Reliability of follicle-stimulating hormone measurements in serum

Arslan, Alan A.; Zeleniuch‐Jacquotte, Anne; Lukanova, Annekatrin; Rinaldi, Sabina; Kaaks, Rudolf; Toniolo, Paolo

doi:10.1186/1477-7827-1-49

Cited by 19 publications

(7 citation statements)

References 24 publications

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“…The study results confirm the previous reports that a single measurement is sufficient to characterize the serum FSH level in postmenopausal women (18, 19) but not in premenopausal women (18) unless phase of menstrual cycle is taken into account. A similar conclusion can be made in regard to the reproducibility of LH in serum.…”

Section: Discussionsupporting

confidence: 90%

Reproducibility of Serum Pituitary Hormones in Women

Arslan

Gu²,

Zeleniuch‐Jacquotte

et al. 2008

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Endogenous pituitary hormones are commonly used in clinical and epidemiological studies and some of them are thought to influence risk of several diseases in women. In most studies, endogenous levels of pituitary hormones are usually assessed at a single point in time, assuming that this single measurement represents the long-term biomarker status of the individual. Such an assumption is rarely tested and may not always be valid. This study examined the reproducibility of the following pituitary hormones: adrenocorticotropic hormone (ACTH), growth hormone (GH), follicle-stimulating hormone (FSH), luteinizing hormone (LH), thyroid-stimulating hormone (TSH), and prolactin (PRL) measured using the Luminex xMap™ method in serum of healthy premenopausal and postmenopausal women. The study included 30 premenopausal women with three yearly samples and 35 postmenopausal women with two repeated yearly samples randomly selected from an existing prospective cohort. Analysis of intraclass correlation coefficients (ICCs) suggested higher reproducibility in postmenopausal women compared to premenopausal women for the following hormones: FSH (0.72 and 0.37, respectively), LH (0.83 and 0.44, respectively), and GH (0.60 and 0.35, respectively). The ICCs were relatively high and similar between postmenopausal and premenopausal women for ACTH (0.95 and 0.94, respectively), TSH (0.85 and 0.85, respectively), and PRL (0.72 and 0.69, respectively). This study found that serum concentrations of FSH, LH, and GH are stable in postmenopausal women, and that ACTH, TSH, and PRL are stable in both premenopausal and postmenopausal women suggesting that a single measurement may reliably categorize average levels over at least a 2-year period.

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Section: Discussionsupporting

confidence: 90%

Reproducibility of Serum Pituitary Hormones in Women

Arslan

Gu²,

Zeleniuch‐Jacquotte

et al. 2008

Cancer Epidemiology, Biomarkers &Amp; Prevention

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“…Because serum FSH levels provide insight into the functional capacity of the hypothalamus, pituitary, and ovary or testis, they are frequently used for clinical evaluation of infertility, and disorders of the hypothalamic-pituitary-gonadal axis [1]. Specifically, FSH levels can be used to evaluate the etiology of low sperm counts, amenorrhea, menstrual irregularities, pituitary disorders, precocious or delayed puberty, and ovarian/testicular dysfunction [2,3].…”

Section: Introductionmentioning

confidence: 99%

Inter-laboratory validation of the measurement of follicle stimulating hormone (FSH) after various lengths of frozen storage

Scriver

Baker

Young

et al. 2010

Reprod Biol Endocrinol

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BackgroundSerum follicle stimulating hormone (FSH) levels are used clinically to evaluate infertility, pituitary and gonadal disorders. With increased frequency of research collaborations across institutions, it is essential that inter-laboratory validation is addressed.MethodsAn inter-laboratory validation of three commercial FSH immunoassays was performed with human serum samples of varying frozen storage length (2 batches of 15 samples each) at -25 degree C. Percentage differences and Bland-Altman limits of agreement were calculated.ResultsThe inter- and intra-laboratory consistency of FSH values with the same assay manufacturer was much higher after shorter-term storage (frozen for less than 11 months, mean percentage degradation less than 4%) than after long-term storage (2-3 years, mean percentage degradation = 23%). Comparing assay results from different manufacturers, there was similar overall long term degradation as seen with the same manufacturer (-25%), however the degradation was greater when the original FSH was greater than 20 mIU/mL relative to less than 10 mIU/mL (p < 0.001 trend test).ConclusionThe findings suggest that degradation of serum samples stored between 11 months and 2-3 years at -25 degrees C can lead to unstable FSH measurements. Inter-laboratory variability due to frozen storage time and manufacturer differences in assay results should be accounted for when designing and implementing research or clinical quality control activities involving serum FSH at multiple study sites.

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“…For laboratory investigations, the anti-Mullerian hormone [AMH] was measured by the method described by Kumar et al [15] , FSH determined by the technique described in Arslan et al [16] , LH determined according to Pauerstein et al [17] , and testosterone hormone [LH] measurements [18] .…”

Section: Methodsmentioning

confidence: 99%

Effects of Different Methods of Laparoscopic Ovarian Drilling and its Outcome in Patients with Polycystic Ovary Syndrome

Hamoda

Megahed

Oun

2021

International Journal of Medical Arts

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Background: Polycystic ovary syndrome [PCOS] is an important and common etiology for anovulatory infertility. Clomiphene citrate [CC] is the first line of treatment. One fourth of females are resistant to CC. Laparoscopic ovarian drilling [LOD] has developed manage CC-resistant cases. However, no consensus was established on the ideal drilling method. Aim of the work: This study aimed to evaluate the efficacy of different LOD methods for anovulatory infertility in CC-resistant PCOS patients. Patients and Methods: The study participants [200] were assigned randomly to one of four equal groups. Group [I] treated by unilateral cutting LOD. Group [II] treated by unilateral electro-coagulative LOD. Group [III] treated by bilateral cutting LOD. Group [IV] treated by bilateral electro-coagulative LOD. All participants were submitted to history taking, clinical examination, laboratory investigation, antral follicular count [AFC], and transvaginal ultrasound. After LOD, follow up included regularity of menstrual cycle, ovulation, and hormonal assay [at six months after ovarian drilling]. Also, ovulation, pregnancy and miscarriage rates were documented. Results:The study groups showed no significant difference regarding patient characteristics [e.g., body mass index, infertility duration and menstrual cycle history], and hormonal profile before and after LOD. The spontaneous ovulation rate was 40%, 42.0%, 46.0% and 40.0% in groups I, II, III and IV, respectively. The pregnancy rate was 32.0%, 38.0%, 44.0% and 42.0% in groups I, II, III and IV, respectively.in all groups, hormonal profile and AFC showed significant improvement after LOD compared with corresponding pre-LOD values. However, levels of FSH are significantly nonsignificant. Conclusion:Different techniques of LOD are equally effective and safe [e.g., unilateral ovarian drilling is as effective and safe as bilateral drilling, regardless of the drilling method [coagulative or cutting] with comparable ovulation, pregnancy and miscarriage rates.

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Reliability of follicle-stimulating hormone measurements in serum

Cited by 19 publications

References 24 publications

Reproducibility of Serum Pituitary Hormones in Women

Reproducibility of Serum Pituitary Hormones in Women

Inter-laboratory validation of the measurement of follicle stimulating hormone (FSH) after various lengths of frozen storage

Effects of Different Methods of Laparoscopic Ovarian Drilling and its Outcome in Patients with Polycystic Ovary Syndrome

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