Remazol Brilliant Blue R decolourisation by the fungus Pleurotus ostreatus and its oxidative enzymatic system

Palmieri, Gianna; Cennamo, G.; Sannia, Giovanni

doi:10.1016/j.enzmictec.2004.03.026

Cited by 235 publications

(134 citation statements)

References 45 publications

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“…Arantes and Milagres [29] shows the presence of laccase activity in culture media containing Azure B during different incubation periods with L. edodes 0.2 U/ mL (15 days), with P. medullapanis 0.2 U/mL (30 days) and with T. versicolor 0.4 U/mL (17days). Laccase activity has also been reported to be responsible for decolorization of Orange G by I. rasinasum and P. calyptratus [32]. However, in our study, enzymatic activities (Lac and MnP) during the incubation period (15 days), suggest that these enzymes were heavily involved in the decolorization of the two dyes.…”

Section: Decolorization and Enzyme Activities On Liquid Mediacontrasting

confidence: 53%

Biodegradation of Reactive Blue 4 and Orange G by Pycnoporus sanguineus Strain Isolated in Gabon

Attéké

Mounguengui²,

Jean-Bosco

et al. 2013

J Bioremed Biodeg

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The fungus Pycnoporus sanguineus MUCL 51321 white rot isolated in Gabon showed a high ability to decolorize and degrade two synthetic dyes. On solid and liquid media, the fungus had different enzyme activities (laccase and manganese (Mn) peroxidase) in the presence of different substrates. At concentrations of 0.05 g/L (Orange G) and 0.3 g/L (reactive blue 4), the respective rates of decolorization of 81% and 97% were observed after 15 days of incubation in liquid media. In the same time interval, changes on the spectra (UV-vis and FTIR) and chromatograms (HPLC) showed that these decolorizations were due to the degradation of dyes by fungus with the production of new compounds. The study revealed the possibility of the use of this fungal strain in the microbial degradation process of synthetic dyes. Aldrich (Sigma-Aldrich, St. Louis, MO, USA). All reagents used were of the analytical grade. Journal of Bior emediation & Biodegradation Culture ConditionsThe composition of the solid growth medium used was as follows: 0.2% glucose, 0.2% malt extract, 0.01% MgSO 4 , 0.01% MnCl 2 , 0.03% NH 4 NO 3 , 0.026% KH 2 PO 4 , 0.026% Na 2 HPO 4 , 0.01% CaCl 2 .2H 2 O, 0.0001% FeC1 3 .6H 2 O, 0.0001% ZnCl 2 , 1.6% and 1.6% agar in 1 L of distilled water. The pH was adjusted to 5.5 with hydrochloric acid (0. 5 N) before autoclaving (at 121°C for 15 min). The culture medium was transferred into 4 Erlenmeyer flasks (200 mL each) and each reagent (enzymatic revelation) or dye, depending on its final concentration was added. The mixture was shaken, then about 15 mL were distributed in a Petri dish (diameter 8.5 mm); each test was replicated three times. 50 mL of liquid culture media in flasks of 100 mL were constituted in almost the same way as the solid culture media described above, except for the absence of agar. Each test was conducted three times. Decolorization experimentsDyes decolorization on solid medium: Solid culture media contained different concentrations of each dye (0.05 g/L to 1.5 g/L). Various controls were also prepared: some Petri dishes with fungus alone and others with dye alone to assess possible abiotic decolorization. One square portion of agar (1 cm of diameter) containing the fungus was placed at the centre of each Petri dish. The fungal growth diameters and the halos of decolorization were measured every day during one week at constants temperature (30°C) and humidity (75%). After these measures, different ratios and rates (decolorization or inhibition) were determined as described by . The optimum temperature of decolorization was evaluated using different growth temperatures (15, 22, 30, 35, 40 and 45°C). Moreover, the optimal concentrations for decolorization (0.05 g/L for the Orange G and 0.3 g/L for the RB4) were obtained after one week. All tests were conducted three times (averages are presented with the corresponding standard deviations).Dyes decolorization on liquid culture medium: For each Erlenmeyer flask (100 mL) containing liquid culture medium (50 mL) and dye (0.05 g/L Orange G or 0.3...

