Reliability of automated synovial fluid cell counting with Mindray BC‐6800 body fluid mode

Abstract

The use of BC-6800-BF for synovial fluid analysis enables rapid and accurate assessment, especially for total cell and polymorphonuclear counts. The use of BC-6800-BF may therefore allow the replacement of optical analysis, especially in samples pretreated with hyaluronidase, thus allowing its routine use for the screening of synovial specimens.

Lack of harmonization in high fluorescent cell automated counts with body fluids mode in ascitic, pleural, synovial, and cerebrospinal fluids

“…The final pre treatment of SF samples consisted in mixing 20 µL of the HY solution with 1 mL of fluid, followed by incubation at room temperature for 5 minutes. Cell counts were then simultaneously performed with XN‐BF, BC‐BF, and OM …”

“…Cell count was carried out in Nageotte or Burker counting chambers. AF, PF, and CSF samples were diluted (1:2 or 1:20 or 1:200) with Turk's solution (Carlo Erba, Milan, Italy), whilst SF samples were diluted (1:2 or 1:20 or 1:200) with Stromatol (Biolife, Masha Brunelli, Italy) . Optical counts were performed at ×400 magnification.…”

“…Cell counting and classification in body fluids (BFs; ascitic [AF], pleural [PF], synovial [SF], and cerebrospinal [CSF]) has become very popular in recent times due to enhanced availability of new commercial instrumentation . Carried out using suitable analyzers, automated cell count can now be regarded as a useful screening, since is adequately precise, may substantially reduce the turnaround time (TAT), is available 24 hours a day, and allows better standardization of the analytical process …”

“…Although all hematological analyzers (also referred to as “hemocytometers”) equipped with the BF‐mode can generate both quantitative and qualitative measures, these parameters differ among the various commercial analyzers, being mostly instrument‐dependent. Quantitative parameters may include total cell count (TC), leukocyte count (WBC) and, sometimes, differential cell count capable to discriminate between polymorphonuclear (PMN) and mononuclear (MN) leukocytes . The two hematological analyzers of Sysmex XN series (Sysmex, Kobe Japan) and the Mindray BC‐6800 (Mindray, Shenzhen, China) are equipped with a BF‐mode channel dedicated to cell count and classification, which is based on optical fluorescence reading, selective lysis of red blood cells (RBCs), along with specific hardware and fluidics.…”

“…The XN‐module in BF mode (XN‐BF) provides the following diagnostic parameters: TC (XN‐TC‐BF), WBC (XN‐WBC‐BF), PMN (XN‐PMN‐BF), MN (XN‐MN‐BF), and high fluorescent cells (XN‐HF‐BF) counts, as well as additional research parameters such as neutrophil (XN‐NE‐BF), lymphocyte (XN‐LY‐BF), monocyte (XN‐MO‐BF), and eosinophil (XN‐EO‐BF) counts . The BC‐6800 in BF mode (BC‐BF) generate TC (BC‐TC‐BF), WBC (BC‐WBC‐BF), PMN (BC‐PMN‐BF), MN (BC‐MN‐BF), and high fluorescent cells (BC‐HF‐BF) counts, along with other research parameters such as neutrophil (BC‐NE‐BF) and eosinophil (BC‐EO‐BF) counts . For both XN‐BF and BC‐BF modes, the number of cells classified as HF‐BF is significantly correlated with the presence of mesothelial and/or various neoplastic cells .…”