Remarkable geographical variations between India and Europe in carriage of the staphylococcal surface protein-encoding sasX/sesI and in the population structure of methicillin-resistant Staphylococcus aureus belonging to clonal complex 8

Backer, Sarah De; Xavier, Basil Britto; Vanjari, Lavanya; Coppens, Jasmine; Lammens, Christine; Lakshmi, V; Carević, Biljana; Hryniewicz, Waleria; Kumar‐Singh, Samir; Lee, A; Harbarth, Stéphan Juergen; Schrenzel, Jacques; Tacconelli, E.; Goossens, Herman; Malhotra-Kumar, Surbhi

doi:10.1016/j.cmi.2018.07.024

Cited by 19 publications

(13 citation statements)

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“…MRSA clonal complex 239 (CC239) comprises the sequence type 239 (ST239) clone, which is widespread globally ( Oliveira et al, 1998 ; Ko et al, 2005 ; Harris et al, 2010 ; Li et al, 2011 ). Several ST239 variants have been previously described, including the Brazilian, Hungarian, Portuguese, Viennese, Indian, Asian, and Eurasian clades ( Harris et al, 2010 ; Wang et al, 2014 ; Monecke et al, 2018 ; De Backer et al, 2019 ). ST239 is endemic in Southeast Asia where it causes more than 70% of hospital-acquired MRSA infections ( Ko et al, 2005 ).…”

Section: Introductionmentioning

confidence: 99%

“…ST239 is endemic in Southeast Asia where it causes more than 70% of hospital-acquired MRSA infections ( Ko et al, 2005 ). Asian ST239 clade isolates are highly virulent and harbour different virulence factors, such as the sasX gene present on a φSPβ-like prophage and encoding a virulence factor involved in colonization and immune evasion of the bacterium ( Wang et al, 2014 ; Nakaminami et al, 2017 ; De Backer et al, 2019 ), which might have facilitated its pandemic spread across Asia.…”

Section: Introductionmentioning

confidence: 99%

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Genomic analysis of methicillin-resistant Staphylococcus aureus clonal complex 239 isolated from Danish patients with and without an international travel history

et al. 2022

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IntroductionInternational travel has been a major determinant for the introduction of pathogens such as methicillin-resistant Staphylococcus aureus (MRSA) into naïve geographic areas. MRSA clonal complex 239 (CC239) is a highly virulent clone that is predominant in Asia. The objective of this study was to determine the geographic origin of MRSA CC239 isolates recovered from Danish cases with or without a history of international travel during 2004–2016.Materials and methodsHuman MRSA isolates with spa types t030 and t037 (n = 60) were obtained from the National Reference Laboratory for Antimicrobial Resistance. For each case, the following data were collected from notification forms: sex, age, isolation year, specimen source (screening swab or clinical sample), infection type, and international travel history. All isolates were whole-genome sequenced, and a comparative genome and phylogenetic analysis was performed.ResultsThe majority of isolates originated from skin and soft tissue (SST) infections and screening swabs. In 31 out of 60 cases reported international travel to different parts of the world. Fifty-four isolates belonged to CC239, including sequence type 239 (ST239) (n = 43), ST241 (n = 5), ST4377 (n = 2), ST4378 (n = 1), ST1465 (n = 1), ST343 (n = 1), and ST592 (n = 1). The majority of the CC239 MRSA isolates (40/54) belonged to well-known geographic clades, including the Asian (n = 12), Serbian (n = 11), South American (n = 2), and Turkish (n = 15). Most MRSA ST239 isolates belonging to the highly virulent Asian clade carried sasX and were recovered from individuals who had travelled to Asia, Africa and the Middle East.ConclusionOur data reveal multiple introductions of MRSA CC239 into Denmark through international travel, which highlights the importance of continued genomic surveillance of MRSA in persons returning from international travel to areas where MRSA is endemic.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genomic analysis of methicillin-resistant Staphylococcus aureus clonal complex 239 isolated from Danish patients with and without an international travel history

et al. 2022

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“…The information on MRSA from Serbia is scarce, mainly reported on the presence and characterization of MRSA isolates from humans and food animals [12,13,14,15,16,17,18], but to date, no study on MRSA presence in companion animals has been published.…”

