Reliable and sensitive detection of pancreatic cancer marker by gold nanoflower-based SERS mapping immunoassay

Beyene, Agaje Bedemo; Hwang, Bing‐Joe; Tegegne, Wodaje Addis; Wang, Junsheng; Tsai, Hsieh‐Chih; Su, Wei‐Nien

doi:10.1016/j.microc.2020.105099

Cited by 27 publications

(15 citation statements)

References 33 publications

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“…Then, selectively reducing silver ions adsorbed on the surfaces of nuclei by hydroquinone results in seed-mediated particle growth, as illustrated in Scheme . This is a similar mechanism that involves synthesizing gold nanoflowers by a seed-mediated particle growth approach in the presence of hydroquinone . According to the previously reported study of reduction kinetics, hydroquinone is a weak reducing agent that selectively reduces Au 1+ to Au 0 .…”

Section: Resultsmentioning

confidence: 96%

“…This is a similar mechanism that involves synthesizing gold nanoflowers by a seed-mediated particle growth approach in the presence of hydroquinone. 40 According to the previously reported study of reduction kinetics, 41 hydroquinone is a weak reducing agent that selectively reduces Au 1+ to Au 0 . The reduction takes place spontaneous only on the surface of seeds or nuclei.…”

Section: Preliminary Tests For the Fabrication Of Agmentioning

confidence: 99%

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Cu/Ag Nanoparticle-Based Surface-Enhanced Raman Scattering Substrates for Label-Free Bacterial Detection

Beyene

Tsai

et al. 2022

ACS Appl. Nano Mater.

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Surface-enhanced Raman spectroscopy (SERS) is a promising analytical technique for fast and accurate disease detection due to its attractive features. However, realizing label-free direct detection is still challenging as most probes have extremely low Raman cross sections and little affinity to SERS substrates. Disappointingly, SERS spectra of most bacteria and other biological samples look similar as the differences in their molecular compositions are subtle and not detectable. Hence, the fabrication of highly enhancing plasmonic nanoparticles with excellent uniformity is demanded. Moreover, as SERS substrates are not reusable, cost-effective and simplistic fabrication methods are critical. Here, we report a facile approach to synthesizing Ag nanoparticle array on Cu-foil in less than 3 min, using only Cu-foil, silver nitrate, and hydroquinone. We employed the idea of galvanic replacement in combination with a seedmediated particle-growth approach. The label-free bacterial detection has shown that our Cu/Ag nanoparticle substrate is superior to highly acclaimed Ag nanocubes. Creating strong second and third-generation SERS hot-spots through cooperative interaction of homo-(Ag−Ag) and heterogeneous (Ag−Cu) surfaces contributes mainly to the observed excellent enhancement. Interestingly, direct liquid bacteria sample analysis showed a 6-fold higher detection sensitivity than completely dried samples. We believe that our approach will offer remarkable advantages and change how SERS substrates are prepared and conducted in SERS-based bacterial detection.

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Section: Resultsmentioning

confidence: 96%

Section: Preliminary Tests For the Fabrication Of Agmentioning

confidence: 99%

Cu/Ag Nanoparticle-Based Surface-Enhanced Raman Scattering Substrates for Label-Free Bacterial Detection

Beyene

Tsai

et al. 2022

ACS Appl. Nano Mater.

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“…[78] In recent decades, extensive "sandwich"-based SERS platforms based on antigen-antibody reaction have been constructed for disease-associated proteins detection. [6,[79][80][81] For example, Fu et al proposed a sandwich SERS sensing strategy for the diagnosis of acute myocardial infarction (AMI) by detecting the relevant biomarker cardiac troponin I (cTnI). As The construction and analysis of the recyclable Zn-MOFs-based SERS biosensor for ALP activity.…”

Section: Proteinmentioning

confidence: 99%

Recent Research Progress of Surface‐Enhanced Raman Scattering Dominated Analysis Strategies in Early Diagnosis of Diseases

