Background: Acute Appendicitis is inflammation of the appendix, which manifests as pain in the lower right abdomen that can develop into an acute abdomen. This situation can end in the emergency room and require immediate surgical treatment. Ultrasound is the initial choice most often used for the diagnosis of Appendicitis as well as determining whether surgery is needed. Objective: To identify the sensitivity and specificity of ultrasound as an imaging modality for the diagnosis of Acute Appendicitis at PKU Muhammadiyah Gamping Hospital in Yogyakarta. The results of this diagnostic test are essential to be used as an evaluation of the radiology skills, the ultrasound modalities used in health services, as well as being useful for improvement. Method: This research study is a diagnostic test of ultrasound of suspected Acute Appendicitis with a gold standard. The result emerged from the histopathological tissue from the appendectomy. The research subjects consisted of 72 suspected Appendicitis subjected to ultrasound and continued with appendectomy and histopathological in 2014 -2017. The sensitivity and specificity values of the ultrasound were calculated, which were useful for evaluating diagnostic services at PKU Muhammadiyah Gamping Hospital, Yogyakarta. Results: The number of study subjects was 72, with positive ultrasound results of 52 and a negative ultrasound of 20 samples. The 52 respondents with positive ultrasound consisted of 25 adults and 47 children dominated by males. Twenty samples with negative ultrasound results consisted of 9 adults and 11 children dominated by females. The results of sensitivity and specificity values of ultrasound were 82.7%, 33.33%, and 53% of accuracy. The results of this value can be used as an evaluation of ultrasound in cases of Acute Appendicitis in PKU Muhammadiyah Gamping, Hospital, Yogyakarta in Indonesia. Conclusion: Ultrasound has a good sensitivity value for the diagnosis of Acute Appendicitis. However, it has a low specificity value. It shows that the ultrasound can be used as a modality for diagnosing Acute Appendicitis, which has a role in screening the diagnosis. Ultrasound cannot be used as a screening for suspected patients with Acute Appendicitis who are entirely normal or in good condition. It will be the researchers' evaluation of the cause of the low specificity value to improve the diagnosis of suspected Appendicitis.