Frédéric

doi:10.1016/j.ijfoodmicro.2011.08.022

Cited by 95 publications

(68 citation statements)

References 37 publications

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“…These values are consistent with previous results obtained for hepatitis A virus and poliovirus type 1 treated with pulsed light (13,14) and for MNV-1 treated with continuous UV light (34,35). As observed in the case of bacteria and fungi (36), inactivation of MNV-1 appeared equivalent whether the light was continuous or pulsed but was faster with pulsed light (232 s [34] versus 1.5 s).…”

Section: CMsupporting

confidence: 91%

Efficacy and Mechanisms of Murine Norovirus Inhibition by Pulsed-Light Technology

Vimont

Fliss

Jean

2015

Appl Environ Microbiol

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Pulsed light is a nonthermal processing technology recognized by the FDA for killing microorganisms on food surfaces, with cumulative fluences up to 12 J cm ؊2 . In this study, we investigated its efficacy for inactivating murine norovirus 1 (MNV-1) as a human norovirus surrogate in phosphate-buffered saline, hard water, mineral water, turbid water, and sewage treatment effluent and on food contact surfaces, including high-density polyethylene, polyvinyl chloride, and stainless steel, free or in an alginate matrix. The pulsed-light device emitted a broadband spectrum (200 to 1,000 nm) at a fluence of 0.67 J cm ؊2 per pulse, with 2% UV at 8 cm beneath the lamp. Reductions in viral infectivity exceeded 3 log 10 in less than 3 s (5 pulses; 3.45 J cm ؊2 ) in clear suspensions and on clean surfaces, even in the presence of alginate, and in 6 s (11 pulses; 7.60 J cm ؊2 ) on fouled surfaces except for stainless steel (2.6 log 10 ). The presence of protein or bentonite interfered with viral inactivation. Analysis of the morphology, the viral proteins, and the RNA integrity of treated MNV-1 allowed us to elucidate the mechanisms involved in the antiviral activity of pulsed light. Pulsed light appeared to disrupt MNV-1 structure and degrade viral protein and RNA. The results suggest that pulsed-light technology could provide an effective alternative means of inactivating noroviruses in wastewaters, in clear beverages, in drinking water, or on food-handling surfaces in the presence or absence of biofilms.H uman noroviruses are responsible for one-fifth of all cases of acute gastroenteritis worldwide (1). They spread directly via person-to-person contact (fecal-oral and vomitus-oral) or indirectly through food, water, and the environment (2). Natural biofilms in sewage treatment effluent could be responsible for many persistent waterborne outbreaks, since such biofilms have been found to harbor noroviruses (3, 4). Infection spreads quickly and widely, affecting individuals of all ages, especially where many people gather or live in close proximity, such as in long-term care residences, retirement homes, cruise ships, and the like. Infectious doses as low as 2,800 particles, the high viral loads in feces and vomit (up to 10 9 genomic copies per gram), persistence in the environment, prolonged duration of viral shedding even after symptoms have resolved, and inadequate long-term immunity all contribute to the high incidence of norovirus illness (5). Although the illness is usually mild and self-limiting, the large number of cases per year represents a considerable loss in productivity and constitutes a substantial burden on society (2, 6).Among the physical disinfection processes, pulsed-light treatment appears well suited for the disinfection of food contact surfaces in industrial and health care settings and for decontaminating water and beverages. It has been proposed as a means of nonthermal pasteurization for food preservation and for decontaminating air and packaging materials (7). It has proven effective for inactivati...

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Section: CMsupporting

confidence: 91%

Efficacy and Mechanisms of Murine Norovirus Inhibition by Pulsed-Light Technology

Vimont

Fliss

Jean

2015

Appl Environ Microbiol

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“…Complete transformation of bacterial spores into vegetative cells could be responsible for this enhanced sensibility to PL treatment, as previously mentioned for heat treatments. Correspondingly, some previous studies have shown that spores would be considerably more resistant to PL treatment than vegetative population (Dunn et al 1989;Levy et al 2012). The PL sensitivity of B. subtilis cells, however, did not significantly increase after 3 h (Fig.…”