Chen

Zheng

Tang

et al. 2023

Chemistry An Asian Journal

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Timely and powerful diagnostic means can achieve better therapeutic effects, reduce disease torment, and improve survival rate. As a powerful non‐invasive spectroscopy technology, surface‐enhanced Raman scattering (SERS) have testified to be a great potential candidate for extensive early clinical disease diagnosis. In recent years, the introduction of SERS label, combined with other analysis modes or artificial intelligence, and the emergence of miniaturized devices have made SERS technology more advantageous in early diagnosis of diseases. This review focuses on the research progress of SERS dominated analytical strategies in the field of early disease diagnosis in the past five years. The main content includes the application of label‐free SERS detection; the construction of label SERS methodologies for various disease markers; SERS dominated multimode early disease diagnosis strategies; integration of SERS and artificial intelligence; portable Raman equipment and SERS imaging; and opportunities and trends for SERS diagnostic technology in the future.

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“…According to Tegegne et al, the SERS signal of 1 μM of R6G molecule measured on AgNCs@ polymethylhydrosiloxane (PMHS) modified filter paper declined by 78% after 90 days of exposure to air. The same substrate after the modification with SiO 2 showed improved stability since the SERS signal after 3 months on airdropped by 26% only [ 193 ]. Similar findings were obtained by graphene-coated Ag nanostructures on the surface of the laser-textured Si, after 50 days of exposure of the substrate and R6G analyte to the ambient air, the SERS signal decreased by about 23% [ 128 ].…”

Section: Siliconmentioning

confidence: 99%

Trends in Application of SERS Substrates beyond Ag and Au, and Their Role in Bioanalysis

et al. 2022

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This article compares the applications of traditional gold and silver-based SERS substrates and less conventional (Pd/Pt, Cu, Al, Si-based) SERS substrates, focusing on sensing, biosensing, and clinical analysis. In recent decades plethora of new biosensing and clinical SERS applications have fueled the search for more cost-effective, scalable, and stable substrates since traditional gold and silver-based substrates are quite expensive, prone to corrosion, contamination and non-specific binding, particularly by S-containing compounds. Following that, we briefly described our experimental experience with Si and Al-based SERS substrates and systematically analyzed the literature on SERS on substrate materials such as Pd/Pt, Cu, Al, and Si. We tabulated and discussed figures of merit such as enhancement factor (EF) and limit of detection (LOD) from analytical applications of these substrates. The results of the comparison showed that Pd/Pt substrates are not practical due to their high cost; Cu-based substrates are less stable and produce lower signal enhancement. Si and Al-based substrates showed promising results, particularly in combination with gold and silver nanostructures since they could produce comparable EFs and LODs as conventional substrates. In addition, their stability and relatively low cost make them viable alternatives for gold and silver-based substrates. Finally, this review highlighted and compared the clinical performance of non-traditional SERS substrates and traditional gold and silver SERS substrates. We discovered that if we take the average sensitivity, specificity, and accuracy of clinical SERS assays reported in the literature, those parameters, particularly accuracy (93–94%), are similar for SERS bioassays on AgNP@Al, Si-based, Au-based, and Ag-based substrates. We hope that this review will encourage research into SERS biosensing on aluminum, silicon, and some other substrates. These Al and Si based substrates may respond efficiently to the major challenges to the SERS practical application. For instance, they may be not only less expensive, e.g., Al foil, but also in some cases more selective and sometimes more reproducible, when compared to gold-only or silver-only based SERS substrates. Overall, it may result in a greater diversity of applicable SERS substrates, allowing for better optimization and selection of the SERS substrate for a specific sensing/biosensing or clinical application.

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Reliable and sensitive detection of pancreatic cancer marker by gold nanoflower-based SERS mapping immunoassay

Cited by 27 publications

References 33 publications

Cu/Ag Nanoparticle-Based Surface-Enhanced Raman Scattering Substrates for Label-Free Bacterial Detection

Cu/Ag Nanoparticle-Based Surface-Enhanced Raman Scattering Substrates for Label-Free Bacterial Detection

Recent Research Progress of Surface‐Enhanced Raman Scattering Dominated Analysis Strategies in Early Diagnosis of Diseases

Trends in Application of SERS Substrates beyond Ag and Au, and Their Role in Bioanalysis

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