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Section: Decolorization and Enzyme Activities On Liquid Mediacontrasting

confidence: 53%

Biodegradation of Reactive Blue 4 and Orange G by Pycnoporus sanguineus Strain Isolated in Gabon

Attéké

Mounguengui²,

Jean-Bosco

et al. 2013

J Bioremed Biodeg

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“…The ability of the crude P. ostreatus extract to degrade high concentrations of RBBR with only one addition of H2O2 demonstrates its high biotechnological potential. The ability to decolorize high concentrations of RBBR added during successive cycles has also been observed during the growth of Irpex lacteus and P. ostreatus (14,18).…”

Section: Successive Additions Of Rbbrmentioning

confidence: 87%

Biodegradation of remazol brilliant blue R by ligninolytic enzymatic complex produced by Pleurotus ostreatus

Machado¹,

Matheus²

2006

Braz. J. Microbiol.

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Pleurotus ostreatus ("shimeji") is produced in Brazil on a commercial scale using various lignocellulosic residues. Efforts have been made to reuse the culture residue to obtain products of greater aggregate value such as enzymes or in processes of bioremediation. We evaluated the Remazol brilliant blue R (RBBR) degradation potential of extracts from solid substrate colonized by P. ostreatus and extracts from residue of the "shimeji" mushroom yield. Colonized substrates and residue were provided by Toyobo do Brasil Ltda. Extraction was performed with sodium acetate buffer (50 mM, pH 4.6). RBBR decolorization was monitored at 592 nm and peroxidase and laccase activities were measured by monitoring the oxidation of ABTS. Horseradish peroxidase was used as reference. The time of growth of P. ostreatus influenced RBBR degradation and peroxidase and laccase activities. Concentration of 1 mM H2O2 and pH 4.0 were the best for RBBR decolorization. Complete RBBR decolorization was obtained with the addition of only one aliquot of 50 µL of 1 mM H2O2. The stability of the extracts was higher when they were kept under refrigeration than when stored frozen. The potential application of the ligninolytic complex derived from P. ostreatus and mushroom residue for xenobiotic degradation was demonstrated.

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“…The pH of the liquid medium will change the charge of the enzyme thus influences the adsorption of charge dye groups to it. The absorption of acidic dye was optimum at acidic condition because there was an increased in the protonation of weak base group (biomass) that bound and degraded the anionic group of acidic dye (Palmieri et al 2005). …”

Section: Effect Of Initial Dye Concentration On the Decolorization Ofmentioning

confidence: 99%

Microbial Decolorization of an Azo Dye Reactive Black 5 Using White-Rot Fungus Pleurotus eryngii F032

Hadibarata

Adnan

Yusoff

et al. 2013

Water Air Soil Pollut

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The growth of white-rot fungus Pleurotus eryngii F032 in a suitable medium can degrade an azo dye Reactive Black 5 (RB5), because of its ability to produce ligninolytic enzymes such as lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase that able to degrade and transform the complex structure of the dye into a less toxic compound. The effect of environmental factors such as initial concentration of Reactive Black 5, pH, temperature of growth medium, surfactant (Tween 80), and agitation were also investigated. The productions of ligninolytic enzymes were enhanced by increasing the white-rot fungi growth in optimum conditions. The decolorization of Reactive Black 5 were analyzed by using UV-vis spectrophotometer at the maximum absorbance of 596 nm. The whiterot fungus, P. eryngii F032 culture exhibited 93.56 % decolorization of 10 mg/L RB5 within 72 h of incubation in dark condition with agitation. The optimum pH and temperature for the decolorizing activity was recorded at pH 3 and 40°C, respectively. The addition of surfactant (Tween 80) increased the decolorization to 93.57 % and agitation of growth medium at 120 rpm enhanced the distribution of nutrients to the fungus thus optimized the enzymatic reaction that resulted maximum decolorization of RB5 which was 93.57 %. The molecular docking studies were performed using Chimera visualization software as to analyze the decolorization mechanism of RB5 at molecular level.

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Remazol Brilliant Blue R decolourisation by the fungus Pleurotus ostreatus and its oxidative enzymatic system

Cited by 235 publications

References 45 publications

Biodegradation of Reactive Blue 4 and Orange G by Pycnoporus sanguineus Strain Isolated in Gabon

Biodegradation of Reactive Blue 4 and Orange G by Pycnoporus sanguineus Strain Isolated in Gabon

Biodegradation of remazol brilliant blue R by ligninolytic enzymatic complex produced by Pleurotus ostreatus

Microbial Decolorization of an Azo Dye Reactive Black 5 Using White-Rot Fungus Pleurotus eryngii F032

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