Remarks in Successful Cellular Investigations for Fighting Breast Cancer Using Novel Synthetic Compounds

Shirazi, Farshad H.; Zarghi, Afshin; Kobarfard, Farzad; Zendehdel, Rezvan; Nakhjavani, Maryam; Arfaiee, Sara; Zebardast, Tannaz; Mohebi, Shohreh; Anjidani, Nassim; Ashtarinezhad, Azadeh; Shoeibi, Shahram

doi:10.5772/23005

Cited by 11 publications

(11 citation statements)

References 41 publications

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“…Cell proliferation assay was first performed on MCF7 and MDA-MB-231 cells, confirming the higher proliferation of the latter, in agreement with the literature (Figure 2a) (Gest et al, 2013;H. et al, 2011;Islam & Resat, 2017).…”

Section: Small Extracellular Vesicles Derived From Mda-mb-231 Promote...supporting

confidence: 89%

“…To test such assumption, we examined the effects of small EVs derived from the TNBC MDA-MB-231 breast cancer cells on the stiffness, cytoskeleton organization, nuclear/cellular morphology, and Yap activity of the Luminal A MCF7 breast cancer cells. The two cell lines have been chosen according to their different well-defined metastatic potential: MDA-MB-231 cells are characterized (Islam & Resat, 2017), whereas MCF7 cells are tumorigenic but have low/absent metastatic potential (Gest et al, 2013;H. et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

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Triple negative breast cancer-derived small extracellular vesicles as modulator of biomechanics in target cells

Senigagliesi

Samperi

Cefarin

et al. 2022

Nanomedicine: Nanotechnology, Biology and Medicine

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Section: Small Extracellular Vesicles Derived From Mda-mb-231 Promote...supporting

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Triple negative breast cancer-derived small extracellular vesicles as modulator of biomechanics in target cells

Senigagliesi

Samperi

Cefarin

et al. 2022

Nanomedicine: Nanotechnology, Biology and Medicine

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“…To test such assumption, we examined the effects of small EVs derived from the TNBC MDA-MB-231 breast cancer cells on the stiffness, cytoskeleton organization, nuclear/cellular morphology, and Yap activity of the Luminal A MCF7 breast cancer cells. The two cell lines have been chosen according to their different well-defined metastatic potential: MDA-MB-231 cells are characterized by high proliferation, motility, and metastatic rate (Islam & Resat, 2017), whereas MCF7 cells are tumorigenic but have low/absent metastatic potential (Gest et al, 2013;H. et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

Triple Negative Breast Cancer-derived Small Extracellular Vesicles as Modulator of Biomechanics in target cells

Senigagliesi

Samperi

Cefarin

et al. 2022

Preprint

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Extracellular vesicle (EV) mediated communication has recently been proposed as one of the pivotal routes in the development of cancer metastasis. EVs are nano-sized vesicles swapped between cells, carrying a biologically active content that can promote tumor-induced immune suppression, metastasis and angiogenesis. Thus, EVs constitute a potential target in cancer therapy. However, their role in triggering the premetastatic niche and in tumor spreading is still unclear. Here, we focused on the EV ability to modulate the biomechanical properties of target cells, known to play a crucial role in metastatic spreading. To this purpose, we isolated and thoroughly characterized triple-negative breast cancer (TNBC)-derived small EVs. We then evaluated variations in the mechanical properties (cell stiffness, cytoskeleton/nuclear/morphology and Yap activity rearrangements) of non-metastatic breast cancer MCF7 cells upon EV treatment. Our results suggest that TNBC-derived small EVs are able to directly modify MCF7 cells by inducing a decrease in cell stiffness, rearrangements in cytoskeleton, focal adhesions and nuclear/cellular morphology, and an increase in Yap downstream gene expression. Testing the biomechanical response of cells after EV addition might represent a new functional assay in metastatic cancer framework that can be exploited for future application both in diagnosis and in therapy.

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“…A large majority of published in vitro studies focused on breast cancer and/or estrogen receptor biology have used the MCF-7 human breast cancer cell line. MCF-7 is indeed a commonly used breast cancer cell line, proved to be a suitable in vitro model for breast cancer investigations, including those regarding putative anticancer drugs [80]. Since MCF-7 is a poorly-aggressive and non-invasive cell line, normally being considered to have low metastatic potential [81], it results a good cell system to assay chemopreventive potential of chemicals.…”

Section: Discussionmentioning

confidence: 99%

Pro-Apoptotic Effect of Grape Seed Extract on MCF-7 Involves Transient Increase of Gap Junction Intercellular Communication and Cx43 Up-Regulation: A Mechanism of Chemoprevention

Leone

Longo

Gerardi

et al. 2019

IJMS

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Growing evidence suggests dietary antioxidants reduce the risk of several cancers. Grape seeds extracts (GSE) are a rich source of polyphenols known to have antioxidant, chemopreventive and anticancer properties. Herein, we investigated the in vitro effects and putative action mechanisms of a grape seed extract (GSE) on human breast cancer cells (MCF-7). The effects of GSE were evaluated on cell proliferation, apoptosis and gap-junction-mediated cell-cell communications (GJIC), as basal mechanism involved in the promotion stage of carcinogenesis. GSE (0.05–100 μg/mL) caused a significant dose- and time-dependent inhibition of MCF-7 viability and induced apoptotic cell death, as detected by Annexin-V/Propidium Iodide. Concurrently, GSE induced transient but significant enhancement of GJIC in non-communicating MCF-7 cells, as demonstrated by the scrape-loading/dye-transfer (SL/DT) assay and an early and dose-dependent re-localization of the connexin-43 (Cx43) proteins on plasma membranes, as assayed by immunocytochemistry. Finally, real-time-PCR has evidenced a significant increase in cx43 mRNA expression. The results support the hypothesis that the proliferation inhibition and pro-apoptotic effect of GSE against this breast cancer cell model are mediated by the GJIC improvement via re-localization of Cx43 proteins and up-regulation of cx43 gene, and provide further insight into the action mechanisms underlying the health-promoting action of dietary components.

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Remarks in Successful Cellular Investigations for Fighting Breast Cancer Using Novel Synthetic Compounds

Cited by 11 publications

References 41 publications

Triple negative breast cancer-derived small extracellular vesicles as modulator of biomechanics in target cells

Triple negative breast cancer-derived small extracellular vesicles as modulator of biomechanics in target cells

Triple Negative Breast Cancer-derived Small Extracellular Vesicles as Modulator of Biomechanics in target cells

Pro-Apoptotic Effect of Grape Seed Extract on MCF-7 Involves Transient Increase of Gap Junction Intercellular Communication and Cx43 Up-Regulation: A Mechanism of Chemoprevention

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