Reliability of Kirby-Bauer disk diffusion method for detecting meropenem resistance among non-fermenting gram-negative bacilli

Joseph, Noyal Mariya; Sistla, Sujatha; Dutta, Tarun Kumar; Badhe, Ashok Shankar; Rasitha, Desdemona; Parija, Subhash Chandra

doi:10.4103/0377-4929.85092

Cited by 22 publications

(18 citation statements)

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“…One of the standard assay methods for testing antimicrobial activity is the Kirby-Bauer method [24], also referred to as the disc diffusion method. A selective culture media were prepared in the antimicrobial assay container and subsequently streaked uniformly with the selected test microorganisms.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

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Qualitative Analysis of Peptides and Biological Activities of Allexis cauliflora (Violaceae) Leaves

Ditchou

Soh

Tchouboun

et al. 2020

JNPR

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Allexis cauliflora is a Cameroonian medicinal plant belonging to the violaceae family. It is used in traditional medicine to treat fever, syphilis and malaria. The aim of this study is to screen the peptides from the leaves of Allexis cauliflora and assess their biological activities. Previous studies have demonstrated antimicrobial properties of this plant. These properties have been mainly attributed to phenolic compounds. However, other plant defense molecules, such as antimicrobial peptides, may be present. In this work, we studied peptide extracts from the leaves of Allexis cauliflora. This plant has also been examined to determine its antimicrobial activities and its minimum inhibitory concentrations against bacteria pathogenic to humans and fungi using the agar well diffusion method. The corresponding concentration value antilog was taken as the minimum inhibitory concentration value. All values of test results were expressed as triple means, mean ± standard deviation. The extract from the leaves of this plant has shown antimicrobial activity against the bacterial and fungal pathogens studied. The phytochemical screening carried out showed the presence of flavonoids and triterpenes. Based on the results obtained, the medicinal value of this plant could be attributed to the presence of secondary metabolites in traditional medicinal plants.

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Section: Antimicrobial Activitymentioning

confidence: 99%

“…A Kirby-Bauer [24] technique was used to screen the antimicrobial activity for the methanolic leaves of Allexis cauliflora.…”

Section: Test Organismsmentioning

confidence: 99%

Qualitative Analysis of Peptides and Biological Activities of Allexis cauliflora (Violaceae) Leaves

Ditchou

Soh

Tchouboun

et al. 2020

JNPR

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“…The Kirby-Bauer method for studying antibiotic resistance is a very easy and reliable test, with few errors in determining resistance, including NFGNB and carbapenems [21,22]. Moreover, the test is run in a solid medium, as occurred with our MIC-dilution method used in disinfectants and antiseptics, permitting a better correlation.…”

Section: Multivariate Analysismentioning

confidence: 99%

Is There A Correlation Between Antibiotic Resistance and Decreased Susceptibility to Biocides in Different Genus of Bacterial Genera?

Herruzo¹,

Herruzo²,

MJ³

2017

JOURNAL OF ANTIBIOTICS RESEARCH

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Journal of Antibiotics ResearchAs WHO warned in 2014 [1], antimicrobial resistance is a global problem. It increases the death risk for patients infected with resistant strains, who consume more healthcare resources than patients infected with sensitive bacteria. For this reason, we explored the possible correlation between antibiotic resistance and decreased susceptibility to biocides, in order to find the best biocide in this environment of increased antibiotic resistance. Objective: Our aim was to assess a possible association between antibiotic resistance and decreased susceptibility to antiseptics or disinfectants, because published papers are contradictory. We used a great number of microorganisms from different bacterial genera and some biocides in order to conclude if there was an association between antibiotic resistances and decreased disinfectant (or antiseptic) effects. Introduction Is AbstractWullt [2] and Russell [3] explain that when we talk of resistance to disinfectants, we are really talking about MIC (minimum inhibitory concentration), since resistance is actually a lack of susceptibility to a given concentration of the compound in 24 hours, whereas the quantities of disinfectant used to carry out a disinfection are large and the times short. Therefore, when we use the word "resistance" we are attempting to express a greater tolerance or a decrease in the susceptibility of a microorganism to the concentration of a disinfectant to which it is normally susceptible [3].Keywords: Antibiotic-resistance; Bacteriostatic-effect; Correlation; ICU-bacteria Material and Methods:Serial dilutions were performed with six disinfectants and two antiseptics to study the MIC-dilution (maximal dilution of disinfectant or antiseptic that inhibits each microorganism) and that dilution was contrasted with the antibiograms of each of the 159 bacteria recently isolated from ICU patients. These antibiograms were determinated by the Kirby-Bauer method, noting only whether they were susceptible or resistant. After, univariate and multivariate analysis were run on the data. Results:With the antibiograms and MIC-dilutions for every bacteria and biocide, the bivariate analysis found that only 4.7% of the antibiotic-biocide pairs showed a significant correlation (p < 0.05%) between antibiotic resistance and decreased susceptibility to the disinfectant or antiseptic, 8.3% showed a significant inverse correlation, and the rest no correlation. Multivariate analysis of the above variables (controlling the effects of the type of microorganism and disinfectant or antiseptic used), showed that overall fit of the equation was very poor, since R 2 = 0.032. Conclusion:In a large sample of different bacteria genera there was no significant correlation between antibiotic resistance and decreased susceptibility to disinfectants and antiseptics, except in 4.7% of the antibiotic-biocide pairs.

