Relevant insight of surface characterization techniques to study covalent grafting of a biopolymer to titanium implant and its acidic resistance

D’Almeida, Mélanie; Amalric, Julien; Brunon, Céline; Grosgogeat, Brigitte; Toury, Bérangère

doi:10.1016/j.apsusc.2014.11.185

Cited by 15 publications

(9 citation statements)

References 56 publications

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“…The deconvoluted peaks of C 1s gave four components: components at 289 eV (2.0 ± 0.1%), at 287.6 eV (15.9 ± 1.5%), at 286.2 eV (51.5 ± 2.8%) and at 284.8 eV (30.7± 4.0%) which were assigned to N- C = O, O- C -O/ C = O, C -O/ C -N and C -C/ C -H respectively [ 39 , 40 ]. These results confirm the success of chitosan grafting as previously noticed [ 27 , 28 ].…”

Section: Resultssupporting

confidence: 92%

“…Measurements were performed using a ToF-SIMS 5 instrument (ION-TOF GmbH, Germany) following analysis and calibration conditions reported in our previous study [ 27 ]. A pulsed primary ion source of Bi 3 + was operated at 25 KeV.…”

Section: Methodsmentioning

confidence: 99%

“…ToF-SIMS 5 instrument (ION-TOF GmbH, Germany) was used with a pulsed primary ion source of Bi 1 + operated at 25 KeV for analysis, the same operating conditions were selected as used in a previous study [ 27 ]. Scanning area of secondary ions was 100 μm × 100 μm in the center of a larger sputtered area, in order to minimize edge effects and redeposition linked to the formation of the crater.…”

Section: Methodsmentioning

confidence: 99%

“…In order to bond chitosan to a titanium support, several methods are considered in the literature; such as the dopamine glutaraldehyde method [ 21 ], the electrodeposition of chitosan on substrates [ 22 ], or the use of catechol functional groups [ 23 ]. Another strategy consists of using an organosilane as a coupling agent; such as 3-AminoPropylTriEthoxySilane (APTES) associated with glutaraldehyde or succinic anhydride [ 24 – 27 ] or TriEthoxySilylPropylSuccinic Anhydride (TESPSA) [ 28 ]. Which is often selected as an intermediate between biomolecules and titanium due to its inherent antibacterial and biological properties [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

“…Recent in vitro studies focused on the surface characterization, especially on the interface between an animal-based chitosan coating and metal support using both bulk and outermost surface characterization techniques [ 27 ]. Our group demonstrated the cytocompatiblity of grafted animal chitosan using TESPSA as coupling agent in the presence of fibroblasts [ 28 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

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Chitosan coating as an antibacterial surface for biomedical applications

D’Almeida

Attik

Amalric³

et al. 2017

PLoS ONE

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Background and objectivesA current public health issue is preventing post-surgical complications by designing antibacterial implants. To achieve this goal, in this study we evaluated the antibacterial activity of an animal-free chitosan grafted onto a titanium alloy.MethodsAnimal-free chitosan binding on the substrate was performed by covalent link via a two-step process using TriEthoxySilylPropyl Succinic Anhydride (TESPSA) as the coupling agent. All grafting steps were studied and validated by means of X-ray Photoelectron Spectroscopy (XPS), Time-of-Flight secondary ion mass spectrometry (ToF-SIMS) analyses and Dynamic-mode Secondary Ion Mass Spectrometry (DSIMS). The antibacterial activity against Escherichia coli and Staphylococcus aureus strains of the developed coating was assessed using the number of colony forming units (CFU).ResultsXPS showed a significant increase in the C and N atomic percentages assigned to the presence of chitosan. A thick layer of polymer deposit was detected by ToF-SIMS and the results obtained by DSIMS measurements are in agreement with ToF-SIMS and XPS analyses and confirms that the coating synthesis was a success. The developed coating was active against both gram negative and gram positive tested bacteria.ConclusionThe success of the chitosan immobilization was proven using the surface characterization techniques applied in this study. The coating was found to be effective against Escherichia coli and Staphylococcus aureus strains.

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Section: Resultssupporting

confidence: 92%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Chitosan coating as an antibacterial surface for biomedical applications

D’Almeida

Attik

Amalric³

et al. 2017

PLoS ONE

Self Cite

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Experimental and molecular dynamics simulation study on antifouling performance of antimicrobial peptide‐modified aluminum alloy surfaces

Liu,

Lou,

Bai

et al. 2024

Surface & Interface Analysis

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Marine biofouling poses a major challenge to ship navigation and hinders the development of the shipping industry. Urgent action is required to tackle this problem through the implementation of innovative strategies. Antimicrobial peptides have garnered considerable attention due to their outstanding effectiveness, wide range of activity, and eco‐friendly characteristics. This study involved grafting the antibacterial peptide andricin 01 (AIGHCLGATL) onto the surface of an aluminum alloy, thereby creating a modified surface with antibacterial properties. In summary, amino groups were introduced onto the surface of aluminum alloys through the silanization process using (3‐aminopropyl) triethoxysilane (APTES), and then the peptides were covalently immobilized on the treated surface using glutaraldehyde as a cross‐linking agent. The successful modification of the peptide was confirmed by Fourier transform‐infrared spectroscopy (FT‐IR) and X‐ray photoelectron spectroscopy (XPS) analysis. The antimicrobial peptide‐modified aluminum alloy surfaces exhibited significant bactericidal activity, killing 75.3% of Bacillus sp. and 85.5% of Escherichia coli, while achieving antifouling efficiencies of 88.6% and 90.7% against Bacillus sp. and E. coli, respectively. Furthermore, molecular dynamics simulations showed that the inserted of the peptides into the phospholipid membrane caused a change in the local membrane curvature, which eventually led to membrane rupture. These results provide valuable information for the application of antimicrobial peptides in the field of antifouling and the elucidation of antifouling mechanisms.

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Chitosan and Graphene Oxide Nanocomposites as Coatings for Controlled-Release Fertilizer

Gao

Tong

et al. 2019

Water Air Soil Pollut

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Relevant insight of surface characterization techniques to study covalent grafting of a biopolymer to titanium implant and its acidic resistance

Cited by 15 publications

References 56 publications

Chitosan coating as an antibacterial surface for biomedical applications

Chitosan coating as an antibacterial surface for biomedical applications

Experimental and molecular dynamics simulation study on antifouling performance of antimicrobial peptide‐modified aluminum alloy surfaces

Chitosan and Graphene Oxide Nanocomposites as Coatings for Controlled-Release Fertilizer

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