The assessment of existing reinforced concrete structures is one of the major aspects for engineers and practitioners. In particular, existing infrastructures, as bridges and viaducts, are extensively exposed to environmental actions, materials aging, degradation, and variation of magnitude of traffic loads during their service life. Hence, the assessment of existing structural systems assuming the same criteria conceived for the design (i.e., partial factor method—EN 1990) can be too conservative and, sometimes, may lead to unnecessary and expensive structural interventions. In this context, fib Bulletin 80 defines the partial factor methods suitable for the assessment of existing reinforced concrete structures accounting for their residual service life, information from in situ and laboratory tests, measurements of variable actions and reduced target reliability levels according to both economical and human safety criteria. The methodologies proposed in fib Bulletin 80 have been applied to assess the safety of an existing prestressed reinforced concrete bridge built in 90s and located in Italy. The results are compared to the outcomes from the assessment performed according to EN1990 and, finally, limits and advantages of the methodologies proposed by fib Bulletin 80 are discussed.