Section: Effect Of Pl Treatment On Culturability Of B Subtilis Cellsmentioning

confidence: 51%

“…This novel technology consists of a successive repetition of short duration and high power flashes of broadband emission light (190-1000 nm) with approximately 40 % of the emitted light corresponding to the UV region (Wekhof 2000). PL has been shown to be effective in inactivating a wide broad of microorganisms, both vegetative cells (Gómez-López et al 2005;Lasagabaster and Martínez de Marañón 2012) and spores (Dunn et al 1989;Levy et al 2012). However, as germination is a key step for spores to exert their deleterious effect, an important factor to be considered for their application in food industry which has not been elucidated yet is the effect of PL on this metabolic process.…”

Section: Introductionmentioning

confidence: 99%

Effect of Pulsed Light Treatment on the Germination of Bacillus subtilis Spores

Artíguez

Marañón

2014

Food Bioprocess Technol

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The loss of cell culturability (spore counts), spore germination and subsequent vegetative growth of Bacillus subtilis were evaluated after pulsed light (PL) treatment. The effect of PL on those mechanisms was dependent on the total fluence applied, decreasing the spore counts when increasing total fluence (0.3-12 J/cm 2 ). Neither spore germination nor vegetative growth was affected after exposure to low total fluence (0.3 J/cm 2 ). A fluence between 1 and 2 J/cm 2 did not change the rate of germination, but significantly delayed bacterial growth. The lag period before growth increased with the total fluence. For higher total fluence (5.5 J/cm 2 ), the rate of spore germination was considerably reduced. When B. subtilis spores were subjected to 12 J/cm 2 , no cultivable cells were detected (the maximum detectable level of cell inactivation was reached), being both spore germination and vegetative growth prevented for at least 48 h. For PL treatments in which germination occurred, a temporary increase in the resistance of B. subtilis cells to PL was found in the early phase of germination except for the lowest fluence (0.5 J/cm 2 ). After this period, cells became more sensitive, having similar resistance to PL during exponential and stationary phase of growth. PL sensitized B. subtilis cells (spore or vegetative forms) to posterior thermal treatments, demonstrating a synergistic effect of heat and PL which points out the potential application of such combination of treatments for microbial inactivation.

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“…The time to obtain 4elog reduction (t 4D ) was calculated from the model parameters (d and b) by employing Eq. (2) (Levy, Aubert, Lacour, & Carlin, 2012).…”

Section: Modeling Of Inactivation Datamentioning

confidence: 99%

Evaluation of bioactivity of pomegranate fruit extract against Alicyclobacillus acidoterrestris DSM 3922 vegetative cells and spores in apple juice

Molva

Baysal

2015

LWT - Food Science and Technology

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5 log CFU/mL) in apple juice (pH 3.82, Brix 11.3) during storage at 37 C. After 240 h, the cell counts were reduced from the initial log count (CFU/mL) by 2. 84, 3.26, 3.32, 3.46 and 3.56 in the apple juice with PE at the concentrations of 2.5, 5, 10, 20 and 40 mg/mL, respectively. On the other hand, counts of the control reached 7.36 log CFU/mL after 24 h. The Weibull model satisfactorily described the survival curves of cell inactivation kinetics (R 2 > 0.983). While the growth of all spores obtained from different sporulation media (potato dextrose agar, malt extract agar, Bacillus acidoterrestris agar, and Bacillus acidocaldarius agar) was inhibited in the apple juice with PE (2.5e40 mg/mL), the control spores increased by 1.9e2.2 log CFU/mL after 336 h. Based on the scanning electron microscopy (SEM) imaging, vegetative cells indicated substantial damage and spore germination was inhibited in the apple juice with PE. The results showed that PE can have possible uses as a natural antimicrobial to control the growth of A. acidoterrestris vegetative cells and spore germination in the apple juice.

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Relevant factors affecting microbial surface decontamination by pulsed light

Cited by 95 publications

References 37 publications

Efficacy and Mechanisms of Murine Norovirus Inhibition by Pulsed-Light Technology

Efficacy and Mechanisms of Murine Norovirus Inhibition by Pulsed-Light Technology

Effect of Pulsed Light Treatment on the Germination of Bacillus subtilis Spores

Evaluation of bioactivity of pomegranate fruit extract against Alicyclobacillus acidoterrestris DSM 3922 vegetative cells and spores in apple juice

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