Section: Introductionmentioning

confidence: 99%

Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia

et al. 2019

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The aim of this study was to characterize a collection of methicillin-resistant Staphylococcus aureus (MRSA) isolates of human and animal origin from Serbia. In total, 36 MRSA isolates—30 obtained from humans and six from companion animals—were investigated by PCR for the presence of antibiotic and biocide resistance determinants and virulence genes (PVL—Panton–Valentine leukocidin, ETs—exfoliative toxins, TSST—toxic shock syndrome toxin, SEs—staphylococcal enterotoxins, and MSCRAMMs—microbial surface components recognizing adhesive matrix molecules and biofilm). Isolates were analyzed by staphylococcal cassette chromosome mec (SCCmec), spa, and dru typing, as well as by multiple locus variable number of tandem repeat analyses (MLVA), multilocus sequence typing (MLST), and subsequently, eBURST. The majority of human MRSA isolates were resistant to gentamicin, erythromycin, clindamycin, and ciprofloxacin. Different antibiotic resistance genes were detected: aac-aphD, ant(6′)-Ia, erm(A), erm(B), erm(C), tet(K), tet(M), fexA, and catpC221. All isolates were susceptible to teicoplanin and linezolid. SCCmec type III was prevalent in human isolates, while SCCmec elements in animals were mostly nontypeable. t037 was the predominant spa type in human and t242 in animal MRSA isolates. The prevalent dru type was dt11c in human and dt10a in animal MRSA isolates. MRSA isolates exhibited 27 different MLVA types. ST239 was predominant in human, while ST5 was prevalent in canine MRSA isolates. PVL was found in two, while tsst-1 was detected in three human isolates. Human-associated clones belonging to ST5, ST45, and ST239 MRSA clones were discovered in companion animals, which suggests anthropozoonotic transmission.

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“…The antibiogram assay was carried out not only in the view of identifying MRSA or ORSA but also to sort out S. aureus causing food poison from stool sample harbouring diverse range of other microbial community and the phage which is specific for Staphylococcus aureus not limiting to MRSA, MDR or ORSA as the genetic adaptability of this bacterium is remarkable and is subjected to a wide change 15 . The anti-susceptibility results emphasize the need for general isolation of phage to specific phage identification.…”

Section: Fig 4: Purification and Concentration Of Staphylococcus Aureumentioning

confidence: 99%

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IJPSR

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Staphylococcus aureus, the facultative anaerobic and opportunistic Bacterium has been characterized as the pathogen with diversified molecular adaptability leading to the emergence of MDR. Advanced use of alternate antibiotics is increasing the scenario of the emergence of Methicillin-Resistant Staphylococcus aureus (MRSA) to Oxacillin Resistant Staphylococcus aureus (ORSA) after that generating associated health infections. Even though Staphylococcus aureus is being considered as one of the important pathogens for its combination of virulence including toxin-mediated multidrug resistance and invasiveness, staphylococcus food poisoning is of high on interest as this infection rate is increasing day by day. Bacteriophage therapy has been of interest due to the absence of secondary resistance and targeted attack and also the lack of recolonization of the pathogen. The present study aims to isolate and purify Staphylococcus aureus-specific bacteriophages from sewage samples and thereby expunging food poison, causing Staphylococcus aureus. On this aspect Staphylococcus aureus causing food poison were isolated from a stool sample of patients at hospitals, and the specific Bacteriophage was isolated from the sewage in Tirunelveli District, India. Enrichment technique improved the stability of the phages. Spot and plaque assay experiments were done to examine the specificity of the isolated phage. Techniques employed result in providing purified specific bacteriophage for Staphylococcus aureus.

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Remarkable geographical variations between India and Europe in carriage of the staphylococcal surface protein-encoding sasX/sesI and in the population structure of methicillin-resistant Staphylococcus aureus belonging to clonal complex 8

Cited by 19 publications

References 31 publications

Genomic analysis of methicillin-resistant Staphylococcus aureus clonal complex 239 isolated from Danish patients with and without an international travel history

Genomic analysis of methicillin-resistant Staphylococcus aureus clonal complex 239 isolated from Danish patients with and without an international travel history

Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia

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