The purpose of this study was both to develop a novel test to measure run, shuttle run and directional change agility, and soccer shots on goal with decision making and to compare it with other agility tests. Multiple comparisons and assessments were conducted, including test-retest, Illinois, Zig-Zag, 30 m, Bosco, T-drill agility, and Wingate peak power tests. A total of 113 Turkish amateur and professional soccer players and tertiary-level students participated in the study. Test-retest and inter-tester reliability testing measures were conducted with athletes. The correlation coefficient of the new test was .88, with no significant difference (p> 0.01> 0.01) between the test results obtained in the first and second test sessions. The results of an analysis of variance revealed a significant (p < 0.01) difference between the T-drill agility and power test results for soccer players. The new agility and skill test is an acceptable and reliable test when considering test-retest reliability and inter-rater reliability. The findings in this study suggest that the novel soccer-specific agility and shooting test can be utilized in the testing and identification of soccer players’ talents.