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“…The Kirby-Bauer disk diffusion (KBDD) method is more economical and the standardized one used for testing susceptibility to most of the antibiotics. However, only a few studies have provided data on its reliability for testing carbapenems against ABC isolates (1,3).…”

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confidence: 99%

“…According to EUCAST breakpoints (Table 1), no discrepancy was found between the KBDD method and the Etest for all the three carbapenems, though minor error rates could not be calculated for doripenem, since intermediate breakpoints are not defined. Joseph et al (3) showed that the KBDD method can be reliably used for routine testing of meropenem in ABC isolates.…”

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confidence: 99%

Reliability of Kirby-Bauer Disk Diffusion Method for Detecting Carbapenem Resistance in Acinetobacter baumannii-calcoaceticus Complex Isolates

Gautam

Singhal

Arora

et al. 2013

Antimicrob Agents Chemother

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We recently read an article on carbapenem susceptibility testing errors in studies that used three automated systems, disk diffusion (DD), Etest (ET), and broth microdilution (BMD), to investigate Acinetobacter baumannii-calcoaceticus complex (ABC) isolates (1). The authors concluded from the study that the manual methods are more accurate than automated methods. The error rates for testing susceptibility to imipenem and meropenem by DD and the Etest were found to be within an acceptable range compared with MIC detected by BMD. Carbapenems are widely used against multidrug-resistant (MDR) ABC isolates (2). However, the pathogen is associated with increasing carbapenem resistance, which limits therapeutic options and challenges effective hospital infection control. Thus, as antimicrobial resistance increases, accurate susceptibility testing to guide therapeutic options is essential. Agar microdilution and broth microdilution for detecting carbapenem resistance are the recommended methods, but they are impractical to implement as routine tests in many clinical laboratories with a high isolate load that cannot afford automated susceptibility testing or the Etest. The Kirby-Bauer disk diffusion (KBDD) method is more economical and the standardized one used for testing susceptibility to most of the antibiotics. However, only a few studies have provided data on its reliability for testing carbapenems against ABC isolates (1, 3).In the present study, the KBDD method using discs (10 g each) of imipenem (BD), meropenem (Hi-Media), and doripenem (BD) was compared with the bioMérieux Etest (0.002 to 32 g/ml) on 124 nonrepeat MDR ABC strains isolated from blood, respiratory, and pus specimens. The isolates were identified using standard bacteriological procedures (4), and interpretation of susceptibility testing was done according to CLSI guidelines (5). Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 were used as the quality control strains. Taking the Etest as the reference, discordance between it and the KBDD method was categorized as a very major error (reported susceptible when resistant), a major error (reported resistant when susceptible), or a minor error (reported intermediate when resistant or susceptible or vice versa). By the use of CLSI and FDA breakpoints for imipenem and meropenem, no discrepancy was found between the KBDD method and the Etest in the case of imipenem. However, in the case of meropenem, nine strains that were resistant by the KBDD method came out to be intermediately sensitive by the Etest. Thus, the KBDD method showed a minor error rate of 7.3% and no very major or major error for meropenem was recorded, which is acceptable. CLSI does not provide breakpoints for doripenem, but when the same breakpoints used for imipenem and meropenem were applied to doripenem, a major error of 2.5% and a minor error of 21.8% were observed, proving the lower accuracy rate of the KBDD method. According to EUCAST breakpoints (Table 1), no discrepancy was found between the KBDD method and the Et...

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Reliability of Kirby-Bauer disk diffusion method for detecting meropenem resistance among non-fermenting gram-negative bacilli

Abstract: The KBDD method can be reliably used for routine testing of meropenem resistance in A. baumannii, A. lwoffii and P. aeruginosa. However, further studies are needed before employing this technique for detecting meropenem resistance in Pseudomonas spp.

Cited by 22 publications

References 0 publications

Qualitative Analysis of Peptides and Biological Activities of Allexis cauliflora (Violaceae) Leaves

Qualitative Analysis of Peptides and Biological Activities of Allexis cauliflora (Violaceae) Leaves

Is There A Correlation Between Antibiotic Resistance and Decreased Susceptibility to Biocides in Different Genus of Bacterial Genera?

Reliability of Kirby-Bauer Disk Diffusion Method for Detecting Carbapenem Resistance in Acinetobacter baumannii-calcoaceticus Complex Isolates

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