2021
DOI: 10.1371/journal.ppat.1009224
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Reliable, scalable functional genetics in bloodstream-form Trypanosoma congolense in vitro and in vivo

Abstract: Animal African trypanosomiasis (AAT) is a severe, wasting disease of domestic livestock and diverse wildlife species. The disease in cattle kills millions of animals each year and inflicts a major economic cost on agriculture in sub-Saharan Africa. Cattle AAT is caused predominantly by the protozoan parasites Trypanosoma congolense and T. vivax, but laboratory research on the pathogenic stages of these organisms is severely inhibited by difficulties in making even minor genetic modifications. As a result, many… Show more

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Cited by 20 publications
(32 citation statements)
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“…Whilst both 2-deoxy-D-glucose and GlcNAc have a detrimental impact on T. congolense viability, knock-down of the glucose transporter array did not affect growth, even though glucose uptake appeared to be reduced by 37% after 72 h of RNAi induction. Consistent with other studies RNAi penetrance does not appear as efficient as in T. brucei [107] and techniques such as CRISPR/Cas9 and conditional knock-out would greatly enhance our capabilities to study this parasite. Nevertheless, these experiments highlight a crucial difference between BSF T. congolense and T. brucei in a pathway that has become a metabolic paradigm in the latter species.…”
Section: Plos Pathogenssupporting
confidence: 85%
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“…Whilst both 2-deoxy-D-glucose and GlcNAc have a detrimental impact on T. congolense viability, knock-down of the glucose transporter array did not affect growth, even though glucose uptake appeared to be reduced by 37% after 72 h of RNAi induction. Consistent with other studies RNAi penetrance does not appear as efficient as in T. brucei [107] and techniques such as CRISPR/Cas9 and conditional knock-out would greatly enhance our capabilities to study this parasite. Nevertheless, these experiments highlight a crucial difference between BSF T. congolense and T. brucei in a pathway that has become a metabolic paradigm in the latter species.…”
Section: Plos Pathogenssupporting
confidence: 85%
“…Consistent with other studies RNAi penetrance does not appear as efficient as in T . brucei [ 107 ] and techniques such as CRISPR/Cas9 and conditional knock-out would greatly enhance our capabilities to study this parasite. Nevertheless, these experiments highlight a crucial difference between BSF T .…”
Section: Discussionmentioning
confidence: 99%
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