In the process of machining, the operator’s incorrect operation using the control human-computer interaction interface may lead to a disastrous effect on the whole production chain such as task failure and system failure. The eight types of using errors about human-computer interaction interface system (misperception, memory lapse, carelessness, improper decision-making, improper operation, improper interface layout design, improper icon and text display design, and improper prompt feedback) are proposed based on the cognition from users. According to the eight types of using error, the human reliability and safety evaluation method is proposed. Considering the variability objective and uncertainty subjective factors in the human-machine interaction process, the human reliability evaluation method is proposed based on objective and subjective comprehensive weighting under ergonomic principles. Firstly, based on the investigation of relevant machine tool interface design, the comprehensive evaluation index system for man-machine reliability of complex interface design is established. Secondly, an objective and subjective comprehensive weight assignment method as the composition of Theilʼs entropy weight method combined with TOPSIS (Technique for Order Preference by Similarity to an Ideal Solution) and improved AHP (analytic hierarchy process) method combined with fuzzy mathematics is proposed. It can not only effectively avoid the impact of excessive subjective factors on empowerment but also fully excavate the real information in the survey data and improve the objectivity and accuracy of the weight data of evaluation indicators at all levels. Thirdly, taking the interface design of CNC machine tool equipment for example, the best human-computer interaction interface design schemes are chosen according to the human reliability evaluation method based on objective and subjective comprehensive method calculated by Entropy-TOPSIS-AHP. Finally, based on eye movement experiments, the use efficiency and accuracy of the human-computer interaction interface design scheme chosen by the assessment method are better than the other design schemes or original one to verify the assessment method validity.