2015
DOI: 10.1016/j.molbiopara.2015.08.001
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Reliable reference gene selection for quantitative real time PCR in Haemonchus contortus

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Cited by 17 publications
(15 citation statements)
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“…All samples were measured in duplicates with less than 0.5 Cq difference. Relative expression was calculated using ΔΔCt method ( Livak and Schmittgen, 2001 ), using Hc-ama and Hc-gpd as reference genes as recommended by Lecová et al ( Lecova et al, 2015 ).…”
Section: Methodsmentioning
confidence: 99%
“…All samples were measured in duplicates with less than 0.5 Cq difference. Relative expression was calculated using ΔΔCt method ( Livak and Schmittgen, 2001 ), using Hc-ama and Hc-gpd as reference genes as recommended by Lecová et al ( Lecova et al, 2015 ).…”
Section: Methodsmentioning
confidence: 99%
“…qRT-PCR was carried out following the Brilliant III Ultra Fast SYBR QPCR Master Mix protocol (Agilent Technologies, UK, 600882) and results analysed using MxPro qPCR Software, Version 4.10. Gene expression was normalised to ama (HCOI01464300) and gpd (HCOI01760600) ( Lecova et al, 2015 ). Primer sequences are listed in Supplementary Table S2 .…”
Section: Methodsmentioning
confidence: 99%
“…Reference genes superoxide dismutase ( sod ), fatty acid retinol binding protein ( far ) and glyceraldehyde-3P-dehydrogenase ( gpd ) were adopted from Lecova et al (2015) who had shown stable expression of these transcripts in adult H. contortus following varying treatments with albendazole and ivermectin. Primer sequences of both reference and candidate CYP genes can be found in Table S1 along with PCR efficiencies for each primer set.…”
Section: Methodsmentioning
confidence